Strain Name:
C57BL/6J-MtgxR8394Btlr/Mmmh
Stock Number:
067758-MU
Citation ID:
RRID:MMRRC_067758-MU
Other Names:
R8394 (G1)
Major Collection:

Strain Information

Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19213
Homologene: 10304
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Arl8b
Name: ADP-ribosylation factor-like 8B
Synonyms: 2610313E07Rik, Arl10c, 3100002J04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67166
Homologene: 10056
Abcc8
Name: ATP-binding cassette, sub-family C member 8
Synonyms: SUR1, D930031B21Rik, Sur
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Senp7
Name: SUMO1/sentrin specific peptidase 7
Synonyms: 2410152H17Rik, 2900036C23Rik, 2810413I22Rik, 6030449K19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66315
Homologene: 10778
Zfp462
Name: zinc finger protein 462
Synonyms: 9430078C22Rik, Zfpip, Gt4-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242466
Homologene: 41430
Scube1
Name: signal peptide, CUB domain, EGF-like 1
Synonyms: A630023E24Rik, 7330410C13Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 64706
Homologene: 11224
Tmtc3
Name: transmembrane and tetratricopeptide repeat containing 3
Synonyms: mSmile, B130008E12Rik, 9130014E20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237500
Homologene: 27616
Il7r
Name: interleukin 7 receptor
Synonyms: IL-7 receptor alpha chain, IL-7Ralpha, CD127
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16197
VEGA: 15
HGNC: HGNC:6024
Homologene: 1646
Chrdl2
Name: chordin-like 2
Synonyms: Chl2, 1810022C01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69121
Homologene: 128795
Nectin2
Name: nectin cell adhesion molecule 2
Synonyms: MPH, Cd112, Pvrl2, nectin-2, Pvs
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19294
HGNC: HGNC:9707
Homologene: 86092
Ralbp1
Name: ralA binding protein 1
Synonyms: RLIP76, Rip1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19765
VEGA: 17
HGNC: HGNC:9841
Homologene: 4940
Efl1
Name: elongation factor like GTPase 1
Synonyms: 4932434J20Rik, Eftud1, 6030468D11Rik, D7Ertd791e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101592
Homologene: 11599
Txndc12
Name: thioredoxin domain containing 12 (endoplasmic reticulum)
Synonyms: 0610040B21Rik, ERp16
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66073
Homologene: 41074
Baiap3
Name: BAI1-associated protein 3
Synonyms: LOC381076
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 545192
HGNC: HGNC:948
Homologene: 20844
Sdk2
Name: sidekick cell adhesion molecule 2
Synonyms: 4632412F08Rik, 5330435L01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237979
Homologene: 10406
Strc
Name: stereocilin
Synonyms: DFNB16
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 140476
Homologene: 15401
Plcb1
Name: phospholipase C, beta 1
Synonyms: 3110043I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18795
Homologene: 22876
Vmn2r2
Name: vomeronasal 2, receptor 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100125586
Homologene: 129753
Klra2
Name: killer cell lectin-like receptor, subfamily A, member 2
Synonyms: Ly49b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16633
Homologene: 133782
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 628870
Homologene: 46008
Or52z13
Name: olfactory receptor family 52 subfamily Z member 13
Synonyms: Olfr618, MOR31-9, GA_x6K02T2PBJ9-6320148-6321104
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259049
Homologene: 72966
Zfp217
Name: zinc finger protein 217
Synonyms: 4933431C08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228913
Homologene: 4757
Vmn2r27
Name: vomeronasal 2, receptor27
Synonyms: EG232367
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232367
Evx2
Name: even-skipped homeobox 2
Synonyms: Evx-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14029
HGNC: HGNC:3507
Homologene: 74879
