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Strain Name:
C57BL/6J-MtgxR8113Btlr/Mmmh
Stock Number:
067542-MU
Citation ID:
RRID:MMRRC_067542-MU
Other Names:
R8113 (G1)
Major Collection:

Strain Information

Ppp5c
Name: protein phosphatase 5, catalytic subunit
Synonyms: ANP receptor, PP5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19060
HGNC: HGNC:9322
Homologene: 4550
Ippk
Name: inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Synonyms: 1810043M15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75678
VEGA: 13
Homologene: 41495
Pzp2
Name: PZP alpha-2-macroglobulin like 2
Synonyms: Pzp
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Sema3f
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Synonyms: Sema IV, Semak
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20350
Homologene: 20885
Lrsam1
Name: leucine rich repeat and sterile alpha motif containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227738
Homologene: 44526
Gmps
Name: guanine monophosphate synthetase
Synonyms: Gm9479
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229363
HGNC: HGNC:4378
Homologene: 68367
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 86,356,642 bp
  • T to G, chromosome 1 at 90,603,603 bp
  • T to A, chromosome 1 at 93,417,195 bp
  • A to C, chromosome 1 at 150,749,090 bp
  • T to A, chromosome 1 at 171,397,518 bp
  • A to G, chromosome 2 at 25,901,211 bp
  • A to G, chromosome 2 at 32,947,889 bp
  • A to G, chromosome 2 at 34,704,318 bp
  • A to T, chromosome 2 at 76,760,049 bp
  • A to G, chromosome 2 at 85,799,667 bp
  • T to G, chromosome 2 at 86,483,068 bp
  • G to A, chromosome 2 at 120,704,430 bp
  • C to G, chromosome 2 at 125,477,569 bp
  • T to C, chromosome 2 at 127,431,347 bp
  • C to A, chromosome 2 at 148,709,431 bp
  • A to G, chromosome 2 at 155,834,820 bp
  • T to C, chromosome 3 at 63,980,269 bp
  • T to A, chromosome 3 at 90,341,722 bp
  • G to A, chromosome 4 at 115,110,066 bp
  • T to C, chromosome 4 at 119,373,746 bp
  • T to A, chromosome 4 at 155,570,670 bp
  • A to G, chromosome 4 at 156,351,347 bp
  • T to C, chromosome 5 at 34,301,419 bp
  • G to A, chromosome 5 at 151,063,505 bp
  • T to TCCC, chromosome 6 at 4,756,451 bp
  • A to T, chromosome 6 at 22,233,481 bp
  • T to C, chromosome 6 at 28,829,903 bp
  • C to T, chromosome 6 at 40,579,427 bp
  • G to A, chromosome 6 at 43,306,017 bp
  • C to T, chromosome 6 at 70,435,103 bp
  • G to T, chromosome 6 at 87,488,305 bp
  • C to T, chromosome 6 at 128,513,731 bp
  • A to G, chromosome 7 at 4,394,115 bp
  • T to C, chromosome 7 at 17,009,007 bp
  • T to C, chromosome 7 at 18,723,062 bp
  • T to C, chromosome 7 at 19,350,177 bp
  • C to T, chromosome 7 at 20,019,335 bp
  • A to G, chromosome 7 at 107,192,508 bp
  • G to T, chromosome 7 at 108,755,231 bp
  • G to T, chromosome 8 at 9,973,485 bp
  • T to A, chromosome 8 at 70,108,571 bp
  • T to C, chromosome 8 at 88,321,803 bp
  • A to T, chromosome 8 at 125,088,275 bp
  • C to T, chromosome 9 at 9,011,433 bp
  • C to T, chromosome 9 at 50,808,780 bp
  • T to A, chromosome 9 at 54,027,137 bp
  • A to G, chromosome 9 at 63,454,573 bp
  • A to T, chromosome 9 at 107,688,076 bp
  • C to A, chromosome 10 at 61,613,971 bp
  • T to C, chromosome 11 at 54,625,958 bp
  • A to T, chromosome 12 at 75,716,600 bp
  • T to C, chromosome 12 at 88,177,080 bp
  • T to A, chromosome 13 at 21,133,201 bp
  • C to T, chromosome 13 at 49,446,342 bp
  • G to A, chromosome 13 at 49,786,072 bp
  • T to G, chromosome 13 at 55,245,621 bp
  • T to G, chromosome 14 at 15,358,232 bp
  • G to A, chromosome 14 at 34,397,636 bp
  • C to A, chromosome 14 at 65,202,813 bp
  • T to C, chromosome 14 at 121,275,596 bp
  • C to T, chromosome 15 at 77,750,136 bp
  • C to T, chromosome 15 at 84,693,792 bp
  • T to C, chromosome 16 at 45,142,188 bp
  • A to T, chromosome 17 at 13,948,976 bp
  • T to A, chromosome 17 at 20,554,467 bp
  • T to C, chromosome 17 at 22,455,500 bp
  • C to G, chromosome 17 at 23,743,802 bp
  • T to C, chromosome 17 at 34,611,612 bp
  • T to A, chromosome 17 at 55,995,881 bp
  • A to T, chromosome 18 at 5,062,385 bp
  • T to C, chromosome 18 at 36,021,103 bp
  • A to T, chromosome 18 at 89,010,916 bp
  • T to A, chromosome 19 at 4,085,582 bp
  • A to C, chromosome 19 at 8,605,539 bp
  • G to T, chromosome 19 at 29,615,796 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8113 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067542-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.