Strain Name:
C57BL/6J-MtgxR8222Btlr/Mmmh
Stock Number:
067640-MU
Citation ID:
RRID:MMRRC_067640-MU
Other Names:
R8222 (G1)
Major Collection:

Strain Information

Slc12a6
Name: solute carrier family 12, member 6
Synonyms: KCC3, gaxp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 107723
Homologene: 21069
Sort1
Name: sortilin 1
Synonyms: Ntr3, 2900053A11Rik, sortilin, Ntsr3, neurotensin receptor 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20661
Homologene: 136097
Tfdp2
Name: transcription factor Dp 2
Synonyms: A330080J22Rik, DP-3, DP3, 1110029I05Rik, DP3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 211586
Homologene: 4578
Utp20
Name: UTP20 small subunit processome component
Synonyms: 3830408P06Rik, DRIM, mDRIM
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 9630045K08Rik, 4733401D09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Cyp11b2
Name: cytochrome P450, family 11, subfamily b, polypeptide 2
Synonyms: Cyp11b, Cyp11b-2, aldosterone synthase, steroid-11-beta-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13072
Homologene: 128035
Polk
Name: polymerase (DNA directed), kappa
Synonyms: Dinb1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27015
VEGA: 13
HGNC: HGNC:9183
Homologene: 32140
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: 9530048I21Rik, 3010027A04Rik, 2410104C19Rik, Yod
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 77087
Homologene: 69134
Ipo5
Name: importin 5
Synonyms: IMB3, 1110011C18Rik, 5730478E03Rik, importin beta 3, RanBP5, Kpnb3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 70572
VEGA: 14
HGNC: HGNC:6402
Homologene: 1710
Mdn1
Name: midasin AAA ATPase 1
Synonyms: D4Abb1e, 4833432B22Rik, LOC213784
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100019
Homologene: 39689
Brpf1
Name: bromodomain and PHD finger containing, 1
Synonyms: 4833438B11Rik, 4930540D11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 78783
Homologene: 31251
Atg2b
Name: autophagy related 2B
Synonyms: C630028L02Rik, 2410024A21Rik, C030004M05Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 76559
VEGA: 12
Homologene: 9974
Irak4
Name: interleukin-1 receptor-associated kinase 4
Synonyms: NY-REN-64, IRAK-4, 8430405M07Rik, 9330209D03Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 266632
Homologene: 41109
Tefm
Name: transcription elongation factor, mitochondrial
Synonyms: 1110002N22Rik, FLJ22729
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68550
Homologene: 11657
Cdk1
Name: cyclin dependent kinase 1
Synonyms: p34CDC2, Cdc2a, Cdc2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12534
HGNC: HGNC:1722
Homologene: 68203
Nlrp3
Name: NLR family, pyrin domain containing 3
Synonyms: Cias1, Mmig1, NALP3, cryopyrin, Pypaf1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216799
Homologene: 3600
R3hcc1l
Name: R3H domain and coiled-coil containing 1 like
Synonyms: 1700036B12Rik, D19Ertd386e
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 52013
Homologene: 8707
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: A230048G03Rik, 2010013B10Rik, D330050P16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Reln
Name: reelin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Tmprss7
Name: transmembrane serine protease 7
Synonyms: LOC385645, matriptase-3, B230219I23Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208171
Homologene: 18178
Pkdrej
Name: polycystin (PKD) family receptor for egg jelly
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18766
VEGA: 15
HGNC: HGNC:9015
Homologene: 4427
Gas6
Name: growth arrest specific 6
Synonyms: Gas-6, growth arrest-specific, GAS 6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14456
HGNC: HGNC:4168
Homologene: 638
B4galt3
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3
Synonyms: beta4GalT-III, 9530061M23Rik, R74981, ESTM26
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 57370
HGNC: HGNC:926
Homologene: 48241
Lrp4
Name: low density lipoprotein receptor-related protein 4
