Strain Name:
C57BL/6J-MtgxR8494Btlr/Mmmh
Stock Number:
067936-MU
Citation ID:
RRID:MMRRC_067936-MU
Other Names:
R8494 (G1)
Major Collection:

Strain Information

Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Neurl4
Name: neuralized E3 ubiquitin protein ligase 4
Synonyms: 0610025P10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216860
Homologene: 13029
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Tle1
Name: transducin-like enhancer of split 1
Synonyms: Grg1, Estm14, Tle4l, C230057C06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21885
Homologene: 21058
Fbxo15
Name: F-box protein 15
Synonyms: Fbx15, ecat3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 50764
VEGA: 18
Homologene: 17644
Dennd4c
Name: DENN domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329877
Homologene: 23057
Parg
Name: poly (ADP-ribose) glycohydrolase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 26430
HGNC: HGNC:8605
Homologene: 50532
Lfng
Name: LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: lunatic fringe
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16848
HGNC: HGNC:6560
Homologene: 22475
Syn3
Name: synapsin III
Synonyms: Synapsin IIIa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27204
Homologene: 68320
Mier2
Name: MIER family member 2
Synonyms: 2700087H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70427
Homologene: 18941
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: C330002D13Rik, Coh1, 1810042B05Rik, 2310042E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Mcm9
Name: minichromosome maintenance 9 homologous recombination repair factor
Synonyms: 9030408O17Rik, Mcmdc1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71567
VEGA: 10
Homologene: 13546
Adamtsl1
Name: ADAMTS-like 1
Synonyms: punctin-1, 6720426B09Rik, 5930437A14Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 77739
Homologene: 64642
Akap5
Name: A kinase anchor protein 5
Synonyms: LOC238276, AKAP150, 3526401B18Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238276
HGNC: HGNC:375
Homologene: 15854
Kif7
Name: kinesin family member 7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16576
Homologene: 72555
Kirrel3
Name: kirre like nephrin family adhesion molecule 3
Synonyms: Neph2, 1500010O20Rik, 2900036G11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67703
Homologene: 57050
Canx
Name: calnexin
Synonyms: 1110069N15Rik, CNX
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12330
HGNC: HGNC:1473
Homologene: 1324
Anapc16
Name: anaphase promoting complex subunit 16
Synonyms: 2310005G07Rik, D10Ertd641e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52717
Homologene: 11957
Pcnx3
Name: pecanex homolog 3
Synonyms: Pcnxl3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104401
Homologene: 17000
Cblb
Name: Casitas B-lineage lymphoma b
Synonyms: Cbl-b
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208650
VEGA: 16
HGNC: HGNC:1542
Homologene: 15856
Mst1r
Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Synonyms: STK, Fv-2, Ron, CDw136, friend virus susceptibility 2, PTK8, Fv2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19882
HGNC: HGNC:7381
Homologene: 1835
Smpd3
Name: sphingomyelin phosphodiesterase 3, neutral
Synonyms: nSMase2, 4631433G07Rik, fro, Nsm2, neutral sphingomyelinase II
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 58994
Homologene: 10260
Gpx3
Name: glutathione peroxidase 3
Synonyms: GPx, plasma GPx, extracellular GPx, EGPx
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14778
HGNC: HGNC:4555
Homologene: 20480
Dsg3
Name: desmoglein 3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13512
VEGA: 18
HGNC: HGNC:3050
Homologene: 55513
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1537Clo, b2b1565Clo, b2b1154Clo, b2b1134Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319695
Homologene: 85163
Phactr1
Name: phosphatase and actin regulator 1
Synonyms: Rpel1, 9630030F18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218194
Homologene: 33597
Micall1
Name: microtubule associated monooxygenase, calponin and LIM domain containing -like 1
Synonyms: Mus EST 820961, D15N2e, D15Mit260
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 27008
VEGA: 15
Homologene: 24911
Mybpc1
Name: myosin binding protein C, slow-type
Synonyms: Slow-type C-protein, 8030451F13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 109272
HGNC: HGNC:7549
Homologene: 1846
Kcng1
Name: potassium voltage-gated channel, subfamily G, member 1
