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Strain Name:
C57BL/6J-MtgxR8311Btlr/Mmmh
Stock Number:
067796-MU
Citation ID:
RRID:MMRRC_067796-MU
Other Names:
R8311 (G1)
Major Collection:

Strain Information

Gga3
Name: golgi associated, gamma adaptin ear containing, ARF binding protein 3
Synonyms: C230037M19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 260302
Homologene: 121703
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: TrkA, Tkr
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Ccdc91
Name: coiled-coil domain containing 91
Synonyms: 1700086G08Rik, 1810060J02Rik, p56
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67015
Homologene: 10131
Bnc2
Name: basonuclin zinc finger protein 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242509
Homologene: 18243
Eif4ebp1
Name: eukaryotic translation initiation factor 4E binding protein 1
Synonyms: PHAS-I, 4e-bp1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13685
HGNC: HGNC:3288
Homologene: 3021
Tonsl
Name: tonsoku-like, DNA repair protein
Synonyms: 2810439M11Rik, Nfkbil2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72749
HGNC: HGNC:7801
Homologene: 22754
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 4,348,349 bp
  • A to T, chromosome 1 at 52,102,916 bp
  • C to T, chromosome 1 at 120,068,252 bp
  • A to G, chromosome 2 at 21,368,890 bp
  • A to T, chromosome 2 at 26,378,534 bp
  • A to G, chromosome 2 at 32,678,993 bp
  • T to A, chromosome 2 at 83,765,777 bp
  • T to C, chromosome 2 at 129,616,223 bp
  • T to A, chromosome 3 at 87,781,563 bp
  • T to C, chromosome 4 at 19,575,329 bp
  • T to C, chromosome 4 at 42,973,169 bp
  • A to T, chromosome 4 at 84,276,345 bp
  • A to G, chromosome 4 at 115,491,078 bp
  • A to C, chromosome 4 at 118,934,150 bp
  • A to C, chromosome 4 at 125,126,995 bp
  • T to G, chromosome 5 at 86,235,553 bp
  • T to C, chromosome 5 at 91,951,903 bp
  • A to G, chromosome 5 at 149,050,617 bp
  • T to C, chromosome 6 at 58,008,533 bp
  • T to C, chromosome 6 at 100,866,382 bp
  • T to A, chromosome 6 at 126,642,292 bp
  • A to G, chromosome 6 at 147,535,616 bp
  • T to C, chromosome 7 at 3,736,483 bp
  • A to G, chromosome 7 at 4,471,818 bp
  • T to C, chromosome 7 at 127,557,797 bp
  • T to A, chromosome 8 at 27,275,096 bp
  • T to C, chromosome 8 at 31,168,669 bp
  • T to A, chromosome 8 at 75,753,490 bp
  • A to T, chromosome 9 at 54,446,933 bp
  • G to T, chromosome 9 at 98,568,019 bp
  • T to A, chromosome 10 at 83,490,615 bp
  • C to T, chromosome 10 at 93,861,522 bp
  • T to A, chromosome 11 at 5,962,366 bp
  • T to C, chromosome 11 at 33,276,126 bp
  • T to C, chromosome 11 at 65,989,818 bp
  • T to C, chromosome 11 at 69,737,456 bp
  • C to T, chromosome 11 at 103,503,421 bp
  • A to T, chromosome 11 at 115,587,008 bp
  • A to G, chromosome 11 at 121,836,473 bp
  • A to G, chromosome 12 at 71,138,041 bp
  • T to C, chromosome 12 at 72,304,064 bp
  • T to C, chromosome 13 at 8,853,573 bp
  • T to C, chromosome 13 at 48,933,957 bp
  • T to A, chromosome 15 at 35,886,954 bp
  • C to T, chromosome 15 at 76,633,263 bp
  • T to C, chromosome 15 at 77,405,020 bp
  • T to C, chromosome 15 at 82,063,239 bp
  • T to C, chromosome 16 at 16,307,283 bp
  • G to A, chromosome 16 at 17,342,866 bp
  • C to T, chromosome 16 at 20,591,222 bp
  • A to T, chromosome 17 at 32,697,940 bp
  • A to G, chromosome 17 at 53,413,461 bp
  • C to G, chromosome 17 at 71,255,146 bp
  • G to A, chromosome 17 at 74,446,545 bp
  • A to T, chromosome 19 at 10,551,827 bp
  • A to G, chromosome 19 at 13,726,671 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8311 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067796-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.