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Strain Name:
C57BL/6J-MtgxR8331Btlr/Mmmh
Stock Number:
067860-MU
Citation ID:
RRID:MMRRC_067860-MU
Other Names:
R8331 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Fis1
Name: fission, mitochondrial 1
Synonyms: 2010003O14Rik, Ttc11
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66437
Homologene: 41099
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, 4930432B04Rik, D730027C18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75786
Homologene: 8844
Dido1
Name: death inducer-obliterator 1
Synonyms: DIO-1, 6720461J16Rik, D130048F08Rik, Datf1, dido, C130092D22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 23856
HGNC: HGNC:2680
Homologene: 34139
Txlna
Name: taxilin alpha
Synonyms: 2600010N21Rik, Txln, IL14
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109658
Homologene: 14062
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, Rainb1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78286
Homologene: 52330
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: mRPA1, RPA194, 194kDa, 3010014K16Rik, Rpo1-4, 2900087K15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20019
Homologene: 7033
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Bmal1
Name: basic helix-loop-helix ARNT like 1
Synonyms: Arnt3, MOP3, bHLHe5, Arntl
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11865
HGNC: HGNC:701
Homologene: 910
N4bp2
Name: NEDD4 binding protein 2
Synonyms: LOC333789, LOC386488, B3bp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 333789
Homologene: 32396
Cdc42bpg
Name: CDC42 binding protein kinase gamma
Synonyms: MRCKgamma
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240505
Homologene: 28384
Map3k12
Name: mitogen-activated protein kinase kinase kinase 12
Synonyms: Zpk, DLK, MUK
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 26404
HGNC: HGNC:6851
Homologene: 4592
Apob
Name: apolipoprotein B
Synonyms: apob-100, apob-48
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Platr25
Name: pluripotency associated transcript 25
Synonyms: Gm5665, 6720490N10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 435366
Osbpl9
Name: oxysterol binding protein-like 9
Synonyms: 2600011I06Rik, ORP-9
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100273
Homologene: 69380
Mpp3
Name: membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
Synonyms: Dlgh3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13384
HGNC: HGNC:7221
Homologene: 31065
Ctsc
Name: cathepsin C
Synonyms: DPP1, dipeptidylpeptidase 1, DPPI
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13032
HGNC: HGNC:2528
Homologene: 1373
Pi4kb
Name: phosphatidylinositol 4-kinase beta
Synonyms: ESTM41, Pik4cb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 107650
HGNC: HGNC:8984
Homologene: 6741
Pdlim1
Name: PDZ and LIM domain 1 (elfin)
Synonyms: mClim1, CLP36
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54132
HGNC: HGNC:2067
Homologene: 9643
Zmynd11
Name: zinc finger, MYND domain containing 11
Synonyms: 2210402G22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66505
Homologene: 4828
Zfhx2
Name: zinc finger homeobox 2
Synonyms: zfh-5
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239102
Homologene: 52657
Slc44a4
Name: solute carrier family 44, member 4
Synonyms: NG22, 2210409B01Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70129
Homologene: 11359
Tmem41a
Name: transmembrane protein 41a
Synonyms: 2900010K02Rik, 5730578N08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66664
VEGA: 16
Homologene: 41643
Steap3
Name: STEAP family member 3
Synonyms: pHyde, 1010001D01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68428
Homologene: 10084
Tatdn3
Name: TatD DNase domain containing 3
Synonyms: 1500010M24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68972
Homologene: 45949
Cubn
Name: cubilin
Synonyms: D2Wsu88e, intrinsic factor-cobalamin receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Alox12e
Name: arachidonate lipoxygenase, epidermal
Synonyms: 8-LOX, e-LOX1, Alox12-ps1, Aloxe, Alox12-ps2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11685
Homologene: 105868
Cacna1c
Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms: (alpha)1 subunit, Cchl1a1, Cav1.2, L-type Cav1.2, D930026N18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12288
HGNC: HGNC:1390
Homologene: 55484
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Chp2
Name: calcineurin-like EF hand protein 2
Synonyms: Cbhp2, 2010110P09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70261
Homologene: 11131
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Stk10
Name: serine/threonine kinase 10
Synonyms: Lok, Gek1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20868
Homologene: 38122
Ptprh
