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Strain Name:
C57BL/6J-MtgxR8351Btlr/Mmmh
Stock Number:
067804-MU
Citation ID:
RRID:MMRRC_067804-MU
Other Names:
R8351 (G1)
Major Collection:

Strain Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Mras
Name: muscle and microspikes RAS
Synonyms: 2900078C09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17532
HGNC: HGNC:7227
Homologene: 7424
Gmps
Name: guanine monophosphate synthetase
Synonyms: Gm9479
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229363
HGNC: HGNC:4378
Homologene: 68367
Znfx1
Name: zinc finger, NFX1-type containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98999
Homologene: 10877
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,069,060 bp
  • A to T, chromosome 1 at 53,347,013 bp
  • T to C, chromosome 1 at 92,479,938 bp
  • T to A, chromosome 1 at 106,030,932 bp
  • T to C, chromosome 1 at 155,872,034 bp
  • C to T, chromosome 1 at 162,920,605 bp
  • T to C, chromosome 2 at 36,907,137 bp
  • T to C, chromosome 2 at 82,991,895 bp
  • A to T, chromosome 2 at 112,142,061 bp
  • A to G, chromosome 2 at 167,055,655 bp
  • C to T, chromosome 2 at 180,195,608 bp
  • T to A, chromosome 3 at 29,985,370 bp
  • T to C, chromosome 3 at 63,980,194 bp
  • A to T, chromosome 3 at 91,088,364 bp
  • G to A, chromosome 3 at 96,197,257 bp
  • T to C, chromosome 3 at 122,748,479 bp
  • A to T, chromosome 4 at 21,932,046 bp
  • T to A, chromosome 4 at 130,167,583 bp
  • A to G, chromosome 5 at 108,155,797 bp
  • C to T, chromosome 5 at 110,739,334 bp
  • T to A, chromosome 5 at 119,680,776 bp
  • A to T, chromosome 5 at 120,786,943 bp
  • A to G, chromosome 5 at 123,494,265 bp
  • C to A, chromosome 5 at 137,315,475 bp
  • T to C, chromosome 5 at 137,328,448 bp
  • G to A, chromosome 5 at 138,562,926 bp
  • T to G, chromosome 5 at 142,717,829 bp
  • A to G, chromosome 6 at 39,027,916 bp
  • G to A, chromosome 6 at 71,329,793 bp
  • A to G, chromosome 6 at 71,908,641 bp
  • A to C, chromosome 6 at 96,165,674 bp
  • T to A, chromosome 6 at 136,328,544 bp
  • A to T, chromosome 7 at 21,349,672 bp
  • T to A, chromosome 7 at 23,702,532 bp
  • A to G, chromosome 7 at 27,121,869 bp
  • A to G, chromosome 7 at 44,228,986 bp
  • A to G, chromosome 7 at 51,553,878 bp
  • C to T, chromosome 7 at 80,398,722 bp
  • T to C, chromosome 7 at 110,221,898 bp
  • T to C, chromosome 7 at 140,336,605 bp
  • T to C, chromosome 8 at 48,287,872 bp
  • G to A, chromosome 8 at 94,666,879 bp
  • G to A, chromosome 8 at 119,546,184 bp
  • T to C, chromosome 8 at 125,944,733 bp
  • G to A, chromosome 9 at 15,322,906 bp
  • A to T, chromosome 9 at 18,659,885 bp
  • A to T, chromosome 9 at 55,816,804 bp
  • G to T, chromosome 9 at 79,681,412 bp
  • G to A, chromosome 9 at 87,192,850 bp
  • T to C, chromosome 9 at 99,411,495 bp
  • A to G, chromosome 10 at 50,849,597 bp
  • A to T, chromosome 10 at 78,274,252 bp
  • T to C, chromosome 10 at 84,394,117 bp
  • T to G, chromosome 10 at 88,414,486 bp
  • T to C, chromosome 10 at 107,049,404 bp
  • C to T, chromosome 10 at 126,887,538 bp
  • A to G, chromosome 11 at 21,748,931 bp
  • A to G, chromosome 11 at 77,692,355 bp
  • A to G, chromosome 11 at 106,020,236 bp
  • A to G, chromosome 11 at 119,892,639 bp
  • T to C, chromosome 11 at 121,513,309 bp
  • A to G, chromosome 12 at 8,006,356 bp
  • A to G, chromosome 12 at 89,510,643 bp
  • T to C, chromosome 12 at 116,440,027 bp
  • A to G, chromosome 13 at 11,799,832 bp
  • A to G, chromosome 13 at 17,252,558 bp
  • A to T, chromosome 13 at 21,423,380 bp
  • A to T, chromosome 14 at 3,999,379 bp
  • A to G, chromosome 14 at 47,101,431 bp
  • A to G, chromosome 14 at 54,908,594 bp
  • T to C, chromosome 14 at 69,172,027 bp
  • G to T, chromosome 15 at 73,130,890 bp
  • C to A, chromosome 15 at 80,923,490 bp
  • T to C, chromosome 15 at 88,914,435 bp
  • T to C, chromosome 17 at 17,971,174 bp
  • T to A, chromosome 17 at 24,720,360 bp
  • T to C, chromosome 17 at 56,106,861 bp
  • C to T, chromosome 17 at 58,055,692 bp
  • A to G, chromosome 17 at 85,028,496 bp
  • T to C, chromosome 18 at 12,540,613 bp
  • A to G, chromosome 19 at 4,184,745 bp
  • A to G, chromosome 19 at 6,388,087 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8351 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067804-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.