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Availability & Fees Order this Strain
Conditional-ready allele using targeted substitution in the C1qbp gene (aka p32 gene): an FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette; Cre-mediated recombination deleted exon 3.
The development is based on unspecified ESCs derived from 129S6/SvEvTac. Male chimeric mice were mated to C57BL/6J females and resulted in heterozygous agouti mice. The heterozygous F1 agouti females were mated to B6N.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J (RRID:IMSR_JAX:016226) male to delete the neo cassette. Crossing to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (RRID:IMSR_JAX:003724) was used to knock out the gene. p32 heterozygous (neo-) males were used to initiate five backcrosses to C57BL/6J mice with selected deletion of the p32 allele. Maintenance on C57BL/6J background to N12 thereafter.
Liu Y, Leslie PL, Jin A, Itahana K, Graves LM, Zhang Y. p32 heterozygosity protects against age- and diet-induced obesity by increasing energy expenditure. Sci Rep. 2017 Jul 18;7(1):5754. doi: 10.1038/s41598-017-06209-9. (Medline PMID: 28720899)
Liu Y, Leslie PL, Jin A, Itahana K, Graves LM, Zhang Y. p32 regulates ER stress and lipid homeostasis by down-regulating GCS1 expression. FASEB J. 2018 Jul;32(7):3892-3902. doi: 10.1096/fj.201701004RR. Epub 2018 Feb 20. (Medline PMID: 29465311)
Itahana K, Zhang Y. Mitochondrial p32 is a critical mediator of ARF-induced apoptosis. Cancer Cell. 2008 Jun;13(6):542-53. doi: 10.1016/j.ccr.2008.04.002. (Medline PMID: 18538737)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Jacqueline Quay.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Jacqueline Quay
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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