Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8485Btlr/Mmmh
Stock Number:
067928-MU
Citation ID:
RRID:MMRRC_067928-MU
Other Names:
R8485 (G1)
Major Collection:

Strain Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1, NFATp, NFAT1-D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Phb1
Name: prohibitin 1
Synonyms: Phb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18673
HGNC: HGNC:8912
Homologene: 1980
Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Rai14
Name: retinoic acid induced 14
Synonyms: 1700020L11Rik, Ankycorbin, Norpeg, 1700008J19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75646
VEGA: 15
Homologene: 9199
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Cacng7
Name: calcium channel, voltage-dependent, gamma subunit 7
Synonyms: TARP gamma 7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 81904
Homologene: 12895
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,523,033 bp
  • T to C, chromosome 1 at 20,960,236 bp
  • A to C, chromosome 1 at 46,680,792 bp
  • A to C, chromosome 1 at 51,779,601 bp
  • T to A, chromosome 1 at 133,625,895 bp
  • T to A, chromosome 1 at 166,164,692 bp
  • T to C, chromosome 2 at 19,293,134 bp
  • T to C, chromosome 2 at 29,710,174 bp
  • T to A, chromosome 2 at 32,212,093 bp
  • A to T, chromosome 2 at 87,608,257 bp
  • T to A, chromosome 2 at 110,667,855 bp
  • A to G, chromosome 2 at 143,955,013 bp
  • A to C, chromosome 2 at 168,590,092 bp
  • T to A, chromosome 3 at 79,481,537 bp
  • A to G, chromosome 3 at 97,158,986 bp
  • C to T, chromosome 4 at 98,737,674 bp
  • C to T, chromosome 5 at 31,480,057 bp
  • A to T, chromosome 5 at 33,882,845 bp
  • T to C, chromosome 5 at 65,427,559 bp
  • G to A, chromosome 5 at 117,251,077 bp
  • T to A, chromosome 6 at 33,921,501 bp
  • A to T, chromosome 6 at 145,171,674 bp
  • A to G, chromosome 7 at 3,366,025 bp
  • G to A, chromosome 7 at 19,388,240 bp
  • A to G, chromosome 7 at 30,466,173 bp
  • A to T, chromosome 7 at 34,219,252 bp
  • G to T, chromosome 7 at 44,467,669 bp
  • C to T, chromosome 7 at 128,112,439 bp
  • G to T, chromosome 8 at 22,772,232 bp
  • C to A, chromosome 8 at 122,630,778 bp
  • T to C, chromosome 8 at 122,857,366 bp
  • G to A, chromosome 9 at 26,767,740 bp
  • T to A, chromosome 9 at 38,047,957 bp
  • C to T, chromosome 9 at 66,514,567 bp
  • T to G, chromosome 9 at 119,128,591 bp
  • T to C, chromosome 10 at 26,846,108 bp
  • G to A, chromosome 10 at 127,558,650 bp
  • G to T, chromosome 11 at 58,920,316 bp
  • G to T, chromosome 11 at 60,716,237 bp
  • A to G, chromosome 11 at 94,511,708 bp
  • T to C, chromosome 11 at 94,514,533 bp
  • A to G, chromosome 11 at 95,675,229 bp
  • G to A, chromosome 11 at 101,627,990 bp
  • G to T, chromosome 11 at 115,429,608 bp
  • T to C, chromosome 13 at 70,738,675 bp
  • T to C, chromosome 13 at 95,660,151 bp
  • G to T, chromosome 13 at 104,804,264 bp
  • C to A, chromosome 13 at 104,804,268 bp
  • G to A, chromosome 14 at 12,439,872 bp
  • T to A, chromosome 14 at 54,724,807 bp
  • A to G, chromosome 15 at 10,575,036 bp
  • A to G, chromosome 15 at 44,560,400 bp
  • A to C, chromosome 15 at 74,548,304 bp
  • G to A, chromosome 16 at 32,052,265 bp
  • C to T, chromosome 17 at 65,983,716 bp
  • T to C, chromosome 18 at 5,064,566 bp
  • T to A, chromosome 18 at 43,243,010 bp
  • T to C, chromosome 18 at 57,941,146 bp
  • G to A, chromosome 19 at 42,041,945 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8485 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067928-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.