Name: protein tyrosine phosphatase receptor type H
Synonyms: SAP-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545902
HGNC: HGNC:9672
Homologene: 37693
Vmn2r26
Name: vomeronasal 2, receptor 26
Synonyms: V2r1b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56552
Homologene: 135915
Fscb
Name: fibrous sheath CABYR binding protein
Synonyms: EG623046
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 623046
VEGA: 12
Homologene: 136275
Col4a2
Name: collagen, type IV, alpha 2
Synonyms: Col4a-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12827
HGNC: HGNC:2203
Homologene: 1390
Thsd7a
Name: thrombospondin, type I, domain containing 7A
Synonyms: LOC330267
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330267
Homologene: 46582
Greb1l
Name: growth regulation by estrogen in breast cancer-like
Synonyms: mKIAA4095, AK220484
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 381157
Homologene: 73393
Nup210
Name: nucleoporin 210
Synonyms: gp210, gp190, Pom210
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54563
Homologene: 41286
Abcd4
Name: ATP-binding cassette, sub-family D member 4
Synonyms: P69r, Pxmp1l
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19300
VEGA: 12
HGNC: HGNC:68
Homologene: 3703
Atp9a
Name: ATPase, class II, type 9A
Synonyms: Class II, IIa
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11981
Homologene: 69194
Odad3
Name: outer dynein arm docking complex subunit 3
Synonyms: Ccdc151, C330001K17Rik, b2b1885Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77609
VEGA: 9
Homologene: 16533
Matn2
Name: matrilin 2
Synonyms: Crtm2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17181
VEGA: 15
HGNC: HGNC:6908
Homologene: 20538
Nme8
Name: NME/NM23 family member 8
Synonyms: 1700056P15Rik, Sptrx-2, Txndc3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73412
Homologene: 9593
Xkr6
Name: X-linked Kx blood group related 6
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219149
Homologene: 18287
Wdhd1
Name: WD repeat and HMG-box DNA binding protein 1
Synonyms: AND-1, D630024B06Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218973
Homologene: 56019
Col20a1
Name: collagen, type XX, alpha 1
Synonyms: 1700051I12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 73368
Homologene: 75138
Togaram2
Name: TOG array regulator of axonemal microtubules 2
Synonyms: 4632412N22Rik, Fam179a
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320159
Homologene: 65315
Traf1
Name: TNF receptor-associated factor 1
Synonyms: 4732496E14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22029
Homologene: 4138
Angpt1
Name: angiopoietin 1
Synonyms: Ang-1, Angiopoietin-1, 1110046O21Rik, ang1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11600
VEGA: 15
HGNC: HGNC:484
Homologene: 37447
Pcdhb12
Name: protocadherin beta 12
Synonyms: Pcdhb5F, PcdhbL, Pcdh3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93883
HGNC: HGNC:8690
Homologene: 87126
Klk1b4
Name: kallikrein 1-related pepidase b4
Synonyms: mGk-4, Ngfa, Ngfa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18048
Homologene: 68141
Ccdc154
Name: coiled-coil domain containing 154
Synonyms: LOC207209, ntl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 207209
Homologene: 19361
Bmp2k
Name: BMP2 inducible kinase
Synonyms: BIKE, 4933417M22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 140780
Homologene: 34134
Qrsl1
Name: glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1
Synonyms: GatA, 2700038P16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76563
Homologene: 6699
Ercc3
Name: excision repair cross-complementing rodent repair deficiency, complementation group 3
Synonyms: XPB
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13872
HGNC: HGNC:3435
Homologene: 96
Trabd
Name: TraB domain containing
Synonyms: 5730502D15Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67976
Homologene: 36427
Cep250
Name: centrosomal protein 250
Synonyms: Inmp, B230210E21Rik, Cep2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16328
HGNC: HGNC:1859
Homologene: 38286
Wscd2
Name: WSC domain containing 2
Synonyms: 4933413A10Rik, C530024P05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320916
Homologene: 35372
Phc2
Name: polyhomeotic 2
Synonyms: Mph2, D4Ertd810e, Edr2, D130050K19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 54383
HGNC: HGNC:3183
Homologene: 75090
Or4k49
Name: olfactory receptor family 4 subfamily K member 49
Synonyms: GA_x6K02T2Q125-72715642-72716580, MOR248-8, Olfr1299
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258886
Homologene: 17426
Thnsl1
Name: threonine synthase-like 1 (bacterial)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 208967
Homologene: 32609
Map4k2
Name: mitogen-activated protein kinase kinase kinase kinase 2
Synonyms: BL44, Rab8ip
