Strain Name:
C57BL/6J-MtgxR8325Btlr/Mmmh
Stock Number:
067857-MU
Citation ID:
RRID:MMRRC_067857-MU
Other Names:
R8325 (G1)
Major Collection:

Strain Information

Met
Name: met proto-oncogene
Synonyms: HGF receptor, Par4, c-Met
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Plin3
Name: perilipin 3
Synonyms: 1300012C15Rik, Tip47, M6prbp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66905
VEGA: 17
Homologene: 4247
Cmtm7
Name: CKLF-like MARVEL transmembrane domain containing 7
Synonyms: Cklfsf7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102545
VEGA: 9
Homologene: 15882
Kif20a
Name: kinesin family member 20A
Synonyms: Rabkinesin-6, Rab6kifl
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19348
VEGA: 18
HGNC: HGNC:9787
Homologene: 38093
Cep290
Name: centrosomal protein 290
Synonyms: Kiaa, b2b1454Clo, b2b1752Clo, Nphp6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Agrn
Name: agrin
Synonyms: nmf380, NMF380, Agrin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Wdr7
Name: WD repeat domain 7
Synonyms: TRAG, TGF-beta resistance associated gene
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 104082
VEGA: 18
Homologene: 11408
Nkapd1
Name: NKAP domain containing 1
Synonyms: LOC270156, AU019823
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270156
Homologene: 41233
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: enaptin165, nesprin-1, A330049M09Rik, C130039F11Rik, SYNE-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Cmas
Name: cytidine monophospho-N-acetylneuraminic acid synthetase
Synonyms: CMP-sialic acid synthetase, D6Bwg0250e, CMP-Neu5Ac synthase
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12764
Homologene: 7670
Dcp1b
Name: decapping mRNA 1B
Synonyms: B930050E02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319618
Homologene: 51881
Camsap1
Name: calmodulin regulated spectrin-associated protein 1
Synonyms: 9530003A05Rik, PRO2405
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227634
Homologene: 19202
Hbs1l
Name: Hbs1-like (S. cerevisiae)
Synonyms: 2810035F15Rik, eRFS
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 56422
VEGA: 10
HGNC: HGNC:4834
Homologene: 68525
Trp53inp1
Name: transformation related protein 53 inducible nuclear protein 1
Synonyms: Teap, Stinp, 2700057G22Rik, SIP18, SIP27, Tp53inp1, SIP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 60599
Homologene: 11065
Dgcr8
Name: DGCR8, microprocessor complex subunit
Synonyms: Gy1, N41, Vo59c07, D16H22S788E, D16H22S1742E, DiGeorge syndrome critical region gene 8, D16Wis2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 94223
HGNC: HGNC:2847
Homologene: 11223
Emc1
Name: ER membrane protein complex subunit 1
Synonyms: C230096C10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230866
Homologene: 9002
Pcolce2
Name: procollagen C-endopeptidase enhancer 2
Synonyms: 2400001O18Rik, Pcpe2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76477
HGNC: HGNC:8739
Homologene: 8357
Nbas
Name: neuroblastoma amplified sequence
Synonyms: 4933425L03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71169
VEGA: 12
Homologene: 41073
Kcng3
Name: potassium voltage-gated channel, subfamily G, member 3
Synonyms: Kv10.1a, KV6.3, Kv10.1b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 225030
VEGA: 17
Homologene: 15168
Sez6l
Name: seizure related 6 homolog like
Synonyms: Acig1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56747
Homologene: 10895
Lcp2
Name: lymphocyte cytosolic protein 2
Synonyms: m1Khoe, twm, SLP-76, SLP76
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16822
HGNC: HGNC:6529
Homologene: 4065
Acr
Name: acrosin prepropeptide
Synonyms: preproacrosin
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11434
VEGA: 15
HGNC: HGNC:126
Homologene: 855
Trpc7
Name: transient receptor potential cation channel, subfamily C, member 7
