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Strain Name:
C57BL/6J-MtgxR8299Btlr/Mmmh
Stock Number:
067787-MU
Citation ID:
RRID:MMRRC_067787-MU
Other Names:
R8299 (G1)
Major Collection:

Strain Information

Ednrb
Name: endothelin receptor type B
Synonyms: ETb, Sox10m1, ETR-b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13618
VEGA: 14
HGNC: HGNC:3180
Homologene: 89
Cep70
Name: centrosomal protein 70
Synonyms: 6720484E09Rik, C030018L16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68121
Homologene: 11387
Morc3
Name: microrchidia 3
Synonyms: 1110051N18Rik, D16Jhu32e, 1110051N18Rik, Zcwcc3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 338467
Homologene: 32257
Aspscr1
Name: ASPSCR1 tether for SLC2A4, UBX domain containing
Synonyms: ASPCR1, RCC17, ASPC, 1190006K01Rik, TUG, ASPL
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68938
Homologene: 41550
Etl4
Name: enhancer trap locus 4
Synonyms: Etl-4, E330027G05Rik, 6620402G01Rik, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 208618
Homologene: 10477
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: Ki-67, D630048A14Rik, Ki67
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Epha8
Name: Eph receptor A8
Synonyms: EphA8, Hek3, Eek
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13842
HGNC: HGNC:3391
Homologene: 22436
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 6,802,992 bp
  • A to C, chromosome 1 at 75,387,836 bp
  • A to G, chromosome 1 at 110,919,548 bp
  • G to A, chromosome 1 at 134,488,940 bp
  • T to A, chromosome 2 at 20,744,063 bp
  • T to C, chromosome 2 at 135,335,476 bp
  • T to G, chromosome 2 at 157,020,731 bp
  • A to G, chromosome 3 at 20,082,822 bp
  • T to C, chromosome 3 at 97,205,536 bp
  • C to T, chromosome 4 at 59,315,773 bp
  • T to C, chromosome 4 at 120,889,372 bp
  • G to T, chromosome 4 at 136,938,586 bp
  • T to C, chromosome 4 at 143,950,757 bp
  • T to A, chromosome 5 at 67,326,905 bp
  • A to G, chromosome 6 at 67,037,199 bp
  • A to G, chromosome 6 at 124,437,373 bp
  • T to A, chromosome 7 at 44,627,048 bp
  • G to A, chromosome 7 at 64,411,873 bp
  • T to C, chromosome 7 at 102,484,202 bp
  • T to C, chromosome 7 at 102,955,532 bp
  • A to T, chromosome 7 at 135,704,620 bp
  • T to C, chromosome 8 at 105,728,162 bp
  • G to A, chromosome 8 at 112,773,692 bp
  • A to T, chromosome 9 at 99,262,861 bp
  • T to C, chromosome 9 at 107,534,546 bp
  • C to T, chromosome 10 at 39,821,541 bp
  • C to A, chromosome 10 at 69,976,151 bp
  • G to T, chromosome 10 at 80,128,033 bp
  • A to G, chromosome 10 at 81,499,433 bp
  • T to A, chromosome 10 at 94,827,179 bp
  • T to C, chromosome 10 at 129,625,960 bp
  • T to C, chromosome 11 at 4,179,420 bp
  • C to T, chromosome 11 at 94,239,064 bp
  • T to C, chromosome 11 at 120,709,074 bp
  • A to G, chromosome 12 at 51,648,107 bp
  • A to G, chromosome 12 at 112,604,112 bp
  • T to C, chromosome 13 at 41,191,920 bp
  • A to G, chromosome 14 at 16,386,123 bp
  • T to C, chromosome 14 at 50,868,045 bp
  • G to A, chromosome 14 at 99,068,134 bp
  • A to G, chromosome 14 at 103,823,500 bp
  • T to C, chromosome 15 at 44,581,934 bp
  • T to A, chromosome 15 at 78,346,469 bp
  • A to T, chromosome 15 at 91,673,240 bp
  • A to C, chromosome 16 at 30,333,801 bp
  • A to G, chromosome 16 at 32,755,897 bp
  • T to C, chromosome 16 at 93,853,200 bp
  • T to G, chromosome 17 at 11,237,521 bp
  • G to T, chromosome 17 at 37,752,000 bp
  • T to C, chromosome 18 at 36,682,927 bp
  • T to C, chromosome 18 at 37,485,355 bp
  • T to A, chromosome 18 at 60,545,800 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8299 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067787-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.