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Strain Name:
C57BL/6J-MtgxR8331Btlr/Mmmh
Stock Number:
067860-MU
Citation ID:
RRID:MMRRC_067860-MU
Other Names:
R8331 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Fis1
Name: fission, mitochondrial 1
Synonyms: 2010003O14Rik, Ttc11
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66437
Homologene: 41099
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, 4930432B04Rik, D730027C18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75786
Homologene: 8844
Dido1
Name: death inducer-obliterator 1
Synonyms: DIO-1, 6720461J16Rik, D130048F08Rik, Datf1, dido, C130092D22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 23856
HGNC: HGNC:2680
Homologene: 34139
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 120,241,488 bp
  • A to T, chromosome 1 at 191,046,211 bp
  • T to C, chromosome 2 at 13,340,242 bp
  • T to A, chromosome 2 at 21,212,174 bp
  • T to C, chromosome 2 at 34,948,358 bp
  • T to C, chromosome 2 at 76,880,138 bp
  • T to A, chromosome 2 at 91,576,200 bp
  • A to G, chromosome 2 at 111,664,382 bp
  • T to A, chromosome 2 at 155,990,253 bp
  • A to T, chromosome 2 at 168,675,297 bp
  • A to T, chromosome 2 at 180,660,449 bp
  • T to A, chromosome 2 at 180,996,766 bp
  • G to A, chromosome 3 at 94,996,684 bp
  • C to A, chromosome 4 at 109,066,181 bp
  • C to T, chromosome 4 at 118,226,066 bp
  • C to T, chromosome 4 at 128,712,194 bp
  • C to A, chromosome 4 at 129,639,486 bp
  • T to A, chromosome 5 at 65,807,600 bp
  • T to C, chromosome 5 at 97,045,069 bp
  • A to G, chromosome 5 at 113,550,935 bp
  • T to C, chromosome 5 at 136,963,133 bp
  • A to T, chromosome 5 at 146,525,949 bp
  • A to G, chromosome 6 at 12,471,158 bp
  • T to C, chromosome 6 at 38,148,474 bp
  • C to T, chromosome 6 at 71,976,179 bp
  • T to C, chromosome 6 at 73,025,000 bp
  • C to T, chromosome 6 at 91,053,666 bp
  • T to C, chromosome 6 at 116,552,362 bp
  • T to C, chromosome 6 at 118,630,329 bp
  • T to C, chromosome 6 at 119,953,833 bp
  • T to A, chromosome 6 at 124,061,928 bp
  • A to G, chromosome 7 at 4,549,481 bp
  • C to T, chromosome 7 at 6,317,555 bp
  • G to A, chromosome 7 at 44,211,575 bp
  • C to T, chromosome 7 at 45,000,959 bp
  • C to T, chromosome 7 at 49,452,623 bp
  • A to G, chromosome 7 at 88,297,120 bp
  • C to A, chromosome 7 at 113,313,496 bp
  • G to A, chromosome 7 at 122,221,910 bp
  • T to A, chromosome 7 at 142,201,826 bp
  • A to T, chromosome 8 at 11,413,985 bp
  • G to A, chromosome 8 at 45,315,708 bp
  • C to T, chromosome 9 at 21,991,711 bp
  • A to G, chromosome 9 at 38,292,085 bp
  • T to G, chromosome 10 at 12,614,619 bp
  • T to C, chromosome 10 at 43,876,525 bp
  • A to G, chromosome 11 at 32,588,928 bp
  • A to T, chromosome 11 at 52,026,877 bp
  • A to T, chromosome 11 at 70,321,097 bp
  • C to T, chromosome 11 at 102,011,715 bp
  • A to G, chromosome 12 at 8,001,882 bp
  • T to A, chromosome 12 at 64,473,468 bp
  • C to A, chromosome 12 at 84,603,952 bp
  • A to G, chromosome 13 at 9,695,154 bp
  • A to G, chromosome 13 at 19,658,866 bp
  • G to T, chromosome 13 at 62,700,903 bp
  • A to T, chromosome 14 at 47,272,245 bp
  • G to T, chromosome 14 at 55,071,987 bp
  • A to G, chromosome 14 at 63,818,943 bp
  • A to T, chromosome 15 at 34,428,681 bp
  • T to C, chromosome 15 at 42,676,257 bp
  • T to C, chromosome 15 at 81,601,210 bp
  • T to C, chromosome 15 at 89,084,928 bp
  • T to A, chromosome 15 at 102,502,331 bp
  • A to C, chromosome 16 at 21,947,366 bp
  • A to G, chromosome 17 at 8,566,707 bp
  • A to G, chromosome 17 at 25,167,953 bp
  • T to A, chromosome 17 at 34,921,569 bp
  • G to A, chromosome 17 at 71,729,226 bp
  • T to C, chromosome 18 at 10,458,706 bp
  • T to C, chromosome 18 at 32,240,818 bp
  • T to C, chromosome 18 at 37,437,289 bp
  • C to A, chromosome 19 at 6,313,447 bp
  • C to G, chromosome 19 at 6,352,823 bp
  • G to A, chromosome 19 at 40,230,551 bp
  • A to T, chromosome 19 at 46,709,006 bp
  • T to A, chromosome 19 at 56,440,965 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8331 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067860-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.