Synonyms: TRP7, Trrp8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26946
Homologene: 22689
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 672511
Homologene: 45439
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, 4732483H20Rik, 9630020C08Rik, steerin 3, unc53H3, POMFIL1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 260315
Homologene: 56688
Gm7361
Name: predicted gene 7361
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 664837
Homologene: 69402
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 545030
Homologene: 83473
Ap3b2
Name: adaptor-related protein complex 3, beta 2 subunit
Synonyms: Naptb, beta3B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11775
HGNC: HGNC:567
Homologene: 55837
Cadm2
Name: cell adhesion molecule 2
Synonyms: Igsf4d, 2900078E11Rik, A830029E02Rik, Necl3, SynCAM2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239857
Homologene: 17705
Npsr1
Name: neuropeptide S receptor 1
Synonyms: VRR1, Gpr154, PGR14, 9330128H10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319239
Homologene: 45515
Pcdha1
Name: protocadherin alpha 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 116731
HGNC: HGNC:8663
Homologene: 75093
Tbc1d9
Name: TBC1 domain family, member 9
Synonyms: C76116, 4933431N12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71310
Homologene: 57079
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: Adlican2, CMF608, 6530405F15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242050
Homologene: 18712
Mroh1
Name: maestro heat-like repeat family member 1
Synonyms: Heatr7a, D330001F17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223658
Homologene: 44882
Lmod3
Name: leiomodin 3 (fetal)
Synonyms: 5430424A14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320502
HGNC: HGNC:6649
Homologene: 28097
Serpinb1b
Name: serine (or cysteine) peptidase inhibitor, clade B, member 1b
Synonyms: 6330533H24Rik, ovalbumin, EIB
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 282663
HGNC: HGNC:3311
Homologene: 138408
Or9m1b
Name: olfactory receptor family 9 subfamily M member 1B
Synonyms: Olfr1160, GA_x6K02T2Q125-49498697-49497765, MOR173-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258643
Homologene: 74192
Nt5e
Name: 5' nucleotidase, ecto
Synonyms: ecto-5'-nucleotidase, Nt5, 2210401F01Rik, CD73
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23959
HGNC: HGNC:8021
Homologene: 1895
Mss51
Name: MSS51 mitochondrial translational activator
Synonyms: Zmynd17, 4833444M15Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74843
VEGA: 14
Homologene: 27997
Pdhx
Name: pyruvate dehydrogenase complex, component X
Synonyms: Pdx1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27402
Homologene: 55757
Vmn2r91
Name: vomeronasal 2, receptor 91
Synonyms: EG665210
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 665210
Homologene: 115024
Ang2
Name: angiogenin, ribonuclease A family, member 2
Synonyms: Angrp, Rnase5b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 11731
VEGA: 14
HGNC: HGNC:483
Ccdc62
Name: coiled-coil domain containing 62
Synonyms: G1-485-3, repro29, LOC208908
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208908
Homologene: 82466
Repin1
Name: replication initiator 1
Synonyms: AP4, E430037F08Rik, Zfp464
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58887
Homologene: 22810
Apba2
Name: amyloid beta precursor protein binding family A member 2
Synonyms: X11-like, Mint 2, X11L
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11784
HGNC: HGNC:579
Homologene: 4021
Prr36
Name: proline rich 36
Synonyms: BC068157
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73072
Homologene: 136398
Papss1
Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Synonyms: Asapk, SK1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 23971
HGNC: HGNC:8603
