Strain Name:
C57BL/6J-MtgxR8277Btlr/Mmmh
Stock Number:
067700-MU
Citation ID:
RRID:MMRRC_067700-MU
Other Names:
R8277 (G1)
Major Collection:

Strain Information

Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Rbpms2
Name: RNA binding protein with multiple splicing 2
Synonyms: 2400008B06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71973
VEGA: 9
Homologene: 49895
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: Rpo2-1, 220kDa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Plxna1
Name: plexin A1
Synonyms: NOV, Plxn1, PlexA1, 2600013D04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18844
HGNC: HGNC:9099
Homologene: 56426
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, MCPH5, D330028K02Rik, Aspm, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 212285
Homologene: 9064
Ddx52
Name: DExD box helicase 52
Synonyms: 2700029C06Rik, ROK1, DEAD (Asp-Glu-Ala-Asp) box polypeptide 52
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78394
Homologene: 5093
Atcay
Name: ataxia, cerebellar, Cayman type
Synonyms: 3322401A10Rik, BNIP-H, ji
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16467
HGNC: HGNC:779
Homologene: 13206
Apoe
Name: apolipoprotein E
Synonyms: Apoe
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11816
HGNC: HGNC:613
Homologene: 30951
Entr1
Name: endosome associated trafficking regulator 1
Synonyms: C630038K21Rik, Sdccag3, C330016H24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68112
Homologene: 74578
Rmdn3
Name: regulator of microtubule dynamics 3
Synonyms: 1200015F23Rik, Fam82a2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67809
Homologene: 34926
Septin2
Name: septin 2
Synonyms: Sept2, Nedd5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18000
HGNC: HGNC:7729
Homologene: 3243
Dync2i2
Name: dynein 2 intermediate chain 2
Synonyms: Wdr34, 3200002I06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71820
Homologene: 14156
Pask
Name: PAS domain containing serine/threonine kinase
Synonyms: Paskin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269224
Homologene: 9038
Tctn1
Name: tectonic family member 1
Synonyms: G730031O11Rik, Tect1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 654470
Homologene: 49770
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Cola2, Cola-2, Col1a-2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 665700
Homologene: 90772
Snapc4
Name: small nuclear RNA activating complex, polypeptide 4
Synonyms: 5730436L13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227644
Homologene: 2321
Myh8
Name: myosin, heavy polypeptide 8, skeletal muscle, perinatal
Synonyms: Myhsp, Myhs-p, 4832426G23Rik, MyHC-pn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17885
HGNC: HGNC:7578
Homologene: 68256
Gucy2d
Name: guanylate cyclase 2d
Synonyms: guanylyl cyclase D
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14918
Homologene: 71540
Per2
Name: period circadian clock 2
Synonyms: mPer2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18627
HGNC: HGNC:8846
Homologene: 7885
Vmn2r15
Name: vomeronasal 2, receptor 15
Synonyms: EG211223
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 211223
Homologene: 129606
Lingo4
Name: leucine rich repeat and Ig domain containing 4
Synonyms: LERN4, Lrrn6d, A530050P17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 320747
Homologene: 18563
Ablim1
Name: actin-binding LIM protein 1
Synonyms: abLIM-S, 9330196J19Rik, 2210411C18Rik, abLIM-L, abLIM-M, 4833406P10Rik, Limab1, 2610209L21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226251
HGNC: HGNC:78
Homologene: 40994
Chrm2
Name: cholinergic receptor, muscarinic 2, cardiac
Synonyms: Chrm-2, M2, AChR M2, muscarinic acetylcholine receptor 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243764
HGNC: HGNC:1951
Homologene: 20190
Pcnx2
Name: pecanex homolog 2
Synonyms: Pcnxl2, E330039K12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Mmrn1
Name: multimerin 1
Synonyms: Emilin4, 4921530G03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70945
HGNC: HGNC:7178
Homologene: 49134
Unc5c
Name: unc-5 netrin receptor C
Synonyms: B130051O18Rik, Unc5h3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22253