Synonyms: 6430526J12Rik, Megf7, mdig
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228357
HGNC: HGNC:6696
Homologene: 17964
Esyt1
Name: extended synaptotagmin-like protein 1
Synonyms: Fam62a, vp115, Mbc2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 23943
VEGA: 10
Homologene: 40794
Bace1
Name: beta-site APP cleaving enzyme 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23821
HGNC: HGNC:933
Homologene: 8014
Vmn2r56
Name: vomeronasal 2, receptor 56
Synonyms: EG629079
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 629079
Homologene: 104040
Trpm8
Name: transient receptor potential cation channel, subfamily M, member 8
Synonyms: CMR1, TRPP8, Trp-p8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 171382
Homologene: 23433
Cfap251
Name: cilia and flagella associated protein 251
Synonyms: 4930415N18Rik, 4933428F06Rik, Wdr66
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269701
Homologene: 16964
Sel1l3
Name: sel-1 suppressor of lin-12-like 3 (C. elegans)
Synonyms: 2310045A20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231238
Homologene: 9054
Ceacam20
Name: CEA cell adhesion molecule 20
Synonyms: 9130012D09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71601
Homologene: 19010
Dlk2
Name: delta like non-canonical Notch ligand 2
Synonyms: Egfl9
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106565
Homologene: 11397
Mlxip
Name: MLX interacting protein
Synonyms: Mondoa, Mir, bHLHe36
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208104
Homologene: 22851
Serpina3m
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3M
Synonyms: Spi-2rs1, contrapsin-like, 3e46, MMCM7, alpha-1 antiproteinase, Spi2-rs1, Spi2.4, MMSPi2.4, antitrypsin, Spi-2l
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20717
HGNC: HGNC:16
Homologene: 111129
Prdm9
Name: PR domain containing 9
Synonyms: repro7, Dsbc1, Rcr1, G1-419-29, Meisetz
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 213389
Homologene: 104139
Rassf8
Name: Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
Synonyms: mHoj-1, 5133400D11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71323
Homologene: 5219
Or51f5
Name: olfactory receptor family 51 subfamily F member 5
Synonyms: GA_x6K02T2PBJ9-5491151-5492095, MOR14-2, Olfr561
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259096
Homologene: 133029
Aldh18a1
Name: aldehyde dehydrogenase 18 family, member A1
Synonyms: Pycs, 2810433K04Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56454
HGNC: HGNC:9722
Homologene: 2142
Pramel26
Name: PRAME like 26
Synonyms: Gm13084
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381569
Homologene: 77858
Stx6
Name: syntaxin 6
Synonyms: 2310039E05Rik, 2410005I16Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 58244
Homologene: 115622
Or5l13
Name: olfactory receptor family 5 subfamily L member 13
Synonyms: Olfr1156, MOR174-3, GA_x6K02T2Q125-49433499-49432537
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258814
HGNC: HGNC:8351
Homologene: 72031
Grik2
Name: glutamate receptor, ionotropic, kainate 2 (beta 2)
Synonyms: Glurbeta2, Glur-6, C130030K03Rik, Glur6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14806
HGNC: HGNC:4580
Homologene: 40717
Cnnm4
Name: cyclin M4
Synonyms: Acdp4, 5430430O18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 94220
HGNC: HGNC:105
Homologene: 75662
Mau2
Name: MAU2 sister chromatid cohesion factor
Synonyms: 9130404D08Rik, A930019L04Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74549
Homologene: 12574
Nab2
Name: Ngfi-A binding protein 2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17937
HGNC: HGNC:7627
Homologene: 4353
Cma2
Name: chymase 2, mast cell
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545055
VEGA: 14
Homologene: 115745
Ganc
Name: glucosidase, alpha; neutral C
Synonyms: 5830445O15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76051
HGNC: HGNC:4139
Homologene: 25627
Mn1
Name: meningioma 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433938
