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Strain Name:
C57BL/6J-MtgxR8309Btlr/Mmmh
Stock Number:
067794-MU
Citation ID:
RRID:MMRRC_067794-MU
Other Names:
R8309 (G1)
Major Collection:

Strain Information

Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235504
Homologene: 56571
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Myo1e
Name: myosin IE
Synonyms: 2310020N23Rik, 9130023P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71602
VEGA: 9
HGNC: HGNC:7599
Homologene: 55864
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Lars1
Name: leucyl-tRNA synthetase 1
Synonyms: 3110009L02Rik, 2310045K21Rik, Lars
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 4,347,089 bp
  • T to A, chromosome 1 at 34,117,511 bp
  • T to C, chromosome 1 at 46,139,872 bp
  • T to C, chromosome 1 at 52,151,245 bp
  • T to A, chromosome 1 at 63,306,591 bp
  • T to A, chromosome 1 at 72,319,127 bp
  • A to T, chromosome 1 at 75,172,944 bp
  • A to T, chromosome 1 at 93,312,851 bp
  • G to T, chromosome 1 at 127,909,918 bp
  • A to T, chromosome 1 at 131,263,328 bp
  • A to G, chromosome 2 at 30,032,189 bp
  • T to A, chromosome 2 at 35,389,587 bp
  • C to T, chromosome 2 at 59,874,234 bp
  • T to A, chromosome 2 at 119,960,930 bp
  • T to A, chromosome 2 at 146,404,866 bp
  • T to C, chromosome 2 at 160,753,933 bp
  • G to T, chromosome 3 at 58,417,123 bp
  • G to T, chromosome 3 at 79,083,202 bp
  • G to A, chromosome 3 at 87,810,442 bp
  • C to A, chromosome 3 at 108,080,116 bp
  • G to A, chromosome 3 at 109,027,342 bp
  • G to A, chromosome 3 at 144,437,604 bp
  • G to GC, chromosome 4 at 118,375,482 bp
  • A to G, chromosome 4 at 135,529,592 bp
  • A to G, chromosome 4 at 147,332,308 bp
  • A to T, chromosome 4 at 147,890,236 bp
  • T to A, chromosome 5 at 66,362,836 bp
  • A to T, chromosome 5 at 76,254,422 bp
  • A to G, chromosome 5 at 100,804,398 bp
  • G to A, chromosome 5 at 116,591,567 bp
  • T to A, chromosome 6 at 14,719,701 bp
  • A to G, chromosome 6 at 40,745,177 bp
  • A to G, chromosome 6 at 57,258,650 bp
  • A to G, chromosome 7 at 19,148,473 bp
  • G to A, chromosome 7 at 79,348,347 bp
  • T to A, chromosome 7 at 89,877,578 bp
  • T to C, chromosome 7 at 99,348,944 bp
  • A to G, chromosome 7 at 103,639,451 bp
  • A to C, chromosome 7 at 106,824,413 bp
  • A to G, chromosome 7 at 109,901,125 bp
  • C to T, chromosome 7 at 127,393,324 bp
  • T to C, chromosome 7 at 127,549,357 bp
  • A to G, chromosome 7 at 143,883,943 bp
  • A to T, chromosome 8 at 3,926,559 bp
  • A to G, chromosome 8 at 39,164,841 bp
  • A to G, chromosome 8 at 69,663,274 bp
  • A to T, chromosome 8 at 106,916,221 bp
  • A to G, chromosome 8 at 110,607,902 bp
  • A to T, chromosome 8 at 111,082,057 bp
  • A to C, chromosome 9 at 18,456,917 bp
  • C to T, chromosome 9 at 39,426,670 bp
  • A to G, chromosome 9 at 63,339,079 bp
  • A to T, chromosome 9 at 70,346,763 bp
  • A to T, chromosome 9 at 78,571,029 bp
  • G to A, chromosome 9 at 79,605,183 bp
  • C to T, chromosome 9 at 106,075,215 bp
  • G to A, chromosome 10 at 40,637,379 bp
  • T to C, chromosome 11 at 29,153,277 bp
  • C to T, chromosome 11 at 73,279,921 bp
  • T to C, chromosome 11 at 97,691,743 bp
  • T to C, chromosome 12 at 116,256,085 bp
  • G to A, chromosome 13 at 33,039,571 bp
  • G to T, chromosome 13 at 33,150,111 bp
  • T to G, chromosome 13 at 56,363,135 bp
  • T to C, chromosome 13 at 62,869,017 bp
  • G to A, chromosome 14 at 13,814,954 bp
  • A to T, chromosome 14 at 51,922,436 bp
  • G to A, chromosome 14 at 55,826,391 bp
  • C to T, chromosome 14 at 118,616,392 bp
  • C to G, chromosome 15 at 76,331,960 bp
  • T to G, chromosome 15 at 79,762,510 bp
  • A to G, chromosome 15 at 101,946,487 bp
  • A to G, chromosome 16 at 10,421,622 bp
  • A to T, chromosome 16 at 11,146,553 bp
  • A to G, chromosome 16 at 18,066,734 bp
  • A to G, chromosome 16 at 20,688,828 bp
  • G to A, chromosome 16 at 33,865,553 bp
  • A to G, chromosome 16 at 94,466,979 bp
  • A to T, chromosome 17 at 32,634,978 bp
  • T to C, chromosome 17 at 53,449,535 bp
  • T to C, chromosome 17 at 56,104,437 bp
  • T to A, chromosome 18 at 31,153,845 bp
  • T to C, chromosome 18 at 42,243,028 bp
  • A to G, chromosome 19 at 5,654,936 bp
  • A to G, chromosome 19 at 5,717,075 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8309 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067794-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.