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Strain Name:
C57BL/6J-MtgxR8313Btlr/Mmmh
Stock Number:
067797-MU
Citation ID:
RRID:MMRRC_067797-MU
Other Names:
R8313 (G1)
Major Collection:

Strain Information

Prkaca
Name: protein kinase, cAMP dependent, catalytic, alpha
Synonyms: Cs, C alpha, PKA, Pkaca
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18747
HGNC: HGNC:9380
Homologene: 121574
Vcp
Name: valosin containing protein
Synonyms: CDC48, p97, p97/VCP, AAA ATPase p97
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269523
Homologene: 5168
Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613
Efemp1
Name: epidermal growth factor-containing fibulin-like extracellular matrix protein 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216616
HGNC: HGNC:3218
Homologene: 3028
Mdk
Name: midkine
Synonyms: MK, Mek
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17242
HGNC: HGNC:6972
Homologene: 1792
Fbxo22
Name: F-box protein 22
Synonyms: 1600016C16Rik, 0610033L19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71999
Homologene: 12433
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 40,580,360 bp
  • G to A, chromosome 1 at 46,175,296 bp
  • A to T, chromosome 1 at 58,921,147 bp
  • A to T, chromosome 1 at 59,108,078 bp
  • T to C, chromosome 2 at 35,727,428 bp
  • A to G, chromosome 2 at 69,945,134 bp
  • T to C, chromosome 2 at 91,930,833 bp
  • T to A, chromosome 2 at 153,989,258 bp
  • G to T, chromosome 3 at 97,663,595 bp
  • T to C, chromosome 4 at 42,988,728 bp
  • G to A, chromosome 4 at 49,665,599 bp
  • G to A, chromosome 4 at 126,910,969 bp
  • G to A, chromosome 4 at 141,002,735 bp
  • A to G, chromosome 5 at 89,046,263 bp
  • T to A, chromosome 5 at 108,643,209 bp
  • A to G, chromosome 5 at 112,875,179 bp
  • G to T, chromosome 5 at 120,609,651 bp
  • A to G, chromosome 6 at 127,689,850 bp
  • T to C, chromosome 7 at 12,193,067 bp
  • A to C, chromosome 7 at 24,260,662 bp
  • A to G, chromosome 7 at 28,086,344 bp
  • A to T, chromosome 7 at 41,872,528 bp
  • A to G, chromosome 7 at 119,951,152 bp
  • T to A, chromosome 7 at 123,170,713 bp
  • A to T, chromosome 8 at 83,990,522 bp
  • A to T, chromosome 8 at 111,713,638 bp
  • A to C, chromosome 9 at 18,525,147 bp
  • T to A, chromosome 9 at 38,368,050 bp
  • A to G, chromosome 9 at 44,436,054 bp
  • T to A, chromosome 9 at 55,221,060 bp
  • G to T, chromosome 9 at 59,668,619 bp
  • A to T, chromosome 9 at 75,625,758 bp
  • A to T, chromosome 9 at 122,853,630 bp
  • T to C, chromosome 9 at 123,127,877 bp
  • C to T, chromosome 10 at 78,879,336 bp
  • T to G, chromosome 10 at 82,225,588 bp
  • T to A, chromosome 10 at 88,439,209 bp
  • T to C, chromosome 10 at 93,563,249 bp
  • T to A, chromosome 11 at 16,281,898 bp
  • A to G, chromosome 11 at 28,910,691 bp
  • C to T, chromosome 11 at 87,872,731 bp
  • T to C, chromosome 11 at 116,804,550 bp
  • T to A, chromosome 12 at 25,004,111 bp
  • G to T, chromosome 12 at 30,884,851 bp
  • A to G, chromosome 12 at 52,024,124 bp
  • A to T, chromosome 12 at 87,876,994 bp
  • G to C, chromosome 13 at 22,772,777 bp
  • T to C, chromosome 13 at 100,624,566 bp
  • T to A, chromosome 13 at 114,966,584 bp
  • A to T, chromosome 14 at 44,724,058 bp
  • AC to A, chromosome 14 at 56,678,605 bp
  • A to G, chromosome 15 at 73,696,312 bp
  • C to A, chromosome 16 at 5,013,449 bp
  • T to C, chromosome 16 at 31,989,299 bp
  • A to T, chromosome 16 at 32,753,423 bp
  • C to A, chromosome 16 at 49,120,018 bp
  • C to T, chromosome 16 at 59,098,641 bp
  • C to T, chromosome 17 at 20,890,027 bp
  • C to A, chromosome 17 at 56,044,109 bp
  • A to G, chromosome 17 at 67,750,520 bp
  • C to T, chromosome 17 at 79,852,604 bp
  • A to C, chromosome 19 at 34,474,738 bp
  • A to G, chromosome 19 at 38,795,810 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8313 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067797-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.