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Strain Name:
C57BL/6J-MtgxR8523Btlr/Mmmh
Stock Number:
067948-MU
Citation ID:
RRID:MMRRC_067948-MU
Other Names:
R8523 (G1)
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Plekha7
Name: pleckstrin homology domain containing, family A member 7
Synonyms: A430081P20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233765
Homologene: 52172
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75415
Homologene: 23089
Pck1
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: Pck-1, PEPCK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18534
HGNC: HGNC:8724
Homologene: 1944
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain II-B, NMHC-B, SMemb, nonmuscle myosin heavy chain IIB, 9330167F11Rik, 5730504C04Rik, NMHC II-B, Myhn2, Myosin IIB, Myhn-2, myosin IIB, Fltn, Fltn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 9,911,721 bp
  • A to T, chromosome 1 at 18,251,295 bp
  • A to G, chromosome 1 at 36,387,190 bp
  • A to G, chromosome 1 at 37,879,558 bp
  • A to T, chromosome 1 at 44,060,834 bp
  • A to C, chromosome 2 at 22,819,636 bp
  • G to A, chromosome 2 at 26,464,905 bp
  • T to A, chromosome 2 at 76,746,945 bp
  • A to T, chromosome 2 at 76,899,743 bp
  • T to A, chromosome 2 at 85,918,063 bp
  • C to A, chromosome 2 at 173,157,271 bp
  • T to A, chromosome 3 at 28,085,876 bp
  • C to T, chromosome 3 at 33,815,411 bp
  • T to C, chromosome 3 at 64,274,890 bp
  • A to T, chromosome 3 at 83,030,851 bp
  • T to C, chromosome 3 at 104,868,057 bp
  • C to T, chromosome 3 at 157,160,660 bp
  • A to T, chromosome 4 at 71,757,228 bp
  • A to G, chromosome 4 at 88,435,049 bp
  • A to T, chromosome 4 at 94,799,166 bp
  • A to T, chromosome 4 at 135,324,002 bp
  • A to T, chromosome 4 at 135,967,676 bp
  • T to G, chromosome 4 at 155,262,511 bp
  • T to G, chromosome 5 at 8,569,505 bp
  • C to A, chromosome 5 at 22,004,231 bp
  • T to C, chromosome 5 at 31,058,106 bp
  • T to A, chromosome 5 at 38,213,325 bp
  • A to G, chromosome 5 at 145,124,682 bp
  • A to G, chromosome 5 at 149,573,996 bp
  • A to G, chromosome 5 at 150,560,148 bp
  • T to A, chromosome 6 at 42,307,589 bp
  • T to C, chromosome 6 at 73,095,188 bp
  • T to A, chromosome 6 at 111,246,319 bp
  • T to C, chromosome 7 at 5,454,054 bp
  • C to T, chromosome 7 at 23,754,757 bp
  • G to A, chromosome 7 at 75,730,465 bp
  • A to T, chromosome 7 at 103,383,206 bp
  • T to G, chromosome 7 at 116,307,929 bp
  • A to T, chromosome 7 at 126,547,897 bp
  • G to A, chromosome 8 at 86,853,161 bp
  • T to C, chromosome 8 at 105,702,249 bp
  • A to G, chromosome 9 at 20,300,797 bp
  • A to G, chromosome 9 at 44,949,832 bp
  • T to A, chromosome 9 at 66,450,942 bp
  • T to C, chromosome 9 at 105,774,788 bp
  • A to G, chromosome 10 at 75,052,503 bp
  • A to G, chromosome 10 at 77,054,234 bp
  • A to T, chromosome 10 at 109,823,277 bp
  • C to A, chromosome 10 at 127,711,158 bp
  • G to A, chromosome 11 at 6,402,441 bp
  • A to T, chromosome 11 at 8,448,779 bp
  • A to G, chromosome 11 at 50,837,930 bp
  • A to T, chromosome 11 at 68,797,409 bp
  • A to T, chromosome 11 at 78,293,384 bp
  • C to T, chromosome 11 at 83,484,852 bp
  • A to T, chromosome 12 at 33,346,024 bp
  • A to G, chromosome 12 at 65,020,314 bp
  • T to C, chromosome 13 at 58,191,755 bp
  • G to T, chromosome 13 at 65,033,177 bp
  • T to A, chromosome 14 at 26,429,810 bp
  • T to C, chromosome 14 at 50,819,790 bp
  • T to C, chromosome 14 at 53,047,665 bp
  • T to C, chromosome 15 at 12,834,322 bp
  • A to T, chromosome 15 at 34,471,356 bp
  • C to T, chromosome 15 at 39,099,886 bp
  • A to G, chromosome 15 at 96,419,107 bp
  • T to A, chromosome 15 at 97,808,370 bp
  • T to A, chromosome 15 at 99,279,732 bp
  • G to A, chromosome 15 at 102,216,522 bp
  • A to T, chromosome 16 at 19,286,101 bp
  • A to T, chromosome 17 at 23,808,515 bp
  • G to A, chromosome 17 at 58,884,013 bp
  • T to C, chromosome 17 at 65,478,371 bp
  • A to T, chromosome 18 at 6,111,976 bp
  • T to C, chromosome 18 at 31,772,099 bp
  • G to T, chromosome 18 at 34,793,076 bp
  • G to A, chromosome 18 at 36,532,143 bp
  • G to A, chromosome 18 at 52,838,016 bp
  • A to G, chromosome 18 at 63,146,802 bp
  • T to C, chromosome 19 at 6,294,583 bp
  • C to T, chromosome 19 at 6,940,435 bp
  • T to C, chromosome 19 at 9,991,663 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8523 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067948-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.