Strain Name:
C57BL/6J-MtgxR8514Btlr/Mmmh
Stock Number:
067945-MU
Citation ID:
RRID:MMRRC_067945-MU
Other Names:
R8514 (G1)
Major Collection:

Strain Information

Pank3
Name: pantothenate kinase 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211347
Homologene: 56999
Htr1d
Name: 5-hydroxytryptamine (serotonin) receptor 1D
Synonyms: Htr1db, Gpcr14
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15552
HGNC: HGNC:5289
Homologene: 20240
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, DICE1, Ddx26, 2900075H24Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Fryl
Name: FRY like transcription coactivator
Synonyms: 9030227G01Rik, 2310004H21Rik, 2510002A14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: 3110054G10Rik, CAGH26, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: D5Ertd66e, 2610509D04Rik, Ggtb3, Bwf1, Alfy, Bchs
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Dock9
Name: dedicator of cytokinesis 9
Synonyms: D14Wsu89e, B230309H04Rik, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Golga4
Name: golgin A4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Usp38
Name: ubiquitin specific peptidase 38
Synonyms: 4833420O05Rik, 4631402N15Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74841
Homologene: 12367
Tut4
Name: terminal uridylyl transferase 4
Synonyms: 6030404K05Rik, Zcchc11, Tent3a, 9230115F04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230594
Homologene: 35279
Mdn1
Name: midasin AAA ATPase 1
Synonyms: D4Abb1e, LOC213784, 4833432B22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100019
Homologene: 39689
Rad50
Name: RAD50 double strand break repair protein
Synonyms: Mrell, Rad50l
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19360
HGNC: HGNC:9816
Homologene: 38092
Cip2a
Name: cell proliferation regulating inhibitor of protein phosphatase 2A
Synonyms: C330027C09Rik, Cip2a
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224171
Homologene: 10842
Ubn1
Name: ubinuclein 1
Synonyms: 1110029L11Rik, 2610108L02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 170644
VEGA: 16
Homologene: 9656
Ckap2l
Name: cytoskeleton associated protein 2-like
Synonyms: Radmis, 2610318C08Rik, 2010016H04Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70466
Homologene: 51866
Bcam
Name: basal cell adhesion molecule
Synonyms: 1200005K12Rik, B-CAM, Lu
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57278
HGNC: HGNC:6722
Homologene: 21149
Iqsec3
Name: IQ motif and Sec7 domain 3
Synonyms: synarfGEF, BRAG3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243621
Homologene: 46091
Platr25
Name: pluripotency associated transcript 25
Synonyms: Gm5665, 6720490N10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 435366
Plpp1
Name: phospholipid phosphatase 1
Synonyms: Ppap2a, Lipid phosphate phosphatase 1, mPAP, LPP1, LPP-1, Hpic53
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19012
VEGA: 13
HGNC: HGNC:9228
Homologene: 134049
Rasa3
Name: RAS p21 protein activator 3
Synonyms: hlb381, GAPIII activator 3, GAPIII, Ras GTPase-activating protein III, scat, R-Ras gap
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19414
Homologene: 7217
Emc10
Name: ER membrane protein complex subunit 10
Synonyms: 5430410O10Rik, Mirta22, 2310044H10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69683
Homologene: 18036
Creld1
Name: cysteine-rich with EGF-like domains 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171508
Homologene: 32265
Notch1
Name: notch 1
Synonyms: Motch A, Tan1, lin-12, Mis6, 9930111A19Rik, N1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18128
HGNC: HGNC:7881
Homologene: 32049
Pros1
Name: protein S (alpha)
Synonyms: protein S
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19128
HGNC: HGNC:9456
Homologene: 264
Tcerg1
Name: transcription elongation regulator 1 (CA150)
Synonyms: ca150, 2410022J09Rik, p144, 2900090C16Rik, Taf2s, Fbp28
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 56070
Homologene: 4879
Yeats4
Name: YEATS domain containing 4
Synonyms: GAS41, B230215M10Rik, NuBI-1, 4930573H17Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64050
VEGA: 10
Homologene: 4760
Sdc3
Name: syndecan 3
Synonyms: syn-3, Synd3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20970
Homologene: 7965
Calhm6
Name: calcium homeostasis modulator family member 6
Synonyms: A630077B13Rik, Fam26f
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215900
VEGA: 10
Homologene: 25235
Tecta
Name: tectorin alpha
Synonyms: Tctna, [a]-tectorin
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21683
Homologene: 3955
Vmn2r1
Name: vomeronasal 2, receptor 1
Synonyms: V2r83, EG56544
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56544
Homologene: 84832
Capn1
Name: calpain 1
Synonyms: mu-calpin, Capa1, Capa-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12333
VEGA: 19
HGNC: HGNC:1476
Homologene: 3800
Prg4
Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
Synonyms: MSF, lubricin, SZP, DOL54
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 96875
HGNC: HGNC:9364
Homologene: 137352
Trmt1l
Name: tRNA methyltransferase 1 like
Synonyms: Trm1-like, 1190005F20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98685
Homologene: 12801
Mndal
Name: myeloid nuclear differentiation antigen like
Synonyms: Ifi212
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040462
