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Strain Name:
C57BL/6J-MtgxR8165Btlr/Mmmh
Stock Number:
067591-MU
Citation ID:
RRID:MMRRC_067591-MU
Other Names:
R8165 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Areg
Name: amphiregulin
Synonyms: AR, Sdgf, Mcub
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11839
HGNC: HGNC:651
Homologene: 1252
Mad1l1
Name: MAD1 mitotic arrest deficient 1-like 1
Synonyms: Mad1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17120
HGNC: HGNC:6762
Homologene: 74500
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Bcar3
Name: breast cancer anti-estrogen resistance 3
Synonyms: AND-34
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 29815
HGNC: HGNC:973
Homologene: 31181
Zfp710
Name: zinc finger protein 710
Synonyms: 5430400N05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 209225
Homologene: 19625
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,308,929 bp
  • A to G, chromosome 1 at 90,612,007 bp
  • G to A, chromosome 1 at 150,646,658 bp
  • C to A, chromosome 1 at 188,451,755 bp
  • A to G, chromosome 2 at 26,946,656 bp
  • T to A, chromosome 2 at 36,421,703 bp
  • A to G, chromosome 2 at 66,540,530 bp
  • C to A, chromosome 2 at 119,947,238 bp
  • T to C, chromosome 2 at 120,370,478 bp
  • T to A, chromosome 2 at 130,280,500 bp
  • T to A, chromosome 2 at 140,262,706 bp
  • A to T, chromosome 2 at 153,420,553 bp
  • G to C, chromosome 3 at 40,813,574 bp
  • C to T, chromosome 3 at 121,988,573 bp
  • C to T, chromosome 3 at 122,511,156 bp
  • T to A, chromosome 3 at 127,563,383 bp
  • T to A, chromosome 4 at 11,542,128 bp
  • C to T, chromosome 4 at 40,270,665 bp
  • C to T, chromosome 4 at 148,944,431 bp
  • C to A, chromosome 5 at 63,897,946 bp
  • A to T, chromosome 5 at 75,620,880 bp
  • A to G, chromosome 5 at 91,143,633 bp
  • A to G, chromosome 5 at 100,454,499 bp
  • T to C, chromosome 5 at 140,315,058 bp
  • T to A, chromosome 6 at 12,468,963 bp
  • A to G, chromosome 6 at 30,564,346 bp
  • C to T, chromosome 6 at 147,631,588 bp
  • T to A, chromosome 7 at 25,353,873 bp
  • T to C, chromosome 7 at 80,086,027 bp
  • A to T, chromosome 7 at 99,456,482 bp
  • G to A, chromosome 7 at 101,500,448 bp
  • A to T, chromosome 7 at 119,450,152 bp
  • T to C, chromosome 7 at 135,528,404 bp
  • A to G, chromosome 8 at 53,589,094 bp
  • A to C, chromosome 8 at 91,041,141 bp
  • C to A, chromosome 8 at 105,620,888 bp
  • T to C, chromosome 8 at 107,083,024 bp
  • A to G, chromosome 9 at 67,858,790 bp
  • A to G, chromosome 9 at 107,525,454 bp
  • T to A, chromosome 9 at 119,066,753 bp
  • C to T, chromosome 10 at 7,154,467 bp
  • T to A, chromosome 10 at 24,027,597 bp
  • A to T, chromosome 10 at 88,677,566 bp
  • A to G, chromosome 11 at 26,535,575 bp
  • T to A, chromosome 11 at 46,441,246 bp
  • A to T, chromosome 11 at 50,210,974 bp
  • A to G, chromosome 11 at 97,351,538 bp
  • C to T, chromosome 11 at 121,493,885 bp
  • C to T, chromosome 12 at 85,303,643 bp
  • T to A, chromosome 13 at 13,698,360 bp
  • A to G, chromosome 13 at 19,094,837 bp
  • T to C, chromosome 14 at 7,994,299 bp
  • A to G, chromosome 14 at 31,138,929 bp
  • A to G, chromosome 15 at 57,960,949 bp
  • T to C, chromosome 16 at 22,987,496 bp
  • G to A, chromosome 16 at 91,616,907 bp
  • T to C, chromosome 17 at 26,937,070 bp
  • C to T, chromosome 17 at 28,806,274 bp
  • T to A, chromosome 17 at 34,368,708 bp
  • A to G, chromosome 17 at 53,515,229 bp
  • G to A, chromosome 17 at 56,107,019 bp
  • T to A, chromosome 18 at 56,589,475 bp
  • A to G, chromosome 19 at 41,088,874 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8165 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067591-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.