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Strain Name:
C57BL/6J-MtgxR8029Btlr/Mmmh
Stock Number:
067468-MU
Citation ID:
RRID:MMRRC_067468-MU
Other Names:
R8029 (G1)
Major Collection:

Strain Information

Vps41
Name: VPS41 HOPS complex subunit
Synonyms: Vam2, mVam2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218035
VEGA: 13
Homologene: 69165
Acvr1c
Name: activin A receptor, type IC
Synonyms: ALK7, Alk-7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269275
Homologene: 26724
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Get3
Name: guided entry of tail-anchored proteins factor 3, ATPase
Synonyms: ArsA, 1810048H22Rik, Asna1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56495
HGNC: HGNC:752
Homologene: 31513
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 55,696,078 bp
  • G to A, chromosome 1 at 58,343,668 bp
  • T to C, chromosome 1 at 138,078,459 bp
  • A to G, chromosome 1 at 139,471,632 bp
  • A to G, chromosome 1 at 171,386,687 bp
  • A to G, chromosome 1 at 183,089,288 bp
  • A to C, chromosome 1 at 194,958,552 bp
  • A to T, chromosome 2 at 28,613,675 bp
  • A to G, chromosome 2 at 58,296,117 bp
  • A to G, chromosome 2 at 87,810,032 bp
  • A to T, chromosome 2 at 119,528,723 bp
  • T to C, chromosome 2 at 127,741,116 bp
  • T to C, chromosome 2 at 152,920,299 bp
  • C to A, chromosome 2 at 166,575,603 bp
  • C to T, chromosome 3 at 22,200,436 bp
  • C to A, chromosome 3 at 61,003,522 bp
  • T to C, chromosome 3 at 72,921,142 bp
  • T to C, chromosome 3 at 127,729,285 bp
  • C to T, chromosome 3 at 133,082,107 bp
  • T to C, chromosome 3 at 146,240,963 bp
  • T to C, chromosome 4 at 123,444,892 bp
  • C to T, chromosome 5 at 104,050,611 bp
  • T to C, chromosome 5 at 108,669,412 bp
  • T to A, chromosome 5 at 112,961,642 bp
  • G to T, chromosome 5 at 135,064,332 bp
  • A to G, chromosome 6 at 5,057,518 bp
  • T to A, chromosome 6 at 71,912,956 bp
  • A to G, chromosome 6 at 122,081,545 bp
  • C to T, chromosome 6 at 125,144,420 bp
  • A to G, chromosome 7 at 13,036,597 bp
  • G to A, chromosome 7 at 14,121,628 bp
  • C to T, chromosome 7 at 24,756,169 bp
  • T to C, chromosome 7 at 24,919,738 bp
  • T to C, chromosome 7 at 82,270,883 bp
  • T to A, chromosome 7 at 108,713,830 bp
  • C to G, chromosome 7 at 127,786,214 bp
  • A to T, chromosome 8 at 61,877,312 bp
  • T to C, chromosome 8 at 85,019,827 bp
  • A to G, chromosome 8 at 104,834,850 bp
  • C to A, chromosome 8 at 119,547,805 bp
  • T to C, chromosome 9 at 19,035,794 bp
  • A to G, chromosome 9 at 20,314,347 bp
  • A to G, chromosome 9 at 49,470,251 bp
  • T to C, chromosome 9 at 110,867,487 bp
  • A to T, chromosome 10 at 41,927,000 bp
  • A to T, chromosome 11 at 31,101,180 bp
  • T to C, chromosome 11 at 34,822,592 bp
  • C to T, chromosome 11 at 35,821,165 bp
  • G to T, chromosome 11 at 67,211,240 bp
  • T to A, chromosome 11 at 87,731,894 bp
  • G to T, chromosome 11 at 94,409,738 bp
  • G to A, chromosome 11 at 95,474,367 bp
  • A to C, chromosome 12 at 35,119,886 bp
  • T to C, chromosome 12 at 113,329,145 bp
  • C to T, chromosome 13 at 18,823,785 bp
  • A to G, chromosome 13 at 21,445,350 bp
  • T to C, chromosome 13 at 59,742,377 bp
  • T to C, chromosome 13 at 77,029,886 bp
  • T to C, chromosome 14 at 47,416,440 bp
  • T to A, chromosome 14 at 54,639,399 bp
  • T to C, chromosome 14 at 62,353,548 bp
  • A to G, chromosome 15 at 31,496,002 bp
  • T to C, chromosome 15 at 76,305,696 bp
  • A to G, chromosome 16 at 90,779,152 bp
  • T to A, chromosome 17 at 23,477,770 bp
  • A to T, chromosome 17 at 30,135,433 bp
  • G to T, chromosome 17 at 67,817,594 bp
  • T to A, chromosome 18 at 11,054,944 bp
  • T to G, chromosome 18 at 12,914,521 bp
  • C to T, chromosome 18 at 20,032,274 bp
  • A to G, chromosome 18 at 59,179,447 bp
  • A to G, chromosome 18 at 89,090,474 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8029 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067468-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.