Synonyms: OTTMUSG00000016048
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241794
HGNC: HGNC:6248
Homologene: 20515
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209683
Homologene: 41023
Gm1110
Name: predicted gene 1110
Synonyms: LOC382064
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382064
VEGA: 9
Homologene: 78608
Trp53rkb
Name: transformation related protein 53 regulating kinase B
Synonyms: mNori-2p, 4933401B08Rik, Trp53rk, PRPK, 5630401H01Rik, Nori-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76367
Homologene: 6042
Apc2
Name: APC regulator of WNT signaling pathway 2
Synonyms: APCL
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 23805
Homologene: 4299
Or4b12
Name: olfactory receptor family 4 subfamily B member 12
Synonyms: GA_x6K02T2Q125-51620802-51619885, MOR227-5, Olfr1271
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258789
HGNC: HGNC:8290
Crocc2
Name: ciliary rootlet coiled-coil, rootletin family member 2
Synonyms: LOC381284, E030010N08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381284
Homologene: 141152
Gpcpd1
Name: glycerophosphocholine phosphodiesterase 1
Synonyms: 2310032D16Rik, 2310004G06Rik, Prei4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74182
Homologene: 10478
Esrp2
Name: epithelial splicing regulatory protein 2
Synonyms: 9530027K23Rik, Rbm35b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 77411
Homologene: 32617
Adamts14
Name: ADAM metallopeptidase with thrombospondin type 1 motif 14
Synonyms: TS14, Adamts-14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237360
Homologene: 16383
Hadha
Name: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
Synonyms: Mtpa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 97212
HGNC: HGNC:4801
Homologene: 152
Selp
Name: selectin, platelet
Synonyms: Grmp, CD62P, P-selectin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20344
Homologene: 2260
Mief1
Name: mitochondrial elongation factor 1
Synonyms: Smcr7l
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239555
VEGA: 15
Homologene: 10374
Ffar2
Name: free fatty acid receptor 2
Synonyms: Gpr43
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233079
HGNC: HGNC:4501
Homologene: 133911
C1qb
Name: complement component 1, q subcomponent, beta polypeptide
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12260
HGNC: HGNC:1242
Homologene: 418
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 71,288,662 bp
  • T to C, chromosome 1 at 72,658,794 bp
  • A to T, chromosome 1 at 93,217,066 bp
  • T to G, chromosome 1 at 164,130,266 bp
  • A to T, chromosome 2 at 90,265,536 bp
  • A to G, chromosome 2 at 132,544,435 bp
  • A to G, chromosome 2 at 166,795,859 bp
  • A to G, chromosome 2 at 168,263,098 bp
  • T to C, chromosome 4 at 72,125,004 bp
  • T to C, chromosome 4 at 86,321,984 bp
  • C to T, chromosome 4 at 86,841,075 bp
  • T to C, chromosome 4 at 136,880,804 bp
  • T to C, chromosome 5 at 30,142,812 bp
  • A to G, chromosome 5 at 111,235,640 bp
  • G to A, chromosome 5 at 140,613,226 bp
  • G to T, chromosome 6 at 70,672,163 bp
  • A to T, chromosome 7 at 30,819,739 bp
  • A to T, chromosome 7 at 79,710,397 bp
  • C to T, chromosome 8 at 106,134,718 bp
  • T to C, chromosome 8 at 106,255,350 bp
  • T to A, chromosome 9 at 26,880,858 bp
  • A to G, chromosome 9 at 34,991,045 bp
  • T to G, chromosome 9 at 107,914,519 bp
  • G to A, chromosome 10 at 53,625,760 bp
  • A to T, chromosome 10 at 59,996,518 bp
  • T to A, chromosome 10 at 61,202,929 bp
  • T to C, chromosome 10 at 79,541,712 bp
  • G to T, chromosome 10 at 80,314,479 bp
  • A to G, chromosome 10 at 86,354,401 bp
  • C to T, chromosome 10 at 88,526,429 bp
  • T to A, chromosome 11 at 50,311,782 bp
  • G to A, chromosome 11 at 54,903,020 bp
  • A to G, chromosome 11 at 69,911,045 bp
  • A to G, chromosome 12 at 76,329,681 bp
  • T to A, chromosome 13 at 43,096,668 bp
  • T to A, chromosome 14 at 32,209,021 bp
  • T to C, chromosome 14 at 87,037,522 bp
  • A to C, chromosome 15 at 28,345,831 bp
  • T to A, chromosome 15 at 35,422,448 bp
  • T to C, chromosome 15 at 79,120,880 bp
  • T to A, chromosome 15 at 80,249,392 bp
  • T to A, chromosome 16 at 52,204,640 bp
  • T to A, chromosome 18 at 20,540,214 bp
  • T to G, chromosome 18 at 84,964,127 bp
  • C to A, chromosome 19 at 5,675,376 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8494 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067936-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.