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Strain Name:
C57BL/6J-MtgxR8190Btlr/Mmmh
Stock Number:
067613-MU
Citation ID:
RRID:MMRRC_067613-MU
Other Names:
R8190 (G1)
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Htr4
Name: 5 hydroxytryptamine (serotonin) receptor 4
Synonyms: 5-HT4L, 5-HT4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 15562
VEGA: 18
HGNC: HGNC:5299
Homologene: 20243
Gsta4
Name: glutathione S-transferase, alpha 4
Synonyms: GST 5.7, mGsta4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14860
VEGA: 9
Homologene: 135605
Ptpa
Name: protein phosphatase 2 protein activator
Synonyms: 2610042B21Rik, Ppp2r4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110854
HGNC: HGNC:9308
Homologene: 6149
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67414
Homologene: 11481
Slc1a6
Name: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
Synonyms: EAAT4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20513
VEGA: 10
Homologene: 21055
Zfp113
Name: zinc finger protein 113
Synonyms: 4732456B05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56314
Homologene: 50027
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 43,771,751 bp
  • G to T, chromosome 1 at 74,434,893 bp
  • G to A, chromosome 2 at 30,438,339 bp
  • T to A, chromosome 2 at 79,333,557 bp
  • C to T, chromosome 2 at 91,772,352 bp
  • C to T, chromosome 2 at 118,690,097 bp
  • A to T, chromosome 2 at 122,047,488 bp
  • A to G, chromosome 3 at 10,321,628 bp
  • A to T, chromosome 3 at 32,568,389 bp
  • A to T, chromosome 3 at 88,090,779 bp
  • A to T, chromosome 3 at 122,896,770 bp
  • G to A, chromosome 4 at 143,350,733 bp
  • T to G, chromosome 4 at 143,571,960 bp
  • T to A, chromosome 4 at 145,152,751 bp
  • A to T, chromosome 5 at 34,325,158 bp
  • A to T, chromosome 5 at 65,623,998 bp
  • A to T, chromosome 5 at 76,227,204 bp
  • A to C, chromosome 5 at 124,117,032 bp
  • T to A, chromosome 5 at 137,467,084 bp
  • T to A, chromosome 5 at 137,655,402 bp
  • G to T, chromosome 5 at 138,144,996 bp
  • A to T, chromosome 6 at 38,818,793 bp
  • A to G, chromosome 6 at 59,354,471 bp
  • A to G, chromosome 6 at 67,036,829 bp
  • C to T, chromosome 6 at 88,222,958 bp
  • C to A, chromosome 7 at 4,471,298 bp
  • A to G, chromosome 7 at 10,714,392 bp
  • T to C, chromosome 7 at 23,754,143 bp
  • T to C, chromosome 7 at 25,666,344 bp
  • A to T, chromosome 7 at 67,733,906 bp
  • A to G, chromosome 7 at 79,723,280 bp
  • A to T, chromosome 7 at 103,777,595 bp
  • A to G, chromosome 7 at 108,211,807 bp
  • G to T, chromosome 7 at 139,876,118 bp
  • T to C, chromosome 7 at 141,314,411 bp
  • A to G, chromosome 8 at 88,311,038 bp
  • T to C, chromosome 9 at 22,678,988 bp
  • T to A, chromosome 9 at 66,418,451 bp
  • T to C, chromosome 9 at 78,198,372 bp
  • T to G, chromosome 9 at 98,444,656 bp
  • T to A, chromosome 9 at 110,202,534 bp
  • C to T, chromosome 9 at 110,626,090 bp
  • A to G, chromosome 10 at 78,791,233 bp
  • T to A, chromosome 10 at 81,638,318 bp
  • C to T, chromosome 10 at 88,972,745 bp
  • C to T, chromosome 10 at 117,074,879 bp
  • A to G, chromosome 11 at 49,216,075 bp
  • C to A, chromosome 11 at 65,038,653 bp
  • T to A, chromosome 11 at 74,054,479 bp
  • T to G, chromosome 11 at 82,897,860 bp
  • T to G, chromosome 11 at 117,791,360 bp
  • T to C, chromosome 12 at 16,549,002 bp
  • T to C, chromosome 12 at 65,006,912 bp
  • T to C, chromosome 12 at 79,280,836 bp
  • T to A, chromosome 12 at 101,757,296 bp
  • T to C, chromosome 13 at 105,053,109 bp
  • T to C, chromosome 14 at 29,984,073 bp
  • T to A, chromosome 14 at 46,384,515 bp
  • C to A, chromosome 14 at 47,530,422 bp
  • A to T, chromosome 14 at 50,345,722 bp
  • T to C, chromosome 14 at 55,512,028 bp
  • C to T, chromosome 14 at 55,704,884 bp
  • T to A, chromosome 14 at 123,599,939 bp
  • A to T, chromosome 15 at 77,717,807 bp
  • A to G, chromosome 15 at 85,902,889 bp
  • A to T, chromosome 16 at 21,575,030 bp
  • A to T, chromosome 16 at 22,961,043 bp
  • C to T, chromosome 17 at 27,986,804 bp
  • C to T, chromosome 17 at 52,956,908 bp
  • G to T, chromosome 17 at 74,493,972 bp
  • G to A, chromosome 18 at 39,775,467 bp
  • A to G, chromosome 18 at 62,437,900 bp
  • G to A, chromosome 19 at 9,002,255 bp
  • G to T, chromosome 19 at 46,401,197 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8190 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067613-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.