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Strain Name:
C57BL/6J-MtgxR8076Btlr/Mmmh
Stock Number:
067510-MU
Citation ID:
RRID:MMRRC_067510-MU
Other Names:
R8076 (G1)
Major Collection:

Strain Information

Chrm4
Name: cholinergic receptor, muscarinic 4
Synonyms: Chrm-4, muscarinic acetylcholine receptor 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12672
HGNC: HGNC:1953
Homologene: 20192
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Olig2
Name: oligodendrocyte transcription factor 2
Synonyms: RK17, Olg-2, Bhlhb1, bHLHe19
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50913
HGNC: HGNC:9398
Homologene: 4241
Sort1
Name: sortilin 1
Synonyms: sortilin, neurotensin receptor 3, Ntsr3, Ntr3, 2900053A11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20661
Homologene: 136097
Arid4b
Name: AT-rich interaction domain 4B
Synonyms: RBBP1L1, SAP180, BRCAA1, Rbp1l1, 6720480E17Rik, 6330417L24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94246
Homologene: 12847
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 63,306,101 bp
  • C to T, chromosome 1 at 153,907,122 bp
  • T to A, chromosome 1 at 174,187,231 bp
  • G to A, chromosome 2 at 26,224,885 bp
  • C to A, chromosome 2 at 26,990,612 bp
  • A to T, chromosome 2 at 87,418,545 bp
  • A to G, chromosome 2 at 91,927,859 bp
  • C to A, chromosome 2 at 155,807,561 bp
  • A to G, chromosome 2 at 173,155,278 bp
  • T to A, chromosome 3 at 96,802,346 bp
  • T to C, chromosome 3 at 108,338,867 bp
  • G to T, chromosome 3 at 108,492,082 bp
  • T to C, chromosome 3 at 146,190,414 bp
  • T to C, chromosome 4 at 62,191,316 bp
  • T to A, chromosome 4 at 136,268,129 bp
  • T to C, chromosome 4 at 148,525,803 bp
  • A to G, chromosome 5 at 17,727,364 bp
  • T to C, chromosome 5 at 53,642,137 bp
  • G to A, chromosome 5 at 138,283,142 bp
  • A to G, chromosome 6 at 111,566,039 bp
  • A to G, chromosome 6 at 136,804,126 bp
  • G to A, chromosome 7 at 46,710,607 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • A to G, chromosome 7 at 101,343,969 bp
  • T to C, chromosome 7 at 104,340,830 bp
  • A to T, chromosome 7 at 105,755,921 bp
  • A to G, chromosome 7 at 105,761,982 bp
  • C to T, chromosome 7 at 141,091,872 bp
  • T to A, chromosome 8 at 4,135,195 bp
  • T to A, chromosome 8 at 24,962,922 bp
  • T to A, chromosome 8 at 88,729,718 bp
  • T to C, chromosome 8 at 127,415,596 bp
  • C to T, chromosome 9 at 24,680,692 bp
  • T to C, chromosome 9 at 56,127,780 bp
  • G to A, chromosome 9 at 119,966,326 bp
  • A to C, chromosome 10 at 82,296,686 bp
  • T to A, chromosome 11 at 36,027,221 bp
  • A to T, chromosome 11 at 40,685,265 bp
  • T to C, chromosome 11 at 88,964,970 bp
  • A to G, chromosome 11 at 115,846,929 bp
  • A to G, chromosome 12 at 55,565,911 bp
  • A to G, chromosome 12 at 101,883,482 bp
  • A to G, chromosome 12 at 103,416,218 bp
  • A to G, chromosome 12 at 113,360,538 bp
  • A to T, chromosome 13 at 14,186,950 bp
  • A to G, chromosome 13 at 95,017,330 bp
  • A to T, chromosome 14 at 67,802,977 bp
  • A to G, chromosome 14 at 70,557,941 bp
  • A to T, chromosome 15 at 99,943,470 bp
  • C to A, chromosome 16 at 91,226,411 bp
  • A to G, chromosome 17 at 30,784,153 bp
  • A to G, chromosome 17 at 63,060,365 bp
  • C to T, chromosome 18 at 58,026,424 bp
  • T to A, chromosome 19 at 3,978,859 bp
  • C to T, chromosome 19 at 36,440,083 bp
  • A to G, chromosome 19 at 56,843,835 bp
  • A to T, chromosome 19 at 60,773,925 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8076 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067510-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.