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Strain Name:
C57BL/6J-MtgxR8109Btlr/Mmmh
Stock Number:
067538-MU
Citation ID:
RRID:MMRRC_067538-MU
Other Names:
R8109 (G1)
Major Collection:

Strain Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Llgl2
Name: LLGL2 scribble cell polarity complex component
Synonyms: 9130006H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217325
HGNC: HGNC:6629
Homologene: 3323
Tdg
Name: thymine DNA glycosylase
Synonyms: Jza1, E130317C12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21665
Homologene: 2415
Thrap3
Name: thyroid hormone receptor associated protein 3
Synonyms: 9330151F09Rik, Trap150, B230333E16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230753
Homologene: 31289
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 75,551,804 bp
  • T to A, chromosome 2 at 36,786,606 bp
  • A to T, chromosome 2 at 154,627,900 bp
  • T to C, chromosome 2 at 163,339,286 bp
  • A to G, chromosome 2 at 181,671,500 bp
  • A to T, chromosome 3 at 93,290,427 bp
  • A to T, chromosome 3 at 102,851,602 bp
  • G to A, chromosome 3 at 108,203,301 bp
  • A to G, chromosome 4 at 64,008,763 bp
  • T to C, chromosome 4 at 86,248,069 bp
  • A to T, chromosome 4 at 116,051,843 bp
  • C to A, chromosome 4 at 126,186,480 bp
  • T to A, chromosome 4 at 130,169,330 bp
  • C to T, chromosome 4 at 156,009,838 bp
  • T to C, chromosome 5 at 25,281,384 bp
  • A to C, chromosome 5 at 143,985,530 bp
  • T to C, chromosome 6 at 17,562,237 bp
  • A to G, chromosome 6 at 29,755,523 bp
  • T to C, chromosome 6 at 38,329,179 bp
  • G to T, chromosome 6 at 86,721,426 bp
  • A to G, chromosome 7 at 17,975,318 bp
  • T to C, chromosome 7 at 80,238,833 bp
  • T to A, chromosome 7 at 82,602,279 bp
  • C to T, chromosome 7 at 143,897,466 bp
  • A to T, chromosome 8 at 71,537,346 bp
  • T to C, chromosome 8 at 111,040,652 bp
  • A to G, chromosome 9 at 32,181,854 bp
  • T to C, chromosome 9 at 44,935,483 bp
  • G to A, chromosome 9 at 102,041,023 bp
  • T to A, chromosome 9 at 113,911,520 bp
  • T to C, chromosome 10 at 69,990,318 bp
  • T to A, chromosome 10 at 82,644,519 bp
  • A to G, chromosome 10 at 108,699,659 bp
  • T to C, chromosome 11 at 36,008,310 bp
  • T to C, chromosome 11 at 106,046,778 bp
  • T to C, chromosome 11 at 115,850,793 bp
  • C to T, chromosome 12 at 81,918,819 bp
  • T to C, chromosome 12 at 95,780,559 bp
  • T to C, chromosome 13 at 13,359,406 bp
  • A to C, chromosome 13 at 67,449,444 bp
  • T to A, chromosome 14 at 12,488,975 bp
  • C to A, chromosome 14 at 69,800,631 bp
  • A to G, chromosome 14 at 113,315,340 bp
  • A to C, chromosome 15 at 11,331,791 bp
  • G to A, chromosome 15 at 12,373,506 bp
  • T to C, chromosome 17 at 20,815,006 bp
  • A to G, chromosome 17 at 23,644,794 bp
  • A to G, chromosome 18 at 38,198,996 bp
  • T to C, chromosome 18 at 80,117,443 bp
  • T to A, chromosome 19 at 28,964,491 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8109 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067538-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.