Strain Name:
C57BL/6J-MtgxR8218Btlr/Mmmh
Stock Number:
067637-MU
Citation ID:
RRID:MMRRC_067637-MU
Other Names:
R8218 (G1)
Major Collection:

Strain Information

Prkar2a
Name: protein kinase, cAMP dependent regulatory, type II alpha
Synonyms: RII(alpha), 1110061A24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19087
HGNC: HGNC:9391
Homologene: 3064
Kcnj10
Name: potassium inwardly-rectifying channel, subfamily J, member 10
Synonyms: Kir1.2, BIRK-1, BIR10, Kir4.1, Kir4.1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16513
HGNC: HGNC:6256
Homologene: 1689
Ubn2
Name: ubinuclein 2
Synonyms: 6030408G03Rik, 2900060J04Rik, D130059P03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320538
Homologene: 45564
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: 1100001C18Rik, A330076K04Rik, 2500002E12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70097
Homologene: 69182
Eif4g2
Name: eukaryotic translation initiation factor 4, gamma 2
Synonyms: Nat1, DAP-5, Natm1, E130105L11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13690
HGNC: HGNC:3297
Homologene: 37477
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: GCN1L, G431004K08Rik, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Exo1
Name: exonuclease 1
Synonyms: Msa, 5730442G03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26909
HGNC: HGNC:3511
Homologene: 31352
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Pip5k1c
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma
Synonyms: PIP5KIgamma
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18717
HGNC: HGNC:8996
Homologene: 69032
Tlr2
Name: toll-like receptor 2
Synonyms: Ly105
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 24088
Homologene: 20695
Esr1
Name: estrogen receptor 1 (alpha)
Synonyms: ESR, ERalpha, ER[a], Nr3a1, ERa, Estr, Estra, ER-alpha
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13982
HGNC: HGNC:3467
Homologene: 47906
Fam83h
Name: family with sequence similarity 83, member H
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105732
VEGA: 15
Homologene: 15890
Aff4
Name: AF4/FMR2 family, member 4
Synonyms: Alf4, Laf4l
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 93736
Homologene: 8683
Rasal2
Name: RAS protein activator like 2
Synonyms: A330066M24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226525
HGNC: HGNC:9874
Homologene: 35217
Tsn
Name: translin
Synonyms: TB-RBP, 2610034C24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22099
Homologene: 3397
Iws1
Name: IWS1, SUPT6 interacting protein
Synonyms: 1700069O15Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 73473
Homologene: 134421
Robo1
Name: roundabout guidance receptor 1
Synonyms: DUTT1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19876
Homologene: 2206
Slc39a8
Name: solute carrier family 39 (metal ion transporter), member 8
Synonyms: BIGM103, 4933419D20Rik, ZIP8
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67547
Homologene: 11155
Acp5
Name: acid phosphatase 5, tartrate resistant
Synonyms: TRAP, TRACP
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11433
VEGA: 9
HGNC: HGNC:124
Homologene: 137203
Pcdh20
Name: protocadherin 20
Synonyms: PCDH13, C630015B17Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219257
Homologene: 11277
C1qtnf2
Name: C1q and tumor necrosis factor related protein 2
Synonyms: 1810033K05Rik, CTRP2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69183
Homologene: 12899
Muc16
Name: mucin 16
Synonyms: LOC385009, 1110008I14Rik, Gm21044
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 73732
Homologene: 141193
Yy1
Name: YY1 transcription factor
Synonyms: UCRBP transcription factor, delta transcription factor, NF-E1, Yin Yang 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22632
Homologene: 2556
Dctn2
Name: dynactin 2
