Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8220Btlr/Mmmh
Stock Number:
067638-MU
Citation ID:
RRID:MMRRC_067638-MU
Other Names:
R8220 (G1)
Major Collection:

Strain Information

Ednrb
Name: endothelin receptor type B
Synonyms: ETb, Sox10m1, ETR-b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13618
VEGA: 14
HGNC: HGNC:3180
Homologene: 89
Rest
Name: RE1-silencing transcription factor
Synonyms: 2610008J04Rik, NRSF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19712
HGNC: HGNC:9966
Homologene: 4099
Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Becn1
Name: beclin 1, autophagy related
Synonyms: Atg6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56208
HGNC: HGNC:1034
Homologene: 2794
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 31,196,800 bp
  • C to T, chromosome 1 at 37,875,625 bp
  • T to A, chromosome 1 at 75,133,867 bp
  • C to T, chromosome 1 at 80,528,649 bp
  • T to C, chromosome 1 at 111,861,707 bp
  • G to T, chromosome 1 at 150,432,413 bp
  • T to A, chromosome 1 at 188,728,666 bp
  • T to A, chromosome 1 at 193,334,248 bp
  • T to C, chromosome 2 at 37,219,779 bp
  • T to C, chromosome 2 at 65,690,276 bp
  • C to T, chromosome 2 at 86,228,965 bp
  • C to T, chromosome 2 at 87,990,152 bp
  • T to C, chromosome 2 at 90,266,043 bp
  • A to T, chromosome 2 at 144,548,066 bp
  • A to T, chromosome 3 at 53,656,507 bp
  • A to G, chromosome 3 at 89,661,241 bp
  • C to A, chromosome 3 at 138,123,848 bp
  • A to T, chromosome 4 at 4,076,872 bp
  • T to A, chromosome 4 at 24,536,375 bp
  • T to G, chromosome 4 at 43,568,510 bp
  • T to C, chromosome 5 at 24,406,785 bp
  • G to A, chromosome 5 at 77,282,478 bp
  • T to A, chromosome 5 at 87,001,443 bp
  • T to A, chromosome 5 at 143,701,589 bp
  • A to T, chromosome 6 at 24,876,880 bp
  • C to A, chromosome 6 at 70,726,682 bp
  • T to A, chromosome 6 at 95,588,946 bp
  • T to C, chromosome 7 at 3,822,904 bp
  • A to T, chromosome 7 at 4,993,851 bp
  • T to A, chromosome 7 at 13,131,500 bp
  • A to G, chromosome 7 at 43,694,074 bp
  • T to C, chromosome 7 at 83,947,160 bp
  • T to A, chromosome 7 at 104,276,826 bp
  • A to G, chromosome 7 at 128,130,918 bp
  • G to A, chromosome 7 at 135,698,121 bp
  • T to C, chromosome 7 at 140,006,140 bp
  • T to A, chromosome 8 at 3,158,702 bp
  • A to G, chromosome 8 at 45,039,956 bp
  • T to C, chromosome 8 at 86,844,687 bp
  • A to G, chromosome 8 at 90,258,080 bp
  • G to T, chromosome 9 at 19,497,021 bp
  • G to T, chromosome 9 at 20,349,052 bp
  • T to A, chromosome 9 at 50,575,316 bp
  • A to T, chromosome 9 at 57,577,670 bp
  • T to A, chromosome 9 at 60,610,330 bp
  • G to A, chromosome 9 at 92,295,660 bp
  • A to G, chromosome 9 at 98,903,528 bp
  • C to T, chromosome 10 at 7,676,333 bp
  • C to T, chromosome 10 at 29,147,268 bp
  • A to G, chromosome 10 at 33,450,985 bp
  • A to T, chromosome 10 at 77,787,951 bp
  • A to G, chromosome 10 at 81,145,004 bp
  • C to A, chromosome 10 at 127,662,776 bp
  • A to T, chromosome 10 at 128,246,396 bp
  • C to T, chromosome 11 at 9,434,299 bp
  • C to T, chromosome 11 at 17,945,690 bp
  • C to T, chromosome 11 at 32,214,563 bp
  • T to C, chromosome 11 at 58,990,394 bp
  • T to C, chromosome 11 at 76,672,877 bp
  • C to T, chromosome 11 at 96,771,059 bp
  • C to T, chromosome 11 at 101,296,279 bp
  • T to C, chromosome 12 at 113,156,755 bp
  • T to C, chromosome 13 at 23,621,939 bp
  • A to G, chromosome 13 at 73,685,770 bp
  • T to C, chromosome 14 at 15,358,004 bp
  • C to T, chromosome 14 at 34,045,919 bp
  • G to T, chromosome 14 at 43,232,717 bp
  • C to T, chromosome 14 at 53,461,645 bp
  • A to T, chromosome 14 at 103,821,705 bp
  • T to C, chromosome 15 at 12,592,163 bp
  • C to T, chromosome 15 at 76,178,297 bp
  • C to T, chromosome 15 at 77,764,547 bp
  • A to G, chromosome 16 at 31,887,415 bp
  • A to T, chromosome 16 at 32,755,327 bp
  • A to T, chromosome 16 at 88,773,835 bp
  • A to T, chromosome 17 at 12,692,071 bp
  • A to G, chromosome 17 at 23,900,692 bp
  • A to G, chromosome 17 at 24,849,890 bp
  • A to G, chromosome 17 at 42,502,663 bp
  • A to G, chromosome 18 at 37,006,571 bp
  • A to T, chromosome 19 at 41,215,087 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8220 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067638-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.