Strain Name:
C57BL/6J-MtgxR8204Btlr/Mmmh
Stock Number:
067627-MU
Citation ID:
RRID:MMRRC_067627-MU
Other Names:
R8204 (G1)
Major Collection:

Strain Information

Cdk6
Name: cyclin dependent kinase 6
Synonyms: Crk2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12571
HGNC: HGNC:1777
Homologene: 963
Xrcc1
Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Synonyms: Xrcc-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22594
Homologene: 31368
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: ARPP-21, D9Bwg1012e, Tarpp, R3hdm3, 0710001E13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74100
Homologene: 32306
Exoc6b
Name: exocyst complex component 6B
Synonyms: G430127E12Rik, 4930569O18Rik, Sec15b, Sec15l2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75914
Homologene: 44781
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: D5Ertd66e, Bwf1, 2610509D04Rik, Bchs, Ggtb3, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57752
Homologene: 5087
Tcof1
Name: treacle ribosome biogenesis factor 1
Synonyms: treacle
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 21453
Homologene: 68049
Git1
Name: GIT ArfGAP 1
Synonyms: p95Cat, Cat-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216963
HGNC: HGNC:4272
Homologene: 32204
Ppp2r1a
Name: protein phosphatase 2, regulatory subunit A, alpha
Synonyms: PP2A, protein phosphatase PP2A, PR65, 6330556D22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 51792
HGNC: HGNC:9302
Homologene: 68559
Tfcp2
Name: transcription factor CP2
Synonyms: LBP-1c, Tcfcp2, D230015P20Rik, UBP-1, CP-2, LBP-1d, LSF, LBP1, CP2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 21422
VEGA: 15
Homologene: 4134
Ppp1r7
Name: protein phosphatase 1, regulatory subunit 7
Synonyms: 2310014J01Rik, SDS22
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66385
HGNC: HGNC:9295
Homologene: 2032
Hk1
Name: hexokinase 1
Synonyms: Hk-1, mHk1-s, Hk1-s
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15275
HGNC: HGNC:4922
Homologene: 100530
Magi3
Name: membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms: 4732496O19Rik, 6530407C02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99470
Homologene: 26431
Tcerg1
Name: transcription elongation regulator 1 (CA150)
Synonyms: ca150, Fbp28, 2900090C16Rik, 2410022J09Rik, p144, Taf2s
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 56070
Homologene: 4879
Katnip
Name: katanin interacting protein
Synonyms: D430042O09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233865
Homologene: 45841
Klhl20
Name: kelch-like 20
Synonyms: D930050H05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226541
Homologene: 8699
Clec4d
Name: C-type lectin domain family 4, member d
Synonyms: mMCL, Mpcl, Clecsf8, mcl
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17474
Homologene: 7844
Trpc7
Name: transient receptor potential cation channel, subfamily C, member 7
Synonyms: TRP7, Trrp8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26946
Homologene: 22689
Egln3
Name: egl-9 family hypoxia-inducible factor 3
Synonyms: Hif-p4h-3, 2610021G09Rik, SM-20, Phd3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 112407
Homologene: 32531
Fat2
Name: FAT atypical cadherin 2
Synonyms: EMI2, mKIAA0811, LOC245827, Fath2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Itga9
Name: integrin alpha 9
Synonyms: 6720458D17Rik, 2610002H11Rik, D9Ertd428e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 104099
HGNC: HGNC:6145
Homologene: 1664
Mast3
Name: microtubule associated serine/threonine kinase 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 546071
Homologene: 66191
BC034090
Name: cDNA sequence BC034090
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 207792
Homologene: 19490
Cox8a
Name: cytochrome c oxidase subunit 8A
Synonyms: COX VIII-L, COX8L
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12868
VEGA: 19
HGNC: HGNC:2294
Homologene: 3006
Cep126
Name: centrosomal protein 126
Synonyms: AK129341
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 234915
VEGA: 9
Homologene: 28313
Flg2
Name: filaggrin family member 2
Synonyms: EG229574
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229574
Homologene: 134146
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50720
4933427D14Rik
Name: RIKEN cDNA 4933427D14 gene
Synonyms: Gm43951
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74477
Homologene: 8874
Dlg5
Name: discs large MAGUK scaffold protein 5
Synonyms: 4933429D20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71228
HGNC: HGNC:2904
Homologene: 3486
Or5af2
Name: olfactory receptor family 5 subfamily AC member 20
Synonyms: Olfr313, GA_x6K02T2NKPP-590035-589109, MOR222-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258529
Homologene: 77375
Or4f54
Name: olfactory receptor family 4 subfamily F member 54