Homologene: 81740
Ifi27l2a
Name: interferon, alpha-inducible protein 27 like 2A
Synonyms: Ifi27, 2310061N23Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 76933
Homologene: 137395
Chfr
Name: checkpoint with forkhead and ring finger domains
Synonyms: RNF116, 5730484M20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231600
Homologene: 10078
Usp18
Name: ubiquitin specific peptidase 18
Synonyms: UBP43, 1110058H21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 24110
Homologene: 8047
Vmn1r238
Name: vomeronasal 1 receptor, 238
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100312476
Homologene: 128342
Or6b1
Name: olfactory receptor family 6 subfamily B member 1
Synonyms: GA_x6K02T2P3E9-4722003-4721068, Olfr449, MOR103-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 259067
HGNC: HGNC:8354
Homologene: 17485
Vmn2r29
Name: vomeronasal 2, receptor 29
Synonyms: 6430701C03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76229
Homologene: 113703
Cyb5d2
Name: cytochrome b5 domain containing 2
Synonyms: neuferricin, 9330151E16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192986
Homologene: 14701
Igkv10-96
Name: immunoglobulin kappa variable 10-96
Synonyms: Gm16637
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 692165
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 25,939,363 bp
  • A to T, chromosome 2 at 88,006,193 bp
  • G to T, chromosome 2 at 103,042,252 bp
  • A to T, chromosome 3 at 59,318,533 bp
  • A to G, chromosome 3 at 131,582,611 bp
  • A to G, chromosome 4 at 11,164,561 bp
  • T to A, chromosome 4 at 139,365,210 bp
  • C to T, chromosome 4 at 156,173,662 bp
  • T to C, chromosome 5 at 26,262,156 bp
  • T to A, chromosome 5 at 110,162,763 bp
  • A to T, chromosome 5 at 112,428,116 bp
  • T to C, chromosome 5 at 123,954,385 bp
  • A to G, chromosome 6 at 17,571,672 bp
  • T to C, chromosome 6 at 42,838,190 bp
  • G to T, chromosome 6 at 48,597,345 bp
  • T to C, chromosome 6 at 68,632,104 bp
  • T to C, chromosome 6 at 97,247,418 bp
  • C to T, chromosome 6 at 119,215,436 bp
  • T to C, chromosome 6 at 121,253,810 bp
  • T to C, chromosome 6 at 142,771,339 bp
  • T to C, chromosome 7 at 7,241,942 bp
  • A to T, chromosome 7 at 64,695,982 bp
  • T to C, chromosome 7 at 81,484,489 bp
  • T to C, chromosome 8 at 4,212,982 bp
  • G to A, chromosome 8 at 83,240,038 bp
  • C to T, chromosome 9 at 24,286,822 bp
  • T to C, chromosome 9 at 50,610,308 bp
  • A to G, chromosome 9 at 88,363,562 bp
  • A to T, chromosome 9 at 95,692,920 bp
  • T to C, chromosome 9 at 114,763,347 bp
  • A to T, chromosome 10 at 5,146,257 bp
  • A to G, chromosome 10 at 21,307,649 bp
  • A to C, chromosome 10 at 100,517,808 bp
  • A to G, chromosome 10 at 109,705,603 bp
  • T to A, chromosome 11 at 34,082,394 bp
  • A to G, chromosome 11 at 72,778,825 bp
  • A to G, chromosome 11 at 119,430,445 bp
  • A to G, chromosome 12 at 13,288,795 bp
  • G to A, chromosome 12 at 103,442,885 bp
  • A to T, chromosome 13 at 33,093,601 bp
  • A to G, chromosome 13 at 56,804,711 bp
  • T to C, chromosome 14 at 20,484,703 bp
  • T to A, chromosome 14 at 32,967,487 bp
  • A to G, chromosome 14 at 51,195,503 bp
  • GCCCAGGCCCC to GCC, chromosome 15 at 76,432,215 bp
  • T to C, chromosome 15 at 89,569,751 bp
  • T to C, chromosome 16 at 18,258,285 bp
  • T to A, chromosome 16 at 66,815,450 bp
  • C to A, chromosome 17 at 18,136,363 bp
  • C to T, chromosome 17 at 56,286,268 bp
  • T to C, chromosome 17 at 83,631,578 bp
  • C to T, chromosome 18 at 3,122,529 bp
  • A to G, chromosome 18 at 34,626,922 bp
  • A to T, chromosome 18 at 36,930,814 bp
  • A to G, chromosome 18 at 63,778,464 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8325 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067857-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.