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Strain Name:
C57BL/6J-MtgxR8140Btlr/Mmmh
Stock Number:
067568-MU
Citation ID:
RRID:MMRRC_067568-MU
Other Names:
R8140 (G1)
Major Collection:

Strain Information

Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Zfand6
Name: zinc finger, AN1-type domain 6
Synonyms: Awp1, 3110005P07Rik, Za20d3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 65098
Homologene: 10372
Pipox
Name: pipecolic acid oxidase
Synonyms: Pso
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19193
Homologene: 40640
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15184
Homologene: 3995
Slc37a1
Name: solute carrier family 37 (glycerol-3-phosphate transporter), member 1
Synonyms: G3PP
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224674
VEGA: 17
Homologene: 70486
Mthfd1l
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms: 2410004L15Rik, Fthfsdc1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 270685
Homologene: 56706
Hcrtr1
Name: hypocretin (orexin) receptor 1
Synonyms: OX1R
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230777
HGNC: HGNC:4848
Homologene: 37492
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,325,294 bp
  • G to T, chromosome 1 at 36,771,181 bp
  • G to A, chromosome 1 at 38,051,276 bp
  • A to T, chromosome 1 at 53,501,589 bp
  • A to T, chromosome 1 at 152,557,501 bp
  • A to G, chromosome 1 at 157,299,235 bp
  • A to G, chromosome 1 at 159,863,695 bp
  • A to T, chromosome 1 at 183,293,873 bp
  • A to G, chromosome 2 at 22,407,346 bp
  • T to A, chromosome 2 at 32,244,297 bp
  • T to C, chromosome 2 at 52,209,540 bp
  • T to C, chromosome 2 at 76,771,651 bp
  • T to C, chromosome 2 at 88,402,113 bp
  • A to T, chromosome 2 at 130,615,250 bp
  • A to G, chromosome 3 at 90,505,393 bp
  • T to C, chromosome 3 at 104,034,086 bp
  • C to T, chromosome 4 at 122,949,298 bp
  • G to A, chromosome 4 at 130,135,290 bp
  • T to A, chromosome 5 at 63,898,611 bp
  • C to G, chromosome 6 at 4,756,113 bp
  • A to T, chromosome 6 at 30,544,905 bp
  • T to C, chromosome 6 at 127,965,278 bp
  • A to G, chromosome 7 at 45,238,535 bp
  • C to T, chromosome 7 at 45,588,351 bp
  • A to T, chromosome 7 at 84,632,749 bp
  • A to C, chromosome 7 at 96,895,176 bp
  • G to T, chromosome 7 at 121,649,026 bp
  • C to A, chromosome 8 at 22,028,560 bp
  • A to T, chromosome 8 at 25,030,558 bp
  • T to C, chromosome 8 at 41,200,609 bp
  • C to T, chromosome 8 at 117,047,497 bp
  • G to A, chromosome 9 at 14,572,715 bp
  • A to T, chromosome 9 at 15,341,533 bp
  • A to G, chromosome 9 at 37,383,871 bp
  • G to T, chromosome 9 at 44,111,972 bp
  • G to T, chromosome 9 at 105,392,957 bp
  • C to T, chromosome 10 at 4,007,745 bp
  • T to A, chromosome 10 at 59,049,355 bp
  • C to T, chromosome 11 at 69,746,376 bp
  • T to C, chromosome 11 at 77,883,909 bp
  • A to T, chromosome 11 at 102,197,355 bp
  • T to A, chromosome 12 at 75,912,353 bp
  • T to A, chromosome 12 at 81,378,151 bp
  • G to A, chromosome 12 at 114,583,741 bp
  • T to C, chromosome 13 at 103,920,294 bp
  • A to G, chromosome 14 at 33,142,360 bp
  • A to G, chromosome 15 at 65,995,541 bp
  • C to A, chromosome 15 at 76,433,873 bp
  • A to C, chromosome 15 at 85,818,410 bp
  • A to G, chromosome 16 at 3,713,635 bp
  • T to C, chromosome 16 at 58,889,585 bp
  • T to A, chromosome 16 at 77,071,681 bp
  • A to T, chromosome 17 at 19,811,796 bp
  • A to T, chromosome 17 at 31,322,259 bp
  • A to G, chromosome 17 at 35,601,774 bp
  • T to G, chromosome 18 at 9,213,797 bp
  • T to C, chromosome 18 at 37,964,342 bp
  • T to A, chromosome 18 at 52,488,690 bp
  • A to T, chromosome 19 at 13,795,239 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8140 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067568-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.