Synonyms: MOR245-11, Olfr1278, GA_x6K02T2Q125-72343713-72344654
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258389
Homologene: 74061
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 639774
Homologene: 106613
Lancl2
Name: LanC (bacterial lantibiotic synthetase component C)-like 2
Synonyms: 1700003F10Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71835
HGNC: HGNC:6509
Homologene: 23116
Gpr137
Name: G protein-coupled receptor 137
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107173
VEGA: 19
Homologene: 10598
Pyroxd2
Name: pyridine nucleotide-disulphide oxidoreductase domain 2
Synonyms: 4833409A17Rik, 3830409H07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74580
VEGA: 19
Homologene: 13097
Plekhg6
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 6
Synonyms: LOC213522
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 213522
Homologene: 32395
Prkag2
Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Synonyms: 2410051C13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108099
HGNC: HGNC:9386
Homologene: 136125
Lrtm2
Name: leucine-rich repeats and transmembrane domains 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211187
Homologene: 65244
Myrip
Name: myosin VIIA and Rab interacting protein
Synonyms: A230081N12Rik, Slac2-c
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245049
Homologene: 9150
Tpp1
Name: tripeptidyl peptidase I
Synonyms: Cln2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12751
HGNC: HGNC:2073
Homologene: 335
Sh3tc2
Name: SH3 domain and tetratricopeptide repeats 2
Synonyms: D430044G18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225608
VEGA: 18
Homologene: 11596
Cep20
Name: centrosomal protein 20
Synonyms: Fopnl, 0610037P05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66086
Homologene: 11919
Hoxc13
Name: homeobox C13
Synonyms: N
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 15422
HGNC: HGNC:5125
Homologene: 7770
Vmn1r55
Name: vomeronasal 1 receptor 55
Synonyms: LOC384522, V1rd5, LOC236535
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384522
Homologene: 41799
Myl10
Name: myosin, light chain 10, regulatory
Synonyms: PLRLC-C, 1700027I08Rik, Mylc2pl, PLRLC, PLRLC-A, PLRLC-B
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 59310
Homologene: 69341
Mfsd7c
Name:
Type: Gene
Species: Mouse
Chromosome: 12
Krtap19-9a
Name: keratin associated protein 19-9A
Synonyms: Gm9829, Krtap16-10a
Type: Gene
Species: Mouse
Chromosome: 16
Adrbk2
Name:
Type: Gene
Species: Mouse
Chromosome: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 93,365,011 bp
  • C to A, chromosome 1 at 155,241,742 bp
  • T to C, chromosome 1 at 161,106,844 bp
  • A to G, chromosome 2 at 111,293,140 bp
  • T to C, chromosome 3 at 86,315,403 bp
  • T to A, chromosome 3 at 93,202,767 bp
  • A to T, chromosome 3 at 104,051,186 bp
  • T to C, chromosome 4 at 111,938,893 bp
  • A to G, chromosome 5 at 3,344,461 bp
  • T to C, chromosome 5 at 24,869,127 bp
  • A to G, chromosome 5 at 101,852,585 bp
  • A to G, chromosome 5 at 112,957,359 bp
  • G to C, chromosome 5 at 136,697,971 bp
  • C to A, chromosome 6 at 57,737,716 bp
  • C to T, chromosome 6 at 84,855,522 bp
  • T to A, chromosome 6 at 119,317,408 bp
  • T to A, chromosome 6 at 123,265,364 bp
  • C to T, chromosome 6 at 125,363,498 bp
  • A to T, chromosome 7 at 5,147,286 bp
  • T to C, chromosome 7 at 24,572,284 bp
  • A to T, chromosome 7 at 105,750,315 bp
  • C to T, chromosome 7 at 125,850,742 bp
  • A to T, chromosome 7 at 130,624,429 bp
  • T to C, chromosome 8 at 70,788,281 bp
  • C to T, chromosome 9 at 8,120,780 bp
  • T to C, chromosome 9 at 95,935,513 bp
  • T to A, chromosome 9 at 112,136,570 bp
  • A to G, chromosome 9 at 118,871,921 bp
  • G to A, chromosome 9 at 120,432,979 bp
  • A to G, chromosome 10 at 62,296,744 bp
  • A to T, chromosome 11 at 55,284,610 bp
  • A to C, chromosome 11 at 58,817,059 bp
  • C to T, chromosome 11 at 72,166,780 bp
  • G to A, chromosome 11 at 77,505,335 bp
  • T to C, chromosome 12 at 54,203,224 bp
  • GTAGTGTATA to GTA, chromosome 12 at 85,803,148 bp
  • A to G, chromosome 13 at 56,783,796 bp
  • A to G, chromosome 14 at 24,160,252 bp
  • T to C, chromosome 14 at 61,212,948 bp
  • G to T, chromosome 15 at 100,522,448 bp
  • A to G, chromosome 15 at 102,927,360 bp
  • T to C, chromosome 16 at 14,300,206 bp
  • C to T, chromosome 16 at 88,924,108 bp
  • A to G, chromosome 17 at 20,956,773 bp
  • T to A, chromosome 18 at 42,574,553 bp
  • G to C, chromosome 18 at 60,829,051 bp
  • T to C, chromosome 18 at 61,953,129 bp
  • C to T, chromosome 19 at 6,940,378 bp
  • A to G, chromosome 19 at 7,215,480 bp
  • A to T, chromosome 19 at 42,749,388 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8204 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067627-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.