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Strain Name:
C57BL/6J-MtgxR8322Btlr/Mmmh
Stock Number:
067798-MU
Citation ID:
RRID:MMRRC_067798-MU
Other Names:
R8322 (G1)
Major Collection:

Strain Information

B4galt5
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5
Synonyms: 9430078I07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56336
HGNC: HGNC:928
Homologene: 3507
Gli1
Name: GLI-Kruppel family member GLI1
Synonyms: Zfp-5, Zfp5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14632
VEGA: 10
HGNC: HGNC:4317
Homologene: 3859
Mybl1
Name: myeloblastosis oncogene-like 1
Synonyms: A-myb, repro9, G1-419-6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17864
HGNC: HGNC:7547
Homologene: 32045
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Nup62
Name: nucleoporin 62
Synonyms: p62, Nupc1, D7Ertd649e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18226
HGNC: HGNC:8066
Homologene: 68773
Fam169a
Name: family with sequence similarity 169, member A
Synonyms: B230112C05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 320557
VEGA: 13
Homologene: 52656
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 9,676,281 bp
  • A to G, chromosome 1 at 38,181,661 bp
  • T to C, chromosome 1 at 59,483,083 bp
  • T to A, chromosome 1 at 71,628,459 bp
  • A to G, chromosome 1 at 131,977,785 bp
  • G to T, chromosome 1 at 139,810,958 bp
  • A to T, chromosome 1 at 185,246,680 bp
  • T to A, chromosome 1 at 189,339,849 bp
  • T to A, chromosome 2 at 80,624,291 bp
  • G to A, chromosome 2 at 167,348,929 bp
  • T to A, chromosome 3 at 90,073,704 bp
  • T to C, chromosome 3 at 93,284,332 bp
  • C to G, chromosome 4 at 137,518,979 bp
  • T to G, chromosome 4 at 152,104,744 bp
  • A to G, chromosome 5 at 114,402,686 bp
  • A to G, chromosome 5 at 142,726,012 bp
  • A to G, chromosome 6 at 48,765,436 bp
  • C to A, chromosome 6 at 70,878,919 bp
  • C to A, chromosome 6 at 108,388,229 bp
  • T to A, chromosome 6 at 128,713,613 bp
  • A to T, chromosome 7 at 19,971,703 bp
  • G to A, chromosome 7 at 24,384,120 bp
  • T to C, chromosome 7 at 44,829,016 bp
  • T to G, chromosome 7 at 66,789,638 bp
  • A to T, chromosome 7 at 127,473,827 bp
  • C to T, chromosome 7 at 128,290,614 bp
  • T to A, chromosome 7 at 142,385,460 bp
  • A to G, chromosome 8 at 13,078,555 bp
  • A to G, chromosome 8 at 72,445,375 bp
  • A to T, chromosome 8 at 95,769,844 bp
  • T to C, chromosome 8 at 111,045,528 bp
  • G to A, chromosome 9 at 54,615,984 bp
  • T to A, chromosome 9 at 57,702,720 bp
  • T to C, chromosome 9 at 108,068,507 bp
  • T to C, chromosome 9 at 108,848,794 bp
  • T to C, chromosome 9 at 114,627,469 bp
  • A to T, chromosome 10 at 82,662,203 bp
  • C to A, chromosome 10 at 127,331,608 bp
  • T to A, chromosome 11 at 80,257,560 bp
  • T to C, chromosome 11 at 100,712,290 bp
  • G to A, chromosome 11 at 101,432,166 bp
  • A to T, chromosome 11 at 117,868,667 bp
  • T to C, chromosome 11 at 118,447,857 bp
  • T to A, chromosome 12 at 30,588,518 bp
  • C to T, chromosome 12 at 31,933,388 bp
  • T to A, chromosome 12 at 72,556,663 bp
  • T to C, chromosome 12 at 73,939,599 bp
  • T to C, chromosome 13 at 48,491,099 bp
  • A to G, chromosome 13 at 97,122,752 bp
  • A to T, chromosome 14 at 21,702,882 bp
  • A to T, chromosome 14 at 104,479,655 bp
  • G to A, chromosome 15 at 100,010,356 bp
  • T to A, chromosome 16 at 15,714,141 bp
  • A to G, chromosome 16 at 17,357,573 bp
  • A to G, chromosome 16 at 94,454,492 bp
  • A to G, chromosome 17 at 18,166,624 bp
  • A to G, chromosome 17 at 34,193,189 bp
  • A to G, chromosome 17 at 37,225,350 bp
  • A to T, chromosome 17 at 80,408,299 bp
  • A to G, chromosome 17 at 84,740,068 bp
  • C to T, chromosome 18 at 35,622,535 bp
  • G to A, chromosome 18 at 38,281,577 bp
  • A to T, chromosome 18 at 78,102,441 bp
  • T to C, chromosome 19 at 25,378,478 bp
  • T to C, chromosome 19 at 41,880,219 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8322 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067798-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.