Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8519Btlr/Mmmh
Stock Number:
067946-MU
Citation ID:
RRID:MMRRC_067946-MU
Other Names:
R8519 (G1)
Major Collection:

Strain Information

Uso1
Name: USO1 vesicle docking factor
Synonyms: transcytosis associated protein p115, TAP, Vdp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56041
Homologene: 2754
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Trib2
Name: tribbles pseudokinase 2
Synonyms: TRB2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217410
Homologene: 41445
Adcy9
Name: adenylate cyclase 9
Synonyms: D16Wsu65e, ACtp10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Pcif1
Name: phosphorylated CTD interacting factor 1
Synonyms: 2310022K11Rik, F730014I05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228866
Homologene: 11134
Mrpl39
Name: mitochondrial ribosomal protein L39
Synonyms: MRP-L5, Rpml5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27393
Homologene: 9679
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 36,176,643 bp
  • A to T, chromosome 1 at 43,977,205 bp
  • A to T, chromosome 1 at 87,410,709 bp
  • C to T, chromosome 1 at 153,473,176 bp
  • T to C, chromosome 1 at 166,235,558 bp
  • A to G, chromosome 1 at 174,159,048 bp
  • T to A, chromosome 1 at 194,793,958 bp
  • G to T, chromosome 2 at 92,454,707 bp
  • A to G, chromosome 2 at 150,192,780 bp
  • A to G, chromosome 2 at 164,884,383 bp
  • T to A, chromosome 3 at 15,848,362 bp
  • C to A, chromosome 3 at 30,607,141 bp
  • G to T, chromosome 3 at 88,073,062 bp
  • T to C, chromosome 3 at 94,176,190 bp
  • A to G, chromosome 3 at 97,209,018 bp
  • A to G, chromosome 4 at 41,505,071 bp
  • A to G, chromosome 4 at 58,785,203 bp
  • A to G, chromosome 4 at 96,319,302 bp
  • T to C, chromosome 4 at 139,416,647 bp
  • T to C, chromosome 5 at 8,973,629 bp
  • A to G, chromosome 5 at 86,926,467 bp
  • T to G, chromosome 5 at 92,195,363 bp
  • C to T, chromosome 5 at 121,304,426 bp
  • C to T, chromosome 6 at 38,295,241 bp
  • T to C, chromosome 6 at 48,753,134 bp
  • A to G, chromosome 6 at 68,121,782 bp
  • A to G, chromosome 6 at 93,704,349 bp
  • G to A, chromosome 6 at 119,950,083 bp
  • T to C, chromosome 6 at 122,315,066 bp
  • C to A, chromosome 6 at 136,854,351 bp
  • T to C, chromosome 7 at 3,737,433 bp
  • T to A, chromosome 7 at 5,486,348 bp
  • G to A, chromosome 7 at 43,464,902 bp
  • T to A, chromosome 7 at 43,782,815 bp
  • A to T, chromosome 7 at 86,443,854 bp
  • T to C, chromosome 7 at 118,171,759 bp
  • T to C, chromosome 7 at 131,308,897 bp
  • G to A, chromosome 8 at 26,160,897 bp
  • T to A, chromosome 8 at 41,275,939 bp
  • T to A, chromosome 8 at 68,401,453 bp
  • A to G, chromosome 9 at 35,478,654 bp
  • A to G, chromosome 9 at 57,136,777 bp
  • G to A, chromosome 9 at 90,193,557 bp
  • T to G, chromosome 10 at 22,283,522 bp
  • A to T, chromosome 10 at 22,664,779 bp
  • T to C, chromosome 10 at 61,202,840 bp
  • G to T, chromosome 11 at 49,685,114 bp
  • A to G, chromosome 11 at 58,529,503 bp
  • T to G, chromosome 11 at 58,625,974 bp
  • A to G, chromosome 11 at 105,347,306 bp
  • T to C, chromosome 11 at 107,061,764 bp
  • G to T, chromosome 12 at 15,815,346 bp
  • T to G, chromosome 13 at 58,238,692 bp
  • A to T, chromosome 14 at 47,310,463 bp
  • T to C, chromosome 14 at 49,773,236 bp
  • T to A, chromosome 14 at 50,964,385 bp
  • A to T, chromosome 14 at 75,125,872 bp
  • A to G, chromosome 14 at 79,476,823 bp
  • T to G, chromosome 14 at 118,855,027 bp
  • G to A, chromosome 15 at 28,299,099 bp
  • A to G, chromosome 15 at 102,983,909 bp
  • T to C, chromosome 16 at 4,288,128 bp
  • T to C, chromosome 16 at 18,808,847 bp
  • A to G, chromosome 16 at 84,730,848 bp
  • T to C, chromosome 17 at 23,479,468 bp
  • A to T, chromosome 17 at 53,969,681 bp
  • C to A, chromosome 19 at 16,872,983 bp
  • G to A, chromosome 19 at 40,038,413 bp
  • T to A, chromosome 19 at 44,069,602 bp
  • T to C, chromosome 19 at 47,501,672 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8519 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067946-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.