HGNC: HGNC:5395
Homologene: 115929
Pitrm1
Name: pitrilysin metallepetidase 1
Synonyms: PreP, Ntup1, MP-1, 2310012C15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69617
VEGA: 13
Homologene: 5742
Rtl1
Name: retrotransposon Gaglike 1
Synonyms: Mor1, Mar, Mart1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 353326
VEGA: 12
Homologene: 120261
Gjc3
Name: gap junction protein, gamma 3
Synonyms: Cx29, connexin 29, Gje1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 118446
Homologene: 15399
Ccdc40
Name: coiled-coil domain containing 40
Synonyms: B930008I02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 207607
Homologene: 27890
Vmn2r62
Name: vomeronasal 2, receptor 62
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 546964
Homologene: 104832
Spata31e5
Name: spermatogenesis associated 31 subfamily E member 5
Synonyms: LOC210962, Gm597
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210962
Slc9a9
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 9
Synonyms: Nhe9, 5730527A11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 331004
Homologene: 69753
Vmn1r1
Name: vomeronasal 1 receptor 1
Synonyms: Gm6628
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 625823
Homologene: 83459
Phtf2
Name: putative homeodomain transcription factor 2
Synonyms: 9530062N20Rik, 1110054G21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68770
Homologene: 10713
Rgs18
Name: regulator of G-protein signaling 18
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 64214
Homologene: 11281
Gpr33
Name: G protein-coupled receptor 33
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14762
HGNC: HGNC:4489
Homologene: 7345
Snrpa1
Name: small nuclear ribonucleoprotein polypeptide A'
Synonyms: 1500015N06Rik, U2 snRNP-A'
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68981
Homologene: 2325
Or4a15
Name: olfactory receptor family 4 subfamily A member 15
Synonyms: GA_x6K02T2Q125-50805620-50804676, MOR231-2, Olfr1234
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258975
Homologene: 128156
Micall2
Name: MICAL-like 2
Synonyms: MICAL-L2, A930021H16Rik, Jrab
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231830
Homologene: 85155
Or12e10
Name: olfactory receptor family 12 subfamily E member 10
Synonyms: MOR264-19, Olfr1145, GA_x6K02T2Q125-49311440-49312384
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258317
Homologene: 27123
Glrp1
Name: glutamine repeat protein 1
Synonyms: GRP-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14659
Tex101
Name: testis expressed gene 101
Synonyms: TES101RP, 1700008H15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56746
Homologene: 10555
Nthl1
Name: nth (endonuclease III)-like 1 (E.coli)
Synonyms: Nth1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18207
VEGA: 17
HGNC: HGNC:8028
Homologene: 1897
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 28,778,505 bp
  • GTGCTGCTGCTGCTGCTG to GTGCTGCTGCTGCTG, chromosome 1 at 88,503,320 bp
  • T to A, chromosome 1 at 144,754,027 bp
  • G to A, chromosome 1 at 150,454,645 bp
  • T to C, chromosome 1 at 151,453,991 bp
  • T to C, chromosome 1 at 173,860,192 bp
  • C to T, chromosome 1 at 182,157,573 bp
  • A to G, chromosome 2 at 26,472,169 bp
  • A to T, chromosome 2 at 87,810,710 bp
  • T to C, chromosome 2 at 89,363,229 bp
  • G to T, chromosome 2 at 129,285,868 bp
  • A to G, chromosome 3 at 64,086,521 bp
  • T to A, chromosome 4 at 32,739,857 bp
  • T to A, chromosome 4 at 108,557,357 bp
  • A to G, chromosome 4 at 130,818,761 bp
  • A to G, chromosome 4 at 136,443,339 bp
  • T to C, chromosome 5 at 20,802,032 bp
  • T to A, chromosome 5 at 73,085,356 bp
  • A to T, chromosome 5 at 101,851,353 bp
  • T to A, chromosome 5 at 137,957,510 bp
  • C to A, chromosome 5 at 139,716,222 bp
  • C to T, chromosome 6 at 113,492,869 bp
  • A to G, chromosome 6 at 121,413,562 bp
  • A to G, chromosome 7 at 19,758,541 bp
  • T to A, chromosome 7 at 24,668,532 bp
  • A to G, chromosome 7 at 42,764,568 bp
  • G to T, chromosome 7 at 44,493,222 bp
  • G to A, chromosome 7 at 66,070,633 bp
  • C to T, chromosome 7 at 123,184,215 bp
  • A to C, chromosome 8 at 13,581,322 bp
  • C to T, chromosome 8 at 15,125,153 bp
  • T to A, chromosome 8 at 80,985,717 bp
  • T to C, chromosome 9 at 5,352,735 bp
  • A to G, chromosome 9 at 42,373,110 bp
  • T to C, chromosome 9 at 94,936,365 bp
  • T to C, chromosome 9 at 118,555,796 bp
  • G to A, chromosome 10 at 34,126,403 bp
  • T to C, chromosome 10 at 117,215,755 bp
  • A to G, chromosome 11 at 35,776,359 bp
  • G to A, chromosome 11 at 53,678,939 bp
  • A to G, chromosome 11 at 119,230,633 bp
  • A to G, chromosome 12 at 52,023,398 bp
  • C to T, chromosome 12 at 109,593,873 bp
  • T to C, chromosome 13 at 6,568,786 bp
  • T to C, chromosome 13 at 62,700,772 bp
  • A to T, chromosome 13 at 112,834,928 bp
  • A to G, chromosome 14 at 62,695,717 bp
  • A to T, chromosome 14 at 121,658,787 bp
  • A to G, chromosome 16 at 5,073,399 bp
  • T to C, chromosome 16 at 48,997,447 bp
  • A to G, chromosome 16 at 62,910,109 bp
  • G to A, chromosome 17 at 24,634,115 bp
  • T to A, chromosome 18 at 42,564,122 bp
  • T to C, chromosome 19 at 5,997,824 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8514 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067945-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.