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Strain Name:
C57BL/6J-MtgxR8139Btlr/Mmmh
Stock Number:
067567-MU
Citation ID:
RRID:MMRRC_067567-MU
Other Names:
R8139 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Vps33a
Name: VPS33A CORVET/HOPS core subunit
Synonyms: bf, 3830421M04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77573
Homologene: 11294
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Tmed10
Name: transmembrane p24 trafficking protein 10
Synonyms: 1110014C03Rik, p24delta1, p23
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68581
VEGA: 12
Homologene: 4972
Xpnpep3
Name: X-prolyl aminopeptidase 3, mitochondrial
Synonyms: E430012M05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 321003
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,191,852 bp
  • A to T, chromosome 1 at 82,289,739 bp
  • A to G, chromosome 1 at 134,300,528 bp
  • A to T, chromosome 1 at 180,069,319 bp
  • A to G, chromosome 1 at 181,054,793 bp
  • G to T, chromosome 1 at 181,755,288 bp
  • G to A, chromosome 2 at 74,876,370 bp
  • A to G, chromosome 2 at 89,097,843 bp
  • A to C, chromosome 2 at 142,901,365 bp
  • C to T, chromosome 2 at 155,814,045 bp
  • A to G, chromosome 3 at 84,451,483 bp
  • G to T, chromosome 3 at 97,696,993 bp
  • A to G, chromosome 3 at 105,927,998 bp
  • A to G, chromosome 3 at 114,097,049 bp
  • G to T, chromosome 3 at 121,455,069 bp
  • A to G, chromosome 3 at 124,321,787 bp
  • G to A, chromosome 5 at 43,758,745 bp
  • T to C, chromosome 5 at 75,652,805 bp
  • A to G, chromosome 5 at 99,924,926 bp
  • T to C, chromosome 5 at 123,533,952 bp
  • C to T, chromosome 5 at 124,113,475 bp
  • T to A, chromosome 5 at 135,636,335 bp
  • A to T, chromosome 6 at 24,522,758 bp
  • C to T, chromosome 6 at 48,439,334 bp
  • T to C, chromosome 6 at 142,089,900 bp
  • G to T, chromosome 7 at 12,615,816 bp
  • T to C, chromosome 7 at 16,123,976 bp
  • T to C, chromosome 7 at 43,463,729 bp
  • T to C, chromosome 7 at 97,565,243 bp
  • T to A, chromosome 7 at 108,322,906 bp
  • A to G, chromosome 7 at 110,899,672 bp
  • T to C, chromosome 7 at 119,720,585 bp
  • A to G, chromosome 7 at 123,269,976 bp
  • T to C, chromosome 7 at 142,438,268 bp
  • A to G, chromosome 7 at 144,520,693 bp
  • A to T, chromosome 8 at 84,702,847 bp
  • T to A, chromosome 8 at 95,558,738 bp
  • A to T, chromosome 9 at 8,956,340 bp
  • A to T, chromosome 9 at 19,035,575 bp
  • A to C, chromosome 9 at 19,361,208 bp
  • A to T, chromosome 9 at 44,819,290 bp
  • A to T, chromosome 9 at 97,080,452 bp
  • T to A, chromosome 9 at 103,499,885 bp
  • T to C, chromosome 10 at 40,257,473 bp
  • C to A, chromosome 10 at 69,266,290 bp
  • A to G, chromosome 10 at 115,839,897 bp
  • G to A, chromosome 10 at 117,004,426 bp
  • T to C, chromosome 11 at 64,980,614 bp
  • A to G, chromosome 11 at 102,867,859 bp
  • T to A, chromosome 11 at 110,184,133 bp
  • A to G, chromosome 12 at 85,344,317 bp
  • A to G, chromosome 12 at 90,204,664 bp
  • G to T, chromosome 12 at 112,601,640 bp
  • C to A, chromosome 14 at 32,092,091 bp
  • T to C, chromosome 14 at 44,858,685 bp
  • T to A, chromosome 15 at 35,607,272 bp
  • T to C, chromosome 15 at 76,174,310 bp
  • T to A, chromosome 15 at 81,448,459 bp
  • C to T, chromosome 15 at 82,178,765 bp
  • A to T, chromosome 17 at 25,383,723 bp
  • G to T, chromosome 17 at 34,584,800 bp
  • T to C, chromosome 17 at 35,011,504 bp
  • C to T, chromosome 17 at 56,767,058 bp
  • C to A, chromosome 17 at 83,631,087 bp
  • T to C, chromosome 18 at 5,214,013 bp
  • A to G, chromosome 18 at 34,473,633 bp
  • A to G, chromosome 18 at 36,962,738 bp
  • G to A, chromosome 18 at 77,380,496 bp
  • T to C, chromosome 18 at 84,951,138 bp
  • A to T, chromosome 19 at 5,665,745 bp
  • T to G, chromosome 19 at 6,119,915 bp
  • T to C, chromosome 19 at 6,119,916 bp
  • T to C, chromosome 19 at 11,667,754 bp
  • G to T, chromosome X at 70,394,514 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8139 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067567-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.