Synonyms: RBP50, DCTN-50, p50, 2310042E05Rik, C130077D06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 69654
VEGA: 10
HGNC: HGNC:2712
Homologene: 4667
Atp5pb
Name: ATP synthase peripheral stalk-membrane subunit b
Synonyms: C76477, Atp5f1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11950
HGNC: HGNC:840
Homologene: 1275
Rbm4
Name: RNA binding motif protein 4
Synonyms: Lark1, Rbm4a
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19653
HGNC: HGNC:9901
Homologene: 82510
Tube1
Name: tubulin, epsilon 1
Synonyms: 2310061K05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71924
VEGA: 10
Homologene: 17762
Wdr75
Name: WD repeat domain 75
Synonyms: 1300003A18Rik, 2410118I19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73674
Homologene: 32771
Qser1
Name: glutamine and serine rich 1
Synonyms: 4732486I23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99003
Homologene: 11710
Ehbp1
Name: EH domain binding protein 1
Synonyms: Flj21950, KIAA0903-like
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216565
Homologene: 22880
Ackr4
Name: atypical chemokine receptor 4
Synonyms: VSHK1, CCR11, CCBP2, PPR1, CCX-CKR, A630091E18Rik, Ccrl1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 252837
HGNC: HGNC:1611
Homologene: 49474
Sycp2l
Name: synaptonemal complex protein 2-like
Synonyms: LOC218175, EG621792, Gm40956
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 637277
Homologene: 52882
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26875
Homologene: 69111
Slc22a4
Name: solute carrier family 22 (organic cation transporter), member 4
Synonyms: Octn1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 30805
Homologene: 81701
Iqch
Name: IQ motif containing H
Synonyms: 4921504K03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 78250
Homologene: 11258
Pter
Name: phosphotriesterase related
Synonyms: Mpr56-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19212
HGNC: HGNC:9590
Homologene: 7495
Ppfia2
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Synonyms: E130120L08Rik, Liprin-alpha2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 327814
VEGA: 10
HGNC: HGNC:9246
Homologene: 27953
Syt11
Name: synaptotagmin XI
Synonyms: 6530420C11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229521
Homologene: 23120
Skint6
Name: selection and upkeep of intraepithelial T cells 6
Synonyms: OTTMUSG00000008519
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230622
Homologene: 135888
Pik3ca
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms: p110alpha, caPI3K, 6330412C24Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18706
HGNC: HGNC:8975
Homologene: 21249
Kcnh6
Name: potassium voltage-gated channel, subfamily H (eag-related), member 6
Synonyms: m-erg2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192775
Homologene: 32740
Or1j17
Name: olfactory receptor family 1 subfamily J member 17
Synonyms: GA_x6K02T2NLDC-33382467-33383396, MOR136-11, Olfr346
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258940
Homologene: 133616
Grk4
Name: G protein-coupled receptor kinase 4
Synonyms: A830025H08Rik, Gprk2l
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14772
HGNC: HGNC:4543
Homologene: 23158
Vmn2r108
Name: vomeronasal 2, receptor 108
Synonyms: EG627805
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627805
Homologene: 129678
Dennd3
Name: DENN domain containing 3
Synonyms: E030003N15Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105841
Homologene: 28254
Atp8b5
Name: ATPase, class I, type 8B, member 5
Synonyms: FetA, 4930417M19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320571
Homologene: 99882
Gstp2
Name: glutathione S-transferase, pi 2
Synonyms: Gst-3, Gst3, Gst p-2, GSTpiA
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14869
HGNC: HGNC:4638
Homologene: 660
Phf2
