Strain Name:
C57BL/6J-MtgxR8027Btlr/Mmmh
Stock Number:
067466-MU
Citation ID:
RRID:MMRRC_067466-MU
Other Names:
R8027 (G1)
Major Collection:

Strain Information

Wwp2
Name: WW domain containing E3 ubiquitin protein ligase 2
Synonyms: 1300010O06Rik, AIP2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66894
Homologene: 48490
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Grhl2
Name: grainyhead like transcription factor 2
Synonyms: BOM, clft3, grainyheadlike, Tcfcp2l3, 0610015A08Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 252973
HGNC: HGNC:2799
Homologene: 32616
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Prpf40a
Name: pre-mRNA processing factor 40A
Synonyms: FBP11, Fnbp3, 2810012K09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56194
Homologene: 6377
Fndc3a
Name: fibronectin type III domain containing 3A
Synonyms: 1700094E19Rik, F730017H24Rik, Fndc3, D14Ertd453e, sys
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319448
Homologene: 8952
Mark4
Name: MAP/microtubule affinity regulating kinase 4
Synonyms: Markl1, 2410090P21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232944
Homologene: 57146
Bmpr2
Name: bone morphogenetic protein receptor type 2
Synonyms: BMPR-II, BMPRII, BMP-2, 2610024H22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12168
HGNC: HGNC:1078
Homologene: 929
Utp25
Name: UTP25 small subunit processome component
Synonyms: AA408296, Diexf, mDef
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215193
Homologene: 6170
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
C2cd5
Name: C2 calcium-dependent domain containing 5
Synonyms: C030008B15Rik, CDP138, 5730419I09Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74741
Homologene: 8873
Gapdh
Name: glyceraldehyde-3-phosphate dehydrogenase
Synonyms: Gapd, Gapd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14433
HGNC: HGNC:4141
Homologene: 107053
Ptprm
Name: protein tyrosine phosphatase receptor type M
Synonyms: RPTPmu
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19274
VEGA: 17
HGNC: HGNC:9675
Homologene: 37694
Leng8
Name: leukocyte receptor cluster (LRC) member 8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232798
Homologene: 13612
Cog1
Name: component of oligomeric golgi complex 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16834
HGNC: HGNC:6545
Homologene: 8411
Nudt9
Name: nudix hydrolase 9
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 9, 1190002C07Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74167
HGNC: HGNC:8056
Homologene: 11435
Inpp5f
Name: inositol polyphosphate-5-phosphatase F
Synonyms: SAC2, 5830435P03Rik, cI-27
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101490
Homologene: 8962
Slc5a2
Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
Synonyms: Sglt2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246787
Homologene: 2289
Tulp3
Name: TUB like protein 3
Synonyms: 2310022L06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22158
Homologene: 20711
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Astn2
Name: astrotactin 2
Synonyms: Astnl, 1d8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56079
Homologene: 77850
Rimoc1
Name: RAB7A interacting MON1-CCZ1 complex subunit 1
Synonyms: AW549877
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 106064
VEGA: 15
Homologene: 27085
Izumo1
Name: izumo sperm-egg fusion 1
Synonyms: 1700058F15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73456
Homologene: 77701
Abhd18
Name: abhydrolase domain containing 18
Synonyms: 3110057O12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269423
Homologene: 105457
Adam5
Name: a disintegrin and metallopeptidase domain 5
Synonyms: tMDCII
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11499
HGNC: HGNC:212
Homologene: 49138
Slc13a2
Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
Synonyms: mNaDC-1, sodium/dicarboxylate co-transporter, Nadc1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20500
Homologene: 2965
Grik1
Name: glutamate receptor, ionotropic, kainate 1
Synonyms: GluK5, D16Ium24, Glurbeta1, Glur-5, D16Ium24e, A830007B11Rik, Glur5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14805
HGNC: HGNC:4579
Homologene: 68992
Sh3pxd2b
Name: SH3 and PX domains 2B
Synonyms: Fad49, Tks4, G431001E03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268396
Homologene: 27952
Fut1
Name: fucosyltransferase 1
Synonyms: H transferase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14343
HGNC: HGNC:4012
Homologene: 120
Pcdhb9
Name: protocadherin beta 9
Synonyms: Pcdhb4C, PcdhbI
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93880
HGNC: HGNC:8689
Homologene: 87124
