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Strain Name:
C57BL/6J-MtgxR7274Btlr/Mmmh
Stock Number:
067851-MU
Citation ID:
RRID:MMRRC_067851-MU
Other Names:
R7274 (G1)
Major Collection:

Strain Information

Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
1190005I06Rik
Name: RIKEN cDNA 1190005I06 gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68918
Homologene: 18858
Ssc4d
Name: scavenger receptor cysteine rich family, 4 domains
Synonyms: C330016E03Rik, Srcrb4d
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 109267
Homologene: 34137
Ttc39b
Name: tetratricopeptide repeat domain 39B
Synonyms: 9130422G05Rik, 1810054D07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69863
Homologene: 25228
Klhdc4
Name: kelch domain containing 4
Synonyms: G430025P05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234825
Homologene: 69234
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 10,409,299 bp
  • C to A, chromosome 1 at 57,879,513 bp
  • T to C, chromosome 1 at 71,628,113 bp
  • T to A, chromosome 1 at 127,927,512 bp
  • C to T, chromosome 2 at 76,723,588 bp
  • A to G, chromosome 2 at 86,978,523 bp
  • T to C, chromosome 2 at 90,889,541 bp
  • T to C, chromosome 2 at 103,476,890 bp
  • A to G, chromosome 2 at 121,454,324 bp
  • A to T, chromosome 3 at 35,989,856 bp
  • A to T, chromosome 3 at 122,855,246 bp
  • C to T, chromosome 4 at 11,783,174 bp
  • T to A, chromosome 4 at 32,725,944 bp
  • T to C, chromosome 4 at 83,261,851 bp
  • C to A, chromosome 4 at 131,853,778 bp
  • A to T, chromosome 5 at 75,964,700 bp
  • T to C, chromosome 5 at 135,967,956 bp
  • A to G, chromosome 6 at 29,534,040 bp
  • T to A, chromosome 6 at 71,920,516 bp
  • G to A, chromosome 6 at 113,597,890 bp
  • A to G, chromosome 6 at 136,329,398 bp
  • T to C, chromosome 6 at 145,815,569 bp
  • C to A, chromosome 7 at 29,792,618 bp
  • A to G, chromosome 7 at 65,527,652 bp
  • A to G, chromosome 7 at 120,132,391 bp
  • T to A, chromosome 7 at 120,190,519 bp
  • T to C, chromosome 8 at 61,940,108 bp
  • T to A, chromosome 8 at 65,000,481 bp
  • T to C, chromosome 8 at 120,611,266 bp
  • T to C, chromosome 8 at 121,799,658 bp
  • T to G, chromosome 8 at 124,535,519 bp
  • A to G, chromosome 9 at 86,562,597 bp
  • A to T, chromosome 10 at 27,119,980 bp
  • A to T, chromosome 10 at 39,092,299 bp
  • T to C, chromosome 10 at 77,923,555 bp
  • T to A, chromosome 10 at 115,452,505 bp
  • T to C, chromosome 11 at 24,163,985 bp
  • A to T, chromosome 11 at 51,707,255 bp
  • C to T, chromosome 11 at 59,133,227 bp
  • C to T, chromosome 11 at 60,705,986 bp
  • G to T, chromosome 11 at 114,765,455 bp
  • A to G, chromosome 12 at 52,132,420 bp
  • A to G, chromosome 12 at 59,108,119 bp
  • A to T, chromosome 12 at 87,341,606 bp
  • A to T, chromosome 13 at 19,593,288 bp
  • T to A, chromosome 14 at 30,142,643 bp
  • T to A, chromosome 14 at 30,569,145 bp
  • T to A, chromosome 14 at 50,298,422 bp
  • T to A, chromosome 14 at 61,214,081 bp
  • T to A, chromosome 14 at 62,466,852 bp
  • C to A, chromosome 14 at 65,321,267 bp
  • A to G, chromosome 14 at 66,149,226 bp
  • T to C, chromosome 14 at 76,416,714 bp
  • T to A, chromosome 15 at 7,167,059 bp
  • A to T, chromosome 15 at 80,376,822 bp
  • G to A, chromosome 15 at 88,806,707 bp
  • G to A, chromosome 15 at 89,102,970 bp
  • T to C, chromosome 16 at 9,579,122 bp
  • T to C, chromosome 16 at 16,246,929 bp
  • A to G, chromosome 17 at 25,378,837 bp
  • A to G, chromosome 19 at 3,264,951 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7274 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067851-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.