Name: PHD finger protein 2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18676
VEGA: 13
HGNC: HGNC:8920
Homologene: 3934
5031439G07Rik
Name: RIKEN cDNA 5031439G07 gene
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223739
HGNC: HGNC:1314
Homologene: 15140
Svopl
Name: SV2 related protein homolog (rat)-like
Synonyms: 9430071P14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320590
Homologene: 52168
Pidd1
Name: p53 induced death domain protein 1
Synonyms: 1200011D09Rik, Pidd, Lrdd
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57913
Homologene: 11220
Syt5
Name: synaptotagmin V
Synonyms: SytIX
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53420
Homologene: 55722
Slc40a1
Name: solute carrier family 40 (iron-regulated transporter), member 1
Synonyms: Ol5, ferroportin1, IREG1, FPN1, metal transporting protein 1, MTP1, Dusg, Slc11a3, Pcm
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 53945
Homologene: 40959
Garin1a
Name: golgi associated RAB2 interactor 1A
Synonyms: Fam71f2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 245884
Homologene: 52974
Krt80
Name: keratin 80
Synonyms: 2310041I20Rik, 1200016G03Rik, Kb20
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 74127
VEGA: 15
Homologene: 66610
Zfy2
Name: zinc finger protein 2, Y-linked
Synonyms: Zfy-2
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 22768
Homologene: 56456
Spata9
Name: spermatogenesis associated 9
Synonyms: 4930599C08Rik, 1700030K01Rik, A930023H06Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75571
VEGA: 13
Homologene: 12609
Slc7a11
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
Synonyms: System xc, xc, xCT, sut, 9930009M05Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26570
Homologene: 22684
Or1o4
Name: olfactory receptor family 1 subfamily O member 4
Synonyms: MOR156-1, GA_x6K02T2PSCP-1720261-1719344, Olfr99
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258508
Homologene: 74030
Pbxip1
Name: pre B cell leukemia transcription factor interacting protein 1
Synonyms: 4732463H20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229534
Homologene: 10740
Thg1l
Name: tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
Synonyms: 1700121M19Rik, 5730409G07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66628
Homologene: 5959
Or10ag57
Name: olfactory receptor family 10 subfamily AG member 57
Synonyms: GA_x6K02T2Q125-48880078-48881058, MOR264-1, Olfr1122
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259033
Homologene: 73977
Tspan32
Name: tetraspanin 32
Synonyms: D7Wsu37e, Tssc6, Art-1, Tspan32, Phemx
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27027
Homologene: 10650
Rasgrf2
Name: RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms: Grf2, 6330417G04Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19418
HGNC: HGNC:9876
Homologene: 2169
Pramel12
Name: PRAME like 12
Synonyms: 4732496O08Rik, Pramef8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242736
Homologene: 128393
Harbi1
Name: harbinger transposase derived 1
Synonyms: D230010M03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241547
Homologene: 24535
Or8k17
Name: olfactory receptor family 8 subfamily K member 17
Synonyms: GA_x6K02T2Q125-47716657-47715716, MOR187-2, Olfr1048
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259016
Marchf8
Name: membrane associated ring-CH-type finger 8
Synonyms: 1300017E09Rik, Mir, March8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71779
Homologene: 12412
Rfxank
Name: regulatory factor X-associated ankyrin-containing protein
Synonyms: Tvl1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19727
HGNC: HGNC:9987
Homologene: 2760
Lbx1
Name: ladybird homeobox 1
Synonyms: Lbx1h
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16814
Homologene: 4784
Vmn1r21