HGNC: HGNC:7180
Homologene: 37620
Vmn1r159
Name: vomeronasal 1 receptor 159
Synonyms: Gm16507
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 670857
Homologene: 104166
Or13p10
Name: olfactory receptor family 13 subfamily P member 10
Synonyms: MOR258-5, Olfr62, GA_x6K02T2QD9B-18877756-18876809, IH12
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18363
Slc25a3
Name: solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3
Synonyms: Phc, 5730556H19Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18674
Homologene: 37649
Ube2q2l
Name: ubiquitin conjugating enzyme E2 Q2 like
Synonyms: E330021D16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 100502936
Or5d36
Name: olfactory receptor family 5 subfamily D member 36
Synonyms: GA_x6K02T2Q125-49563265-49562315, MOR174-8, Olfr1163
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258638
Homologene: 17328
Ugt2a2
Name: UDP glucuronosyltransferase 2 family, polypeptide A2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 552899
Homologene: 115736
Mup2
Name: major urinary protein 2
Synonyms: Mup-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17841
Homologene: 74304
BC106179
Name: cDNA sequence BC106179
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 320099
Smek1
Name:
Type: Gene
Species: Mouse
Chromosome: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,506,536 bp
  • A to G, chromosome 1 at 88,325,668 bp
  • A to G, chromosome 1 at 155,198,143 bp
  • G to A, chromosome 1 at 171,272,683 bp
  • T to C, chromosome 2 at 87,949,444 bp
  • T to A, chromosome 2 at 88,071,037 bp
  • T to A, chromosome 2 at 91,474,741 bp
  • T to A, chromosome 2 at 112,339,525 bp
  • T to C, chromosome 2 at 120,446,452 bp
  • T to A, chromosome 3 at 108,334,635 bp
  • A to G, chromosome 3 at 145,128,038 bp
  • T to C, chromosome 4 at 32,707,477 bp
  • A to C, chromosome 4 at 60,138,454 bp
  • T to C, chromosome 4 at 118,665,916 bp
  • A to T, chromosome 4 at 143,810,323 bp
  • G to A, chromosome 5 at 21,931,477 bp
  • A to G, chromosome 5 at 53,187,954 bp
  • A to G, chromosome 5 at 87,460,510 bp
  • A to T, chromosome 5 at 111,418,680 bp
  • T to A, chromosome 5 at 123,302,423 bp
  • T to G, chromosome 5 at 123,447,533 bp
  • C to T, chromosome 6 at 113,310,038 bp
  • A to G, chromosome 6 at 136,400,884 bp
  • G to A, chromosome 6 at 145,820,057 bp
  • T to A, chromosome 7 at 12,711,033 bp
  • T to C, chromosome 7 at 19,971,693 bp
  • G to T, chromosome 7 at 22,843,183 bp
  • G to T, chromosome 7 at 102,774,892 bp
  • T to C, chromosome 8 at 13,470,276 bp
  • A to T, chromosome 8 at 70,033,177 bp
  • A to T, chromosome 8 at 122,895,608 bp
  • T to C, chromosome 9 at 45,857,193 bp
  • T to C, chromosome 9 at 96,310,613 bp
  • A to G, chromosome 10 at 49,573,648 bp
  • A to G, chromosome 10 at 69,340,596 bp
  • A to G, chromosome 10 at 88,778,372 bp
  • A to G, chromosome 10 at 91,118,191 bp
  • C to A, chromosome 10 at 127,662,776 bp
  • C to T, chromosome 10 at 128,511,778 bp
  • A to G, chromosome 11 at 59,548,788 bp
  • A to G, chromosome 11 at 80,140,404 bp
  • A to G, chromosome 12 at 101,041,905 bp
  • A to T, chromosome 12 at 104,392,701 bp
  • A to G, chromosome 12 at 105,652,216 bp
  • T to C, chromosome 13 at 96,495,515 bp
  • T to C, chromosome 14 at 55,973,270 bp
  • A to G, chromosome 14 at 120,920,002 bp
  • T to C, chromosome 15 at 74,856,210 bp
  • C to T, chromosome 15 at 76,179,174 bp
  • T to A, chromosome 15 at 85,817,439 bp
  • T to A, chromosome 15 at 94,561,229 bp
  • A to T, chromosome 16 at 23,224,305 bp
  • A to T, chromosome 16 at 45,658,098 bp
  • A to G, chromosome 17 at 15,544,773 bp
  • A to G, chromosome 17 at 46,302,458 bp
  • A to G, chromosome 17 at 78,801,701 bp
  • C to T, chromosome 19 at 40,573,852 bp
  • T to A, chromosome 19 at 42,576,177 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8222 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067640-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.