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Strain Name:
C57BL/6J-MtgxR8359Btlr/Mmmh
Stock Number:
067872-MU
Citation ID:
RRID:MMRRC_067872-MU
Other Names:
R8359 (G1)
Major Collection:

Strain Information

Ide
Name: insulin degrading enzyme
Synonyms: 1300012G03Rik, 4833415K22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15925
HGNC: HGNC:5381
Homologene: 3645
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Pkp4
Name: plakophilin 4
Synonyms: p0071, Armrp, 5031422I09Rik, 9430019K17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227937
HGNC: HGNC:9026
Homologene: 2689
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 136,116,061 bp
  • T to A, chromosome 1 at 170,283,124 bp
  • A to G, chromosome 2 at 58,777,943 bp
  • A to G, chromosome 2 at 59,350,551 bp
  • T to A, chromosome 2 at 60,443,283 bp
  • A to T, chromosome 2 at 88,652,988 bp
  • A to T, chromosome 2 at 121,363,050 bp
  • A to T, chromosome 2 at 121,867,151 bp
  • G to A, chromosome 2 at 142,711,857 bp
  • A to G, chromosome 2 at 155,249,950 bp
  • G to T, chromosome 2 at 158,761,375 bp
  • A to T, chromosome 2 at 164,356,055 bp
  • C to T, chromosome 3 at 35,964,344 bp
  • T to A, chromosome 3 at 102,820,593 bp
  • T to A, chromosome 3 at 152,248,361 bp
  • A to G, chromosome 4 at 3,871,097 bp
  • C to T, chromosome 4 at 58,957,230 bp
  • T to A, chromosome 4 at 125,063,667 bp
  • A to T, chromosome 4 at 133,420,616 bp
  • T to C, chromosome 4 at 134,349,379 bp
  • T to C, chromosome 5 at 17,653,728 bp
  • A to G, chromosome 5 at 73,075,933 bp
  • T to A, chromosome 5 at 115,984,544 bp
  • C to T, chromosome 6 at 108,846,813 bp
  • T to C, chromosome 6 at 119,992,447 bp
  • C to T, chromosome 7 at 24,104,912 bp
  • T to A, chromosome 7 at 108,668,311 bp
  • T to C, chromosome 7 at 125,868,851 bp
  • A to T, chromosome 8 at 24,806,486 bp
  • A to G, chromosome 8 at 25,088,391 bp
  • A to C, chromosome 8 at 109,606,406 bp
  • G to A, chromosome 9 at 21,569,469 bp
  • A to C, chromosome 9 at 48,947,296 bp
  • T to C, chromosome 9 at 106,068,384 bp
  • A to G, chromosome 9 at 110,862,302 bp
  • T to A, chromosome 10 at 30,198,488 bp
  • T to C, chromosome 10 at 43,992,542 bp
  • C to T, chromosome 10 at 81,242,819 bp
  • G to T, chromosome 11 at 58,552,203 bp
  • T to C, chromosome 11 at 60,407,303 bp
  • T to A, chromosome 13 at 73,544,883 bp
  • T to A, chromosome 14 at 20,666,140 bp
  • T to C, chromosome 15 at 79,287,170 bp
  • T to C, chromosome 15 at 80,474,155 bp
  • C to T, chromosome 16 at 14,208,231 bp
  • C to A, chromosome 16 at 35,824,996 bp
  • T to C, chromosome 16 at 96,016,209 bp
  • A to G, chromosome 17 at 12,683,861 bp
  • G to A, chromosome 17 at 33,176,921 bp
  • A to G, chromosome 17 at 34,802,131 bp
  • A to T, chromosome 17 at 43,310,395 bp
  • A to T, chromosome 17 at 71,431,243 bp
  • A to T, chromosome 17 at 79,628,151 bp
  • A to G, chromosome 18 at 50,281,610 bp
  • T to C, chromosome 19 at 5,971,526 bp
  • A to C, chromosome 19 at 23,234,079 bp
  • A to T, chromosome 19 at 37,330,487 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8359 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067872-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.