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 26412
HGNC: HGNC:6864
Homologene: 3370
Cyp4v3
Name: cytochrome P450, family 4, subfamily v, polypeptide 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102294
Homologene: 133054
Irf3
Name: interferon regulatory factor 3
Synonyms: C920001K05Rik, IRF-3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54131
HGNC: HGNC:6118
Homologene: 1208
Casp7
Name: caspase 7
Synonyms: CMH-1, ICE-IAP3, Mch3, caspase-7
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12369
VEGA: 19
HGNC: HGNC:1508
Homologene: 11168
Zfp583
Name: zinc finger protein 583
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 213011
Homologene: 65048
As3mt
Name: arsenite methyltransferase
Synonyms: Cyt19, 2310045H08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 57344
VEGA: 19
Homologene: 10754
Krtap5-3
Name: keratin associated protein 5-3
Synonyms: A030007E19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77215
Or8c18
Name: olfactory receptor family 8 subfamily C member 18
Synonyms: GA_x6K02T2PVTD-31985209-31986136, MOR170-10P, Olfr896
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258030
VEGA: 9
Eif4a3l2
Name: eukaryotic translation initiation factor 4A3 like 2
Synonyms: Gm5580
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 434080
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 120,241,488 bp
  • A to T, chromosome 1 at 191,046,211 bp
  • T to C, chromosome 2 at 13,340,242 bp
  • T to A, chromosome 2 at 21,212,174 bp
  • T to C, chromosome 2 at 34,948,358 bp
  • T to C, chromosome 2 at 76,880,138 bp
  • T to A, chromosome 2 at 91,576,200 bp
  • A to G, chromosome 2 at 111,664,382 bp
  • T to A, chromosome 2 at 155,990,253 bp
  • A to T, chromosome 2 at 168,675,297 bp
  • A to T, chromosome 2 at 180,660,449 bp
  • T to A, chromosome 2 at 180,996,766 bp
  • G to A, chromosome 3 at 94,996,684 bp
  • C to A, chromosome 4 at 109,066,181 bp
  • C to T, chromosome 4 at 118,226,066 bp
  • C to T, chromosome 4 at 128,712,194 bp
  • C to A, chromosome 4 at 129,639,486 bp
  • T to A, chromosome 5 at 65,807,600 bp
  • T to C, chromosome 5 at 97,045,069 bp
  • A to G, chromosome 5 at 113,550,935 bp
  • T to C, chromosome 5 at 136,963,133 bp
  • A to T, chromosome 5 at 146,525,949 bp
  • A to G, chromosome 6 at 12,471,158 bp
  • T to C, chromosome 6 at 38,148,474 bp
  • C to T, chromosome 6 at 71,976,179 bp
  • T to C, chromosome 6 at 73,025,000 bp
  • C to T, chromosome 6 at 91,053,666 bp
  • T to C, chromosome 6 at 116,552,362 bp
  • T to C, chromosome 6 at 118,630,329 bp
  • T to C, chromosome 6 at 119,953,833 bp
  • T to A, chromosome 6 at 124,061,928 bp
  • A to G, chromosome 7 at 4,549,481 bp
  • C to T, chromosome 7 at 6,317,555 bp
  • G to A, chromosome 7 at 44,211,575 bp
  • C to T, chromosome 7 at 45,000,959 bp
  • C to T, chromosome 7 at 49,452,623 bp
  • A to G, chromosome 7 at 88,297,120 bp
  • C to A, chromosome 7 at 113,313,496 bp
  • G to A, chromosome 7 at 122,221,910 bp
  • T to A, chromosome 7 at 142,201,826 bp
  • A to T, chromosome 8 at 11,413,985 bp
  • G to A, chromosome 8 at 45,315,708 bp
  • C to T, chromosome 9 at 21,991,711 bp
  • A to G, chromosome 9 at 38,292,085 bp
  • T to G, chromosome 10 at 12,614,619 bp
  • T to C, chromosome 10 at 43,876,525 bp
  • A to G, chromosome 11 at 32,588,928 bp
  • A to T, chromosome 11 at 52,026,877 bp
  • A to T, chromosome 11 at 70,321,097 bp
  • C to T, chromosome 11 at 102,011,715 bp
  • A to G, chromosome 12 at 8,001,882 bp
  • T to A, chromosome 12 at 64,473,468 bp
  • C to A, chromosome 12 at 84,603,952 bp
  • A to G, chromosome 13 at 9,695,154 bp
  • A to G, chromosome 13 at 19,658,866 bp
  • G to T, chromosome 13 at 62,700,903 bp
  • A to T, chromosome 14 at 47,272,245 bp
  • G to T, chromosome 14 at 55,071,987 bp
  • A to G, chromosome 14 at 63,818,943 bp
  • A to T, chromosome 15 at 34,428,681 bp
  • T to C, chromosome 15 at 42,676,257 bp
  • T to C, chromosome 15 at 81,601,210 bp
  • T to C, chromosome 15 at 89,084,928 bp
  • T to A, chromosome 15 at 102,502,331 bp
  • A to C, chromosome 16 at 21,947,366 bp
  • A to G, chromosome 17 at 8,566,707 bp
  • A to G, chromosome 17 at 25,167,953 bp
  • T to A, chromosome 17 at 34,921,569 bp
  • G to A, chromosome 17 at 71,729,226 bp
  • T to C, chromosome 18 at 10,458,706 bp
  • T to C, chromosome 18 at 32,240,818 bp
  • T to C, chromosome 18 at 37,437,289 bp
  • C to A, chromosome 19 at 6,313,447 bp
  • C to G, chromosome 19 at 6,352,823 bp
  • G to A, chromosome 19 at 40,230,551 bp
  • A to T, chromosome 19 at 46,709,006 bp
  • T to A, chromosome 19 at 56,440,965 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8331 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067860-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.