Name: vomeronasal 1 receptor 21
Synonyms: V1rc28
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171201
Homologene: 74363
Mfsd7c
Name:
Type: Gene
Species: Mouse
Chromosome: 12
5730480H06Rik
Name: RIKEN cDNA 5730480H06 gene
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70592
Gm6337
Name: predicted gene 6337
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 622640
Homologene: 115686
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 45,818,182 bp
  • A to C, chromosome 1 at 45,910,969 bp
  • A to T, chromosome 1 at 118,305,254 bp
  • A to C, chromosome 1 at 157,157,381 bp
  • C to T, chromosome 1 at 172,368,972 bp
  • A to T, chromosome 1 at 175,900,914 bp
  • A to G, chromosome 2 at 12,980,555 bp
  • A to G, chromosome 2 at 36,688,673 bp
  • C to T, chromosome 2 at 86,236,518 bp
  • C to T, chromosome 2 at 87,388,578 bp
  • T to C, chromosome 2 at 91,712,892 bp
  • C to T, chromosome 2 at 104,762,923 bp
  • T to A, chromosome 3 at 32,437,847 bp
  • A to T, chromosome 3 at 50,424,052 bp
  • A to G, chromosome 3 at 83,838,239 bp
  • A to T, chromosome 3 at 88,762,120 bp
  • A to G, chromosome 3 at 89,445,595 bp
  • A to G, chromosome 3 at 105,959,186 bp
  • G to A, chromosome 3 at 135,857,564 bp
  • G to A, chromosome 4 at 43,372,728 bp
  • T to A, chromosome 4 at 112,839,274 bp
  • A to T, chromosome 4 at 143,419,136 bp
  • T to C, chromosome 5 at 14,675,663 bp
  • A to C, chromosome 5 at 25,283,106 bp
  • T to A, chromosome 5 at 34,669,196 bp
  • T to A, chromosome 5 at 48,379,427 bp
  • G to A, chromosome 5 at 115,581,529 bp
  • T to C, chromosome 6 at 29,286,073 bp
  • T to A, chromosome 6 at 38,014,806 bp
  • T to A, chromosome 6 at 38,489,279 bp
  • G to T, chromosome 6 at 57,843,925 bp
  • A to T, chromosome 6 at 116,338,098 bp
  • T to C, chromosome 7 at 4,542,823 bp
  • A to T, chromosome 7 at 111,074,432 bp
  • A to G, chromosome 7 at 141,439,653 bp
  • A to T, chromosome 7 at 143,011,095 bp
  • C to A, chromosome 8 at 70,131,435 bp
  • A to G, chromosome 8 at 108,950,717 bp
  • T to A, chromosome 9 at 18,637,019 bp
  • T to C, chromosome 9 at 22,129,606 bp
  • C to T, chromosome 9 at 63,482,633 bp
  • T to C, chromosome 9 at 104,099,211 bp
  • C to A, chromosome 9 at 108,719,249 bp
  • G to A, chromosome 10 at 4,746,808 bp
  • G to A, chromosome 10 at 8,751,236 bp
  • A to C, chromosome 10 at 39,147,379 bp
  • T to A, chromosome 10 at 81,306,416 bp
  • A to G, chromosome 10 at 106,863,375 bp
  • A to G, chromosome 10 at 127,276,529 bp
  • T to C, chromosome 11 at 22,096,096 bp
  • A to G, chromosome 11 at 43,490,948 bp
  • T to C, chromosome 11 at 45,955,450 bp
  • A to T, chromosome 11 at 53,398,257 bp
  • T to A, chromosome 11 at 53,986,581 bp
  • T to G, chromosome 11 at 106,017,374 bp
  • GTAGTGTATA to GTA, chromosome 12 at 85,803,148 bp
  • A to G, chromosome 12 at 108,793,693 bp
  • T to C, chromosome 13 at 41,118,068 bp
  • G to T, chromosome 13 at 48,804,628 bp
  • T to C, chromosome 13 at 75,977,773 bp
  • G to A, chromosome 13 at 91,982,677 bp
  • C to T, chromosome 14 at 6,050,613 bp
  • T to A, chromosome 14 at 88,468,611 bp
  • T to A, chromosome 15 at 73,512,773 bp
  • C to T, chromosome 15 at 76,003,037 bp
  • G to A, chromosome 15 at 84,955,467 bp
  • T to G, chromosome 15 at 101,370,003 bp
  • C to G, chromosome 16 at 72,989,790 bp
  • A to G, chromosome 17 at 20,463,203 bp
  • A to G, chromosome 17 at 37,279,820 bp
  • T to C, chromosome 18 at 32,093,635 bp
  • C to A, chromosome 19 at 4,041,668 bp
  • A to G, chromosome 19 at 4,787,416 bp
  • A to G, chromosome 19 at 45,234,920 bp
  • T to C, chromosome Y at 2,133,421 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8218 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067637-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.