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Strain Name:
C57BL/6J-MtgxR8325Btlr/Mmmh
Stock Number:
067857-MU
Citation ID:
RRID:MMRRC_067857-MU
Other Names:
R8325 (G1)
Major Collection:

Strain Information

Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Plin3
Name: perilipin 3
Synonyms: 1300012C15Rik, Tip47, M6prbp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66905
VEGA: 17
Homologene: 4247
Cmtm7
Name: CKLF-like MARVEL transmembrane domain containing 7
Synonyms: Cklfsf7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102545
VEGA: 9
Homologene: 15882
Kif20a
Name: kinesin family member 20A
Synonyms: Rabkinesin-6, Rab6kifl
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19348
VEGA: 18
HGNC: HGNC:9787
Homologene: 38093
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Wdr7
Name: WD repeat domain 7
Synonyms: TGF-beta resistance associated gene, TRAG
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 104082
VEGA: 18
Homologene: 11408
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 25,939,363 bp
  • A to T, chromosome 2 at 88,006,193 bp
  • G to T, chromosome 2 at 103,042,252 bp
  • A to T, chromosome 3 at 59,318,533 bp
  • A to G, chromosome 3 at 131,582,611 bp
  • A to G, chromosome 4 at 11,164,561 bp
  • T to A, chromosome 4 at 139,365,210 bp
  • C to T, chromosome 4 at 156,173,662 bp
  • T to C, chromosome 5 at 26,262,156 bp
  • T to A, chromosome 5 at 110,162,763 bp
  • A to T, chromosome 5 at 112,428,116 bp
  • T to C, chromosome 5 at 123,954,385 bp
  • A to G, chromosome 6 at 17,571,672 bp
  • T to C, chromosome 6 at 42,838,190 bp
  • G to T, chromosome 6 at 48,597,345 bp
  • T to C, chromosome 6 at 68,632,104 bp
  • T to C, chromosome 6 at 97,247,418 bp
  • C to T, chromosome 6 at 119,215,436 bp
  • T to C, chromosome 6 at 121,253,810 bp
  • T to C, chromosome 6 at 142,771,339 bp
  • T to C, chromosome 7 at 7,241,942 bp
  • A to T, chromosome 7 at 64,695,982 bp
  • T to C, chromosome 7 at 81,484,489 bp
  • T to C, chromosome 8 at 4,212,982 bp
  • G to A, chromosome 8 at 83,240,038 bp
  • C to T, chromosome 9 at 24,286,822 bp
  • T to C, chromosome 9 at 50,610,308 bp
  • A to G, chromosome 9 at 88,363,562 bp
  • A to T, chromosome 9 at 95,692,920 bp
  • T to C, chromosome 9 at 114,763,347 bp
  • A to T, chromosome 10 at 5,146,257 bp
  • A to G, chromosome 10 at 21,307,649 bp
  • A to C, chromosome 10 at 100,517,808 bp
  • A to G, chromosome 10 at 109,705,603 bp
  • T to A, chromosome 11 at 34,082,394 bp
  • A to G, chromosome 11 at 72,778,825 bp
  • A to G, chromosome 11 at 119,430,445 bp
  • A to G, chromosome 12 at 13,288,795 bp
  • G to A, chromosome 12 at 103,442,885 bp
  • A to T, chromosome 13 at 33,093,601 bp
  • A to G, chromosome 13 at 56,804,711 bp
  • T to C, chromosome 14 at 20,484,703 bp
  • T to A, chromosome 14 at 32,967,487 bp
  • A to G, chromosome 14 at 51,195,503 bp
  • GCCCAGGCCCC to GCC, chromosome 15 at 76,432,215 bp
  • T to C, chromosome 15 at 89,569,751 bp
  • T to C, chromosome 16 at 18,258,285 bp
  • T to A, chromosome 16 at 66,815,450 bp
  • C to A, chromosome 17 at 18,136,363 bp
  • C to T, chromosome 17 at 56,286,268 bp
  • T to C, chromosome 17 at 83,631,578 bp
  • C to T, chromosome 18 at 3,122,529 bp
  • A to G, chromosome 18 at 34,626,922 bp
  • A to T, chromosome 18 at 36,930,814 bp
  • A to G, chromosome 18 at 63,778,464 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8325 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067857-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.