Homologene: 2765
Vmn2r11
Name: vomeronasal 2, receptor 11
Synonyms: EG384219
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 384219
Homologene: 129606
Nr2c2ap
Name: nuclear receptor 2C2-associated protein
Synonyms: 2310073E15Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 75692
Homologene: 18532
Cfh
Name: complement component factor h
Synonyms: Mud-1, Sas-1, Sas1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12628
HGNC: HGNC:4883
Homologene: 20086
Cd46
Name: CD46 antigen, complement regulatory protein
Synonyms: CD46, Mcp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17221
HGNC: HGNC:6953
Homologene: 7832
Serpinb3a
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3A
Synonyms: Sqn5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20248
Homologene: 76659
Zfp763
Name: zinc finger protein 763
Synonyms: 1700065O13Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73451
Homologene: 66274
Nab2
Name: Ngfi-A binding protein 2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17937
HGNC: HGNC:7627
Homologene: 4353
Mro
Name: maestro
Synonyms: 4933435E20Rik, 4930507C04Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 71263
Homologene: 41729
Hao2
Name: hydroxyacid oxidase 2
Synonyms: Hao3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56185
HGNC: HGNC:4810
Homologene: 97412
Mrps26
Name: mitochondrial ribosomal protein S26
Synonyms: GI008, Rpms13, MRP-S13, MRP-S26
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99045
Homologene: 12778
Tgoln1
Name: trans-golgi network protein
Synonyms: TGN38A, TGN38, Ttgn1, D6Ertd384e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22134
Homologene: 137224
Slc35f3
Name: solute carrier family 35, member F3
Synonyms: B230375D17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 210027
Homologene: 62481
Iqcf1
Name: IQ motif containing F1
Synonyms: 1700055J15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74267
Homologene: 45119
Or1p1b
Name: olfactory receptor family 1 subfamily P member 1B
Synonyms: GA_x6K02T2P1NL-4385196-4386167, Olfr404-ps1, MOR133-5P
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 257920
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 91,420,552 bp
  • C to T, chromosome 1 at 93,325,363 bp
  • T to A, chromosome 1 at 93,499,308 bp
  • C to A, chromosome 1 at 107,046,240 bp
  • T to C, chromosome 1 at 139,455,010 bp
  • A to G, chromosome 1 at 140,101,609 bp
  • A to T, chromosome 1 at 195,064,722 bp
  • A to G, chromosome 2 at 26,365,710 bp
  • G to A, chromosome 2 at 26,384,766 bp
  • A to G, chromosome 2 at 30,033,874 bp
  • A to C, chromosome 2 at 31,369,177 bp
  • T to C, chromosome 2 at 119,146,424 bp
  • T to A, chromosome 2 at 130,564,427 bp
  • A to G, chromosome 3 at 94,402,624 bp
  • A to G, chromosome 3 at 98,880,384 bp
  • G to A, chromosome 3 at 141,768,612 bp
  • A to G, chromosome 5 at 62,613,992 bp
  • A to T, chromosome 5 at 109,054,967 bp
  • A to T, chromosome 5 at 109,293,104 bp
  • C to T, chromosome 5 at 109,293,444 bp
  • A to G, chromosome 5 at 122,264,368 bp
  • G to A, chromosome 6 at 4,516,410 bp
  • G to A, chromosome 6 at 36,523,276 bp
  • A to G, chromosome 6 at 60,977,236 bp
  • G to A, chromosome 6 at 72,616,855 bp
  • G to A, chromosome 6 at 89,357,180 bp
  • C to T, chromosome 7 at 19,698,378 bp
  • T to A, chromosome 7 at 98,443,475 bp
  • C to T, chromosome 8 at 70,132,481 bp
  • A to T, chromosome 8 at 125,866,016 bp
  • T to A, chromosome 8 at 126,389,186 bp
  • T to A, chromosome 9 at 65,649,413 bp
  • T to C, chromosome 9 at 106,501,878 bp
  • C to A, chromosome 10 at 81,214,812 bp
  • G to A, chromosome 10 at 127,665,299 bp
  • T to C, chromosome 11 at 67,292,909 bp
  • G to A, chromosome 11 at 69,748,056 bp
  • C to T, chromosome 11 at 74,240,016 bp
  • T to C, chromosome 11 at 83,955,114 bp
  • T to C, chromosome 17 at 33,033,320 bp
  • T to A, chromosome 17 at 53,641,253 bp
  • A to T, chromosome 18 at 73,864,061 bp
  • C to A, chromosome 19 at 57,215,919 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8277 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067700-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.