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Strain Name:
C57BL/6J-MtgxR8100Btlr/Mmmh
Stock Number:
067532-MU
Citation ID:
RRID:MMRRC_067532-MU
Other Names:
R8100 (G1)
Major Collection:

Strain Information

Sf1
Name: splicing factor 1
Synonyms: WBP4, MZFM, CW17R, Zfp162
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22668
Homologene: 134065
Chrna6
Name: cholinergic receptor, nicotinic, alpha polypeptide 6
Synonyms: Acra6, alpha6 nAChR
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11440
Homologene: 20888
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Cdk7
Name: cyclin dependent kinase 7
Synonyms: CRK4 PK (CDC2-related-kinase-4 protein kinase), Crk4, Cdkn7
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12572
VEGA: 13
HGNC: HGNC:1778
Homologene: 1363
Zbtb32
Name: zinc finger and BTB domain containing 32
Synonyms: Tzfp, 4930524C15Rik, PLZP, Rog
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 58206
Homologene: 8661
Cdk13
Name: cyclin dependent kinase 13
Synonyms: 2310015O17Rik, Cdc2l5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69562
VEGA: 13
HGNC: HGNC:1733
Homologene: 135707
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 150,102,721 bp
  • T to C, chromosome 1 at 155,953,402 bp
  • A to T, chromosome 1 at 166,401,942 bp
  • A to C, chromosome 1 at 173,595,182 bp
  • G to A, chromosome 2 at 89,835,685 bp
  • C to T, chromosome 2 at 104,256,942 bp
  • T to A, chromosome 2 at 121,360,777 bp
  • TCTCCTC to TCTC, chromosome 2 at 121,439,456 bp
  • C to G, chromosome 2 at 140,275,409 bp
  • T to A, chromosome 2 at 160,698,235 bp
  • A to G, chromosome 2 at 164,237,792 bp
  • A to G, chromosome 2 at 168,231,337 bp
  • G to A, chromosome 3 at 95,881,344 bp
  • A to G, chromosome 4 at 107,383,605 bp
  • A to C, chromosome 4 at 112,626,000 bp
  • T to G, chromosome 5 at 114,111,184 bp
  • A to T, chromosome 6 at 23,838,809 bp
  • C to T, chromosome 6 at 82,730,878 bp
  • G to A, chromosome 7 at 23,808,587 bp
  • T to A, chromosome 7 at 24,261,177 bp
  • CTTG to CTTGTTG, chromosome 7 at 30,591,521 bp
  • T to C, chromosome 7 at 45,833,747 bp
  • T to G, chromosome 7 at 81,024,192 bp
  • T to C, chromosome 7 at 102,107,198 bp
  • C to T, chromosome 7 at 127,393,324 bp
  • A to T, chromosome 8 at 21,326,974 bp
  • C to T, chromosome 8 at 27,413,816 bp
  • T to A, chromosome 8 at 43,138,680 bp
  • A to G, chromosome 8 at 69,140,745 bp
  • A to G, chromosome 8 at 77,604,782 bp
  • T to C, chromosome 9 at 79,774,150 bp
  • C to T, chromosome 9 at 105,878,640 bp
  • A to G, chromosome 9 at 111,392,793 bp
  • G to A, chromosome 9 at 115,167,254 bp
  • C to A, chromosome 10 at 27,041,117 bp
  • T to C, chromosome 10 at 58,490,648 bp
  • C to A, chromosome 11 at 21,999,392 bp
  • A to C, chromosome 11 at 48,849,519 bp
  • G to A, chromosome 11 at 60,517,190 bp
  • T to A, chromosome 12 at 98,682,622 bp
  • A to G, chromosome 12 at 117,966,633 bp
  • C to T, chromosome 13 at 17,803,516 bp
  • T to C, chromosome 13 at 100,706,417 bp
  • G to A, chromosome 14 at 55,772,265 bp
  • A to T, chromosome 14 at 101,900,463 bp
  • CAGCTGGAGGAGC to CAGC, chromosome 15 at 78,436,913 bp
  • T to C, chromosome 15 at 83,016,529 bp
  • T to A, chromosome 15 at 85,285,156 bp
  • T to A, chromosome 16 at 58,717,713 bp
  • A to G, chromosome 16 at 87,436,621 bp
  • A to G, chromosome 17 at 7,771,853 bp
  • C to A, chromosome 17 at 70,846,549 bp
  • A to G, chromosome 18 at 20,528,971 bp
  • C to T, chromosome 18 at 36,676,895 bp
  • T to A, chromosome 18 at 77,404,816 bp
  • A to G, chromosome 19 at 6,372,338 bp
  • T to G, chromosome 19 at 53,851,313 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8100 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067532-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.