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Strain Name:
C57BL/6J-MtgxR8051Btlr/Mmmh
Stock Number:
067488-MU
Citation ID:
RRID:MMRRC_067488-MU
Other Names:
R8051 (G1)
Major Collection:

Strain Information

Tyrp1
Name: tyrosinase-related protein 1
Synonyms: Tyrp, isa, TRP-1, TRP1, Oca3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22178
Homologene: 464
Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: PSGAP-s, PSGAP-m, A930033B01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78514
Homologene: 12695
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Spns1
Name: SPNS lysolipid transporter 1, lysophospholipid
Synonyms: 2210013K02Rik, spinster homolog
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73658
Homologene: 41530
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Tmem121
Name: transmembrane protein 121
Synonyms: Hole, 2410008J05Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 69195
Homologene: 11900
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 51,301,124 bp
  • A to T, chromosome 1 at 130,655,968 bp
  • T to C, chromosome 1 at 193,330,067 bp
  • C to T, chromosome 2 at 30,030,159 bp
  • T to C, chromosome 2 at 90,777,739 bp
  • T to A, chromosome 2 at 103,970,700 bp
  • C to T, chromosome 2 at 125,306,463 bp
  • T to C, chromosome 2 at 129,208,200 bp
  • T to C, chromosome 3 at 53,535,355 bp
  • C to T, chromosome 4 at 46,736,349 bp
  • C to A, chromosome 4 at 80,837,660 bp
  • A to G, chromosome 5 at 28,352,625 bp
  • A to T, chromosome 5 at 73,669,720 bp
  • A to G, chromosome 5 at 81,465,266 bp
  • G to A, chromosome 5 at 113,819,441 bp
  • T to G, chromosome 5 at 147,358,955 bp
  • T to C, chromosome 5 at 147,582,691 bp
  • A to G, chromosome 6 at 41,305,062 bp
  • T to C, chromosome 6 at 67,535,399 bp
  • G to T, chromosome 6 at 113,459,367 bp
  • A to T, chromosome 7 at 44,242,552 bp
  • T to A, chromosome 7 at 79,100,779 bp
  • T to C, chromosome 7 at 126,372,536 bp
  • T to A, chromosome 7 at 141,104,532 bp
  • T to C, chromosome 8 at 22,910,249 bp
  • A to G, chromosome 8 at 77,517,680 bp
  • A to G, chromosome 8 at 84,033,949 bp
  • T to A, chromosome 10 at 79,867,004 bp
  • C to A, chromosome 11 at 70,031,642 bp
  • G to T, chromosome 11 at 74,395,825 bp
  • G to A, chromosome 11 at 78,273,412 bp
  • A to T, chromosome 11 at 99,337,846 bp
  • T to G, chromosome 11 at 103,503,126 bp
  • T to A, chromosome 12 at 111,781,950 bp
  • C to T, chromosome 12 at 113,188,867 bp
  • G to A, chromosome 13 at 21,484,653 bp
  • A to G, chromosome 13 at 30,761,473 bp
  • T to A, chromosome 13 at 49,193,812 bp
  • C to A, chromosome 14 at 50,515,643 bp
  • T to C, chromosome 14 at 52,268,696 bp
  • A to T, chromosome 16 at 17,792,579 bp
  • C to A, chromosome 17 at 25,723,479 bp
  • T to A, chromosome 17 at 34,363,499 bp
  • T to A, chromosome 17 at 37,009,893 bp
  • G to A, chromosome 17 at 37,466,322 bp
  • A to T, chromosome 17 at 40,232,560 bp
  • A to T, chromosome 18 at 19,981,213 bp
  • T to A, chromosome 19 at 5,476,067 bp
  • C to T, chromosome X at 37,901,376 bp
  • A to T, chromosome Y at 2,117,380 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8051 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067488-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.