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Strain Name:
C57BL/6J-MtgxR8399Btlr/Mmmh
Stock Number:
067762-MU
Citation ID:
RRID:MMRRC_067762-MU
Other Names:
R8399 (G1)
Major Collection:

Strain Information

Chrnb4
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108015
VEGA: 9
HGNC: HGNC:1964
Homologene: 20196
Htr1d
Name: 5-hydroxytryptamine (serotonin) receptor 1D
Synonyms: Gpcr14, Htr1db
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15552
HGNC: HGNC:5289
Homologene: 20240
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Dnm1l
Name: dynamin 1-like
Synonyms: 6330417M19Rik, Drp1, python, Dnmlp1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74006
VEGA: 16
HGNC: HGNC:2973
Homologene: 6384
Plk4
Name: polo like kinase 4
Synonyms: Sak, Stk18
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20873
Homologene: 7962
Trappc9
Name: trafficking protein particle complex 9
Synonyms: 4632408O18Rik, 2900005P22Rik, Nibp, TRS130, 1810044A24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76510
VEGA: 15
Homologene: 81931
Smg1
Name: SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Synonyms: C130002K18Rik, 5430435M13Rik, 2610207I05Rik, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233789
Homologene: 56697
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 53,267,932 bp
  • C to T, chromosome 1 at 60,489,318 bp
  • G to A, chromosome 1 at 72,267,465 bp
  • T to C, chromosome 1 at 75,476,220 bp
  • G to A, chromosome 1 at 173,730,278 bp
  • T to C, chromosome 2 at 4,572,433 bp
  • T to C, chromosome 2 at 68,861,771 bp
  • A to C, chromosome 2 at 87,810,224 bp
  • T to C, chromosome 2 at 89,406,684 bp
  • T to C, chromosome 2 at 104,685,375 bp
  • A to C, chromosome 2 at 153,138,317 bp
  • T to A, chromosome 3 at 28,494,010 bp
  • C to T, chromosome 3 at 40,808,830 bp
  • A to T, chromosome 4 at 45,305,870 bp
  • G to A, chromosome 4 at 136,443,375 bp
  • T to C, chromosome 5 at 115,345,899 bp
  • A to T, chromosome 5 at 138,607,820 bp
  • A to T, chromosome 6 at 90,072,707 bp
  • C to T, chromosome 6 at 124,535,293 bp
  • T to C, chromosome 6 at 129,874,937 bp
  • C to T, chromosome 7 at 4,523,937 bp
  • A to C, chromosome 7 at 7,816,898 bp
  • T to C, chromosome 7 at 30,258,061 bp
  • T to C, chromosome 7 at 97,457,604 bp
  • T to C, chromosome 7 at 118,190,571 bp
  • C to G, chromosome 7 at 131,082,587 bp
  • C to T, chromosome 7 at 139,913,518 bp
  • C to T, chromosome 8 at 109,623,888 bp
  • T to A, chromosome 8 at 115,706,512 bp
  • T to C, chromosome 8 at 117,596,362 bp
  • T to A, chromosome 8 at 123,455,499 bp
  • T to A, chromosome 9 at 13,792,067 bp
  • T to A, chromosome 9 at 50,541,118 bp
  • A to C, chromosome 9 at 55,043,823 bp
  • C to T, chromosome 9 at 63,145,158 bp
  • T to C, chromosome 10 at 14,132,434 bp
  • T to A, chromosome 10 at 41,539,521 bp
  • C to A, chromosome 10 at 81,268,998 bp
  • G to T, chromosome 11 at 103,138,503 bp
  • T to A, chromosome 12 at 72,574,211 bp
  • G to A, chromosome 12 at 108,538,131 bp
  • G to A, chromosome 12 at 113,896,912 bp
  • A to G, chromosome 12 at 114,612,427 bp
  • G to T, chromosome 13 at 41,318,474 bp
  • A to G, chromosome 13 at 49,589,869 bp
  • A to T, chromosome 13 at 81,489,170 bp
  • G to A, chromosome 15 at 73,052,282 bp
  • T to A, chromosome 16 at 16,321,672 bp
  • G to T, chromosome 16 at 23,520,414 bp
  • T to C, chromosome 16 at 23,972,948 bp
  • T to A, chromosome 17 at 20,419,749 bp
  • T to C, chromosome 18 at 67,225,875 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8399 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067762-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.