Nfil3
Name: nuclear factor, interleukin 3, regulated
Synonyms: E4BP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18030
HGNC: HGNC:7787
Homologene: 3928
1700129C05Rik
Name: RIKEN cDNA 1700129C05 gene
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67932
Homologene: 130749
Aldh3b2
Name: aldehyde dehydrogenase 3 family, member B2
Synonyms: C130048D07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 621603
HGNC: HGNC:411
Homologene: 84430
Cpt1b
Name: carnitine palmitoyltransferase 1b, muscle
Synonyms: Cpt1, M-CPTI, M-CPT I
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12895
HGNC: HGNC:2329
Homologene: 22548
Sphkap
Name: SPHK1 interactor, AKAP domain containing
Synonyms: SKIP, A930009L15Rik, 4930544G21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 77629
Homologene: 18172
Cmbl
Name: carboxymethylenebutenolidase homolog
Synonyms: 2310016A09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69574
Homologene: 100714
4930505A04Rik
Name: RIKEN cDNA 4930505A04 gene
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75087
Homologene: 18988
Hspa14
Name: heat shock protein 14
Synonyms: NST-1, HSP70L1, Hsp70-4, 70kDa
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 50497
Homologene: 74307
Tomm40l
Name: translocase of outer mitochondrial membrane 40-like
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 641376
Homologene: 134180
Tbc1d30
Name: TBC1 domain family, member 30
Synonyms: 4930505D03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74694
VEGA: 10
Homologene: 18930
Nme6
Name: NME/NM23 nucleoside diphosphate kinase 6
Synonyms: non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase), nm23-M6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54369
Homologene: 4231
Lce3c
Name: late cornified envelope 3C
Synonyms: 2300007B01Rik, Sprrl1, Eig3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 94060
Homologene: 137425
Ighv5-8
Name: immunoglobulin heavy variable V5-8
Synonyms: Gm17309
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 777782
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 83,276,076 bp
  • A to T, chromosome 1 at 171,221,207 bp
  • C to T, chromosome 2 at 3,512,633 bp
  • T to A, chromosome 2 at 19,445,935 bp
  • A to G, chromosome 2 at 74,655,977 bp
  • G to T, chromosome 2 at 121,379,009 bp
  • T to C, chromosome 2 at 135,317,790 bp
  • G to A, chromosome 2 at 170,119,651 bp
  • T to C, chromosome 3 at 64,137,437 bp
  • T to C, chromosome 3 at 92,945,453 bp
  • A to G, chromosome 4 at 55,011,862 bp
  • T to A, chromosome 4 at 108,856,223 bp
  • A to G, chromosome 6 at 108,783,283 bp
  • T to C, chromosome 6 at 124,191,817 bp
  • A to T, chromosome 6 at 131,245,310 bp
  • C to T, chromosome 7 at 19,733,212 bp
  • A to G, chromosome 7 at 46,154,553 bp
  • A to G, chromosome 7 at 82,762,778 bp
  • T to C, chromosome 7 at 100,017,085 bp
  • A to T, chromosome 7 at 103,598,197 bp
  • T to A, chromosome 9 at 109,835,326 bp
  • T to C, chromosome 10 at 100,446,946 bp
  • A to G, chromosome 10 at 107,886,465 bp
  • T to A, chromosome 10 at 121,347,108 bp
  • T to A, chromosome 11 at 30,454,880 bp
  • A to G, chromosome 11 at 113,838,716 bp
  • C to T, chromosome 12 at 113,655,193 bp
  • A to G, chromosome 13 at 52,967,813 bp
  • T to C, chromosome 14 at 59,142,174 bp
  • A to G, chromosome 15 at 9,516,418 bp
  • A to G, chromosome 15 at 31,585,395 bp
  • T to C, chromosome 15 at 83,608,291 bp
  • A to G, chromosome 15 at 89,422,287 bp
  • G to T, chromosome 16 at 56,169,827 bp
  • T to C, chromosome 16 at 59,558,288 bp
  • T to C, chromosome 17 at 25,250,122 bp
  • C to T, chromosome 17 at 65,852,753 bp
  • A to C, chromosome 19 at 3,979,461 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8394 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067758-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.