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Strain Name:
C57BL/6J-MtgxR8474Btlr/Mmmh
Stock Number:
067918-MU
Citation ID:
RRID:MMRRC_067918-MU
Other Names:
R8474 (G1)
Major Collection:

Strain Information

Qki
Name: quaking, KH domain containing RNA binding
Synonyms: l(17)-1Wis, QkI, l17Wis1, 1110003F05Rik, Qk
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19317
VEGA: 17
Homologene: 11059
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Mindy3
Name: MINDY lysine 48 deubiquitinase 3
Synonyms: 1810041E18Rik, 5830410F13Rik, 2310047O13Rik, Fam188a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66960
Homologene: 11478
Pcif1
Name: phosphorylated CTD interacting factor 1
Synonyms: 2310022K11Rik, F730014I05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228866
Homologene: 11134
Adgrg1
Name: adhesion G protein-coupled receptor G1
Synonyms: Cyt28, Gpr56
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14766
HGNC: HGNC:4512
Homologene: 4156
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382034
Homologene: 40964
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,169,185 bp
  • G to A, chromosome 1 at 173,729,039 bp
  • A to G, chromosome 1 at 184,919,586 bp
  • T to C, chromosome 2 at 12,400,028 bp
  • T to A, chromosome 2 at 23,162,572 bp
  • T to C, chromosome 2 at 112,155,975 bp
  • C to G, chromosome 2 at 146,424,830 bp
  • A to T, chromosome 2 at 164,888,352 bp
  • A to T, chromosome 3 at 72,929,397 bp
  • A to T, chromosome 3 at 100,027,270 bp
  • C to T, chromosome 3 at 129,319,427 bp
  • T to A, chromosome 3 at 145,005,031 bp
  • A to G, chromosome 4 at 43,498,015 bp
  • A to G, chromosome 5 at 8,993,518 bp
  • C to A, chromosome 5 at 146,104,677 bp
  • A to G, chromosome 6 at 52,225,526 bp
  • T to C, chromosome 6 at 83,153,004 bp
  • T to C, chromosome 6 at 123,704,640 bp
  • T to C, chromosome 6 at 134,423,757 bp
  • A to T, chromosome 7 at 19,760,400 bp
  • A to T, chromosome 7 at 44,950,839 bp
  • A to G, chromosome 7 at 45,587,651 bp
  • G to A, chromosome 7 at 83,609,914 bp
  • T to A, chromosome 7 at 109,707,490 bp
  • T to C, chromosome 8 at 4,210,784 bp
  • C to T, chromosome 8 at 83,668,638 bp
  • C to T, chromosome 8 at 87,472,045 bp
  • C to G, chromosome 8 at 95,003,308 bp
  • C to T, chromosome 8 at 120,568,384 bp
  • A to G, chromosome 9 at 21,465,720 bp
  • T to A, chromosome 9 at 39,839,752 bp
  • A to G, chromosome 9 at 40,375,911 bp
  • A to T, chromosome 10 at 41,232,174 bp
  • A to G, chromosome 10 at 88,921,690 bp
  • A to G, chromosome 10 at 127,539,703 bp
  • C to T, chromosome 11 at 40,734,023 bp
  • A to G, chromosome 11 at 72,447,737 bp
  • A to G, chromosome 11 at 80,670,919 bp
  • A to G, chromosome 11 at 104,188,137 bp
  • A to C, chromosome 12 at 44,902,921 bp
  • A to T, chromosome 13 at 24,417,367 bp
  • A to T, chromosome 13 at 70,604,447 bp
  • T to A, chromosome 14 at 69,847,687 bp
  • A to G, chromosome 15 at 28,247,832 bp
  • A to T, chromosome 15 at 64,704,789 bp
  • G to T, chromosome 16 at 43,889,899 bp
  • C to T, chromosome 16 at 73,948,262 bp
  • A to T, chromosome 17 at 10,318,818 bp
  • A to G, chromosome 17 at 32,188,245 bp
  • A to G, chromosome 17 at 44,608,260 bp
  • C to T, chromosome 17 at 57,149,468 bp
  • T to C, chromosome 18 at 12,974,741 bp
  • A to C, chromosome 18 at 74,770,340 bp
  • T to C, chromosome 19 at 6,251,403 bp
  • T to C, chromosome 19 at 10,207,186 bp
  • A to G, chromosome 19 at 13,027,993 bp
  • A to G, chromosome 19 at 34,594,862 bp
  • G to A, chromosome 19 at 47,897,924 bp
  • A to T, chromosome 19 at 60,779,491 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8474 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067918-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.