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Strain Name:
C57BL/6J-MtgxR8429Btlr/Mmmh
Stock Number:
067774-MU
Citation ID:
RRID:MMRRC_067774-MU
Other Names:
R8429 (G1)
Major Collection:

Strain Information

Plekhj1
Name: pleckstrin homology domain containing, family J member 1
Synonyms: 9530063M10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78670
VEGA: 10
Homologene: 11389
Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20230
Homologene: 2232
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Pex7
Name: peroxisomal biogenesis factor 7
Synonyms: peroxisome biogenesis factor 7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18634
HGNC: HGNC:8860
Homologene: 242
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Ubtd1
Name: ubiquitin domain containing 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226122
VEGA: 19
Homologene: 11799
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 77,390,036 bp
  • C to T, chromosome 1 at 189,657,307 bp
  • G to T, chromosome 2 at 87,607,524 bp
  • G to A, chromosome 2 at 111,483,495 bp
  • G to T, chromosome 2 at 148,407,537 bp
  • C to T, chromosome 2 at 158,426,297 bp
  • A to T, chromosome 3 at 14,497,282 bp
  • G to A, chromosome 3 at 29,657,268 bp
  • A to G, chromosome 3 at 40,977,227 bp
  • C to A, chromosome 3 at 88,058,152 bp
  • T to C, chromosome 5 at 30,144,257 bp
  • T to C, chromosome 5 at 113,183,436 bp
  • C to T, chromosome 5 at 123,865,475 bp
  • A to G, chromosome 5 at 135,217,331 bp
  • T to C, chromosome 6 at 135,733,916 bp
  • C to A, chromosome 6 at 145,165,223 bp
  • T to C, chromosome 7 at 6,378,493 bp
  • T to A, chromosome 7 at 16,919,667 bp
  • T to C, chromosome 7 at 45,136,116 bp
  • A to T, chromosome 7 at 48,198,425 bp
  • T to C, chromosome 7 at 79,438,385 bp
  • T to A, chromosome 7 at 112,345,253 bp
  • T to A, chromosome 7 at 118,434,341 bp
  • G to A, chromosome 7 at 130,795,337 bp
  • C to T, chromosome 9 at 53,570,609 bp
  • T to C, chromosome 9 at 57,131,161 bp
  • T to C, chromosome 10 at 19,894,328 bp
  • C to T, chromosome 10 at 80,796,470 bp
  • G to T, chromosome 10 at 82,289,467 bp
  • A to G, chromosome 10 at 130,180,223 bp
  • T to A, chromosome 11 at 22,996,030 bp
  • A to T, chromosome 11 at 54,475,696 bp
  • A to T, chromosome 11 at 100,121,125 bp
  • A to T, chromosome 11 at 102,674,206 bp
  • A to T, chromosome 11 at 110,202,382 bp
  • G to T, chromosome 12 at 4,866,487 bp
  • A to T, chromosome 13 at 31,807,776 bp
  • A to G, chromosome 13 at 67,258,088 bp
  • A to G, chromosome 13 at 105,118,788 bp
  • G to A, chromosome 14 at 18,215,409 bp
  • A to G, chromosome 14 at 70,137,149 bp
  • C to T, chromosome 15 at 8,112,420 bp
  • A to G, chromosome 16 at 8,412,167 bp
  • A to G, chromosome 16 at 59,361,627 bp
  • A to T, chromosome 16 at 81,589,635 bp
  • G to T, chromosome 17 at 42,667,449 bp
  • A to T, chromosome 17 at 48,037,066 bp
  • A to G, chromosome 17 at 51,767,950 bp
  • T to C, chromosome 17 at 56,018,821 bp
  • A to G, chromosome 17 at 57,222,811 bp
  • T to A, chromosome 18 at 61,232,844 bp
  • A to G, chromosome 18 at 64,501,320 bp
  • C to T, chromosome 19 at 5,665,384 bp
  • G to T, chromosome 19 at 42,032,117 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8429 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067774-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.