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Strain Name:
C57BL/6J-MtgxR8491Btlr/Mmmh
Stock Number:
067933-MU
Citation ID:
RRID:MMRRC_067933-MU
Other Names:
R8491 (G1)
Major Collection:

Strain Information

Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Agap1
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Ggap1, Centg2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 347722
Homologene: 56689
Pigl
Name: phosphatidylinositol glycan anchor biosynthesis, class L
Synonyms: LOC327942
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327942
HGNC: HGNC:8966
Homologene: 3159
Aard
Name: alanine and arginine rich domain containing protein
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239435
Homologene: 17041
Gdnf
Name: glial cell line derived neurotrophic factor
Synonyms: glial cell line-derived neurotrophic factor
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14573
VEGA: 15
HGNC: HGNC:4232
Homologene: 433
Gmps
Name: guanine monophosphate synthetase
Synonyms: Gm9479
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229363
HGNC: HGNC:4378
Homologene: 68367
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,157,225 bp
  • C to G, chromosome 1 at 85,577,214 bp
  • A to G, chromosome 1 at 89,609,572 bp
  • T to G, chromosome 1 at 139,457,695 bp
  • T to C, chromosome 2 at 11,279,524 bp
  • A to G, chromosome 2 at 86,413,755 bp
  • A to G, chromosome 3 at 64,014,358 bp
  • A to G, chromosome 3 at 73,051,259 bp
  • A to C, chromosome 3 at 75,252,515 bp
  • A to G, chromosome 3 at 89,958,369 bp
  • T to C, chromosome 3 at 116,582,579 bp
  • T to A, chromosome 4 at 21,993,319 bp
  • T to C, chromosome 4 at 44,995,090 bp
  • T to C, chromosome 4 at 56,906,261 bp
  • C to A, chromosome 4 at 115,301,453 bp
  • C to T, chromosome 4 at 118,614,931 bp
  • T to C, chromosome 4 at 137,553,719 bp
  • C to A, chromosome 5 at 14,515,230 bp
  • T to A, chromosome 5 at 90,795,230 bp
  • A to T, chromosome 6 at 121,882,729 bp
  • T to C, chromosome 7 at 17,090,512 bp
  • A to C, chromosome 7 at 102,559,482 bp
  • A to G, chromosome 7 at 104,149,375 bp
  • G to A, chromosome 7 at 104,580,035 bp
  • G to T, chromosome 8 at 4,626,215 bp
  • T to G, chromosome 8 at 20,917,119 bp
  • A to T, chromosome 8 at 36,584,846 bp
  • A to G, chromosome 8 at 71,392,397 bp
  • T to C, chromosome 8 at 104,617,049 bp
  • A to G, chromosome 9 at 38,741,180 bp
  • A to G, chromosome 9 at 44,500,768 bp
  • T to C, chromosome 9 at 54,599,679 bp
  • A to G, chromosome 10 at 83,576,123 bp
  • C to A, chromosome 10 at 99,266,219 bp
  • G to A, chromosome 11 at 30,772,161 bp
  • A to G, chromosome 11 at 58,854,296 bp
  • A to G, chromosome 11 at 62,473,467 bp
  • A to G, chromosome 11 at 94,214,289 bp
  • G to T, chromosome 11 at 98,823,151 bp
  • G to T, chromosome 11 at 101,332,216 bp
  • A to T, chromosome 13 at 64,785,343 bp
  • A to T, chromosome 14 at 8,083,925 bp
  • T to A, chromosome 14 at 21,837,770 bp
  • T to C, chromosome 14 at 34,039,783 bp
  • A to G, chromosome 15 at 7,834,791 bp
  • A to T, chromosome 15 at 52,040,312 bp
  • C to T, chromosome 15 at 78,994,126 bp
  • T to A, chromosome 15 at 99,740,950 bp
  • A to G, chromosome 17 at 21,509,359 bp
  • C to A, chromosome 17 at 78,384,771 bp
  • T to C, chromosome 18 at 5,106,678 bp
  • A to G, chromosome 18 at 16,624,718 bp
  • A to G, chromosome 19 at 52,264,370 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8491 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067933-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.