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 639774
Homologene: 106613
Timd5
Name: T cell immunoglobulin and mucin domain containing 5
Synonyms: Gm12169
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 210535
Homologene: 130033
Hsp90b1
Name: heat shock protein 90, beta (Grp94), member 1
Synonyms: tumor rejection antigen (gp96) 1, Tra1, 90 kDa, GRP94, Targ2, ERp99, Tra-1, endoplasmin, gp96
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22027
Homologene: 2476
Lztr1
Name: leucine-zipper-like transcriptional regulator, 1
Synonyms: TCFL2, 1200003E21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66863
HGNC: HGNC:6742
Homologene: 4925
Fam107a
Name: family with sequence similarity 107, member A
Synonyms: DRR1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268709
Homologene: 48509
Skint4
Name: selection and upkeep of intraepithelial T cells 4
Synonyms: 9530098N22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320640
Aldh5a1
Name: aldhehyde dehydrogenase family 5, subfamily A1
Synonyms: OTTMUSG00000000613, 6330403E24Rik, D630032B01Rik, SSADH
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 214579
HGNC: HGNC:408
Homologene: 840
Fam161a
Name: family with sequence similarity 161, member A
Synonyms: 4930430E16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 73873
Homologene: 19131
Calcoco2
Name: calcium binding and coiled-coil domain 2
Synonyms: Ndp52l1, 2410154J16Rik, Ndp52
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76815
Ube2e2
Name: ubiquitin-conjugating enzyme E2E 2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218793
Homologene: 77337
Fbxl8
Name: F-box and leucine-rich repeat protein 8
Synonyms: FBL8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50788
Homologene: 9279
Tmem198
Name: transmembrane protein 198
Synonyms: Tmem198-1, Tmem198a, A230078I05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319998
Homologene: 44245
Gm5591
Name: predicted gene 5591
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434171
Homologene: 80005
Gzmk
Name: granzyme K
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14945
HGNC: HGNC:4711
Homologene: 20485
Or55b10
Name: olfactory receptor family 55 subfamily B member 10
Synonyms: GA_x6K02T2PBJ9-5216160-5215210, S50, Olfr545, MOR42-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258837
Homologene: 17410
Psg29
Name: pregnancy-specific beta-1-glycoprotein 29
Synonyms: cea17
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 114872
Homologene: 83380
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 59,867,803 bp
  • A to T, chromosome 1 at 75,480,062 bp
  • A to G, chromosome 1 at 193,118,222 bp
  • A to G, chromosome 2 at 53,191,138 bp
  • A to G, chromosome 2 at 76,896,716 bp
  • C to T, chromosome 3 at 40,933,723 bp
  • T to A, chromosome 3 at 86,417,912 bp
  • A to C, chromosome 4 at 65,540,971 bp
  • T to A, chromosome 4 at 111,928,739 bp
  • G to T, chromosome 4 at 112,157,985 bp
  • T to A, chromosome 5 at 104,064,927 bp
  • C to T, chromosome 6 at 113,546,622 bp
  • A to G, chromosome 6 at 125,162,368 bp
  • T to C, chromosome 6 at 128,334,473 bp
  • A to G, chromosome 6 at 143,078,320 bp
  • CT to C, chromosome 7 at 4,142,856 bp
  • G to A, chromosome 7 at 17,208,640 bp
  • T to C, chromosome 7 at 19,447,239 bp
  • T to C, chromosome 7 at 38,522,298 bp
  • G to A, chromosome 7 at 45,618,865 bp
  • T to C, chromosome 7 at 45,626,254 bp
  • T to A, chromosome 7 at 102,494,422 bp
  • A to G, chromosome 7 at 128,270,546 bp
  • T to C, chromosome 7 at 128,690,673 bp
  • T to C, chromosome 8 at 24,782,558 bp
  • C to T, chromosome 8 at 105,268,126 bp
  • C to A, chromosome 8 at 107,555,477 bp
  • T to A, chromosome 9 at 95,865,756 bp
  • T to C, chromosome 9 at 119,633,790 bp
  • A to G, chromosome 10 at 86,696,730 bp
  • A to T, chromosome 11 at 23,020,125 bp
  • C to T, chromosome 11 at 32,422,210 bp
  • T to C, chromosome 11 at 46,537,917 bp
  • T to A, chromosome 11 at 78,404,756 bp
  • A to T, chromosome 11 at 96,100,415 bp
  • T to C, chromosome 11 at 113,652,389 bp
  • A to T, chromosome 13 at 24,926,110 bp
  • T to A, chromosome 13 at 113,171,900 bp
  • A to T, chromosome 14 at 8,298,813 bp
  • A to C, chromosome 14 at 18,574,317 bp
  • A to G, chromosome 14 at 72,553,543 bp
  • T to C, chromosome 15 at 3,986,212 bp
  • A to G, chromosome 15 at 37,279,483 bp
  • T to C, chromosome 16 at 17,512,112 bp
  • T to G, chromosome 16 at 87,936,005 bp
  • T to C, chromosome 17 at 66,944,205 bp
  • T to C, chromosome 18 at 37,403,016 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8027 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067466-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.