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Strain Name:
C57BL/6J-MtgxR8125Btlr/Mmmh
Stock Number:
067554-MU
Citation ID:
RRID:MMRRC_067554-MU
Other Names:
R8125 (G1)
Major Collection:

Strain Information

Trim32
Name: tripartite motif-containing 32
Synonyms: 3f3, 1810045E12Rik, Zfp117, BBS11
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69807
Homologene: 36327
Slc6a6
Name: solute carrier family 6 (neurotransmitter transporter, taurine), member 6
Synonyms: Taut
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21366
Homologene: 2291
Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Ncoa7
Name: nuclear receptor coactivator 7
Synonyms: 9030406N13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 211329
VEGA: 10
Homologene: 65245
Pibf1
Name: progesterone immunomodulatory binding factor 1
Synonyms: 1700017E21Rik, 4933438D16Rik, 4933439E17Rik, 4930513H15Rik, D14Ertd581e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 52023
VEGA: 14
Homologene: 4628
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, 4930432B04Rik, D730027C18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75786
Homologene: 8844
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 61,767,984 bp
  • T to G, chromosome 1 at 83,263,582 bp
  • C to T, chromosome 1 at 86,024,353 bp
  • T to C, chromosome 1 at 135,403,340 bp
  • C to A, chromosome 1 at 182,747,270 bp
  • C to T, chromosome 2 at 38,701,981 bp
  • C to A, chromosome 2 at 67,512,035 bp
  • T to A, chromosome 2 at 91,576,269 bp
  • T to C, chromosome 2 at 111,379,741 bp
  • A to T, chromosome 2 at 111,499,987 bp
  • T to A, chromosome 3 at 40,709,143 bp
  • T to A, chromosome 3 at 80,707,243 bp
  • T to A, chromosome 3 at 90,065,511 bp
  • T to A, chromosome 3 at 117,837,245 bp
  • A to G, chromosome 4 at 42,873,051 bp
  • T to C, chromosome 4 at 65,613,962 bp
  • C to T, chromosome 4 at 106,760,080 bp
  • T to A, chromosome 5 at 72,358,463 bp
  • A to T, chromosome 5 at 104,815,303 bp
  • G to A, chromosome 5 at 124,579,099 bp
  • C to A, chromosome 6 at 91,726,106 bp
  • T to C, chromosome 6 at 120,224,593 bp
  • T to G, chromosome 6 at 136,401,186 bp
  • A to G, chromosome 7 at 79,729,851 bp
  • A to G, chromosome 7 at 82,450,333 bp
  • T to C, chromosome 7 at 105,764,882 bp
  • A to G, chromosome 7 at 131,099,223 bp
  • A to G, chromosome 8 at 13,577,801 bp
  • A to T, chromosome 8 at 40,794,936 bp
  • C to T, chromosome 8 at 61,983,911 bp
  • G to A, chromosome 8 at 95,455,947 bp
  • A to T, chromosome 8 at 104,292,711 bp
  • T to C, chromosome 9 at 51,826,909 bp
  • T to G, chromosome 9 at 108,487,523 bp
  • T to C, chromosome 10 at 29,196,898 bp
  • T to C, chromosome 10 at 30,694,091 bp
  • T to A, chromosome 10 at 86,820,993 bp
  • C to A, chromosome 10 at 109,852,659 bp
  • A to G, chromosome 10 at 125,328,333 bp
  • C to T, chromosome 11 at 8,947,241 bp
  • T to C, chromosome 11 at 44,972,915 bp
  • A to G, chromosome 11 at 67,299,772 bp
  • A to G, chromosome 11 at 86,186,991 bp
  • T to C, chromosome 11 at 100,463,985 bp
  • T to C, chromosome 11 at 101,460,203 bp
  • TACAAGACAA to TACAA, chromosome 11 at 115,578,237 bp
  • T to A, chromosome 11 at 116,649,290 bp
  • T to A, chromosome 12 at 4,775,487 bp
  • G to A, chromosome 12 at 8,790,663 bp
  • C to A, chromosome 12 at 33,398,314 bp
  • C to T, chromosome 12 at 85,296,612 bp
  • C to T, chromosome 12 at 108,681,524 bp
  • T to C, chromosome 13 at 76,130,327 bp
  • A to T, chromosome 13 at 81,440,225 bp
  • A to T, chromosome 13 at 92,299,182 bp
  • C to T, chromosome 14 at 99,179,367 bp
  • A to G, chromosome 15 at 92,743,595 bp
  • A to T, chromosome 16 at 88,896,348 bp
  • T to C, chromosome 17 at 26,124,620 bp
  • G to A, chromosome 17 at 28,550,728 bp
  • A to T, chromosome 17 at 35,065,352 bp
  • A to T, chromosome 17 at 37,280,044 bp
  • T to C, chromosome 17 at 45,539,692 bp
  • C to G, chromosome 17 at 71,716,494 bp
  • A to G, chromosome 17 at 86,922,367 bp
  • C to A, chromosome 18 at 24,475,652 bp
  • G to A, chromosome 18 at 61,707,505 bp
  • C to A, chromosome 18 at 76,859,678 bp
  • A to T, chromosome 18 at 84,561,185 bp
  • C to T, chromosome 19 at 9,011,876 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8125 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067554-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.