Strain Detail Sheet
The conditional-ready (floxed model) has no phenotype.
Conditional phenotype: Homozygous deleted mice show perinatal lethality due to a cardiac defect (described in PMID:24586749); fetal livers show a significant reduction in KSL stem/progenitors; newborns show mild leukopenia and thrombocytopenia; and specific deletion in the hematopoietic cells results in bone marrow failure, with complete marrow aplasia and markedly severe peripheral cytopenias and death.LoxP sites were inserted on either side of the first exon coding for the EVI1 protein isoforms (exon containing the initiator methionine). These were inserted via homologous recombination in S1B6A embryonic stem cells derived from
(129S1/SvImJ x C57BL/6J)F1. The neo cassette was deleted in vitro through transient expression of a Cre vector. Positive clones were identified by Southern blot analysis. One of these was chosen and inserted into blastocyst cavities for the development of mice. Subsequent backcrossing to C57BL/6J (N20+) and sib-mating (F20+).
- Cancer
- Developmental Biology
- Hematology
- Internal/Organ
Zhang Y, Stehling-Sun S, Lezon-Geyda K, Juneja SC, Coillard L, Chatterjee G, Wuertzer CA, Camargo F, Perkins AS. PR-domain-containing Mds1-Evi1 is critical for long-term hematopoietic stem cell function. Blood. 2011 Oct 6;118(14):3853-61. doi: 10.1182/blood-2011-02-334680. Epub 2011 Jun 10. (Medline PMCID: 3193263)
Zhang Y, Owens K, Hatem L, Glass CH, Karuppaiah K, Camargo F, Perkins AS. Essential role of PR-domain protein MDS1-EVI1 in MLL-AF9 leukemia. Blood. 2013 Oct 17;122(16):2888-92. doi: 10.1182/blood-2012-08-453662. Epub 2013 Sep 10. (Medline PMCID: 3799001)
Bard-Chapeau EA, Szumska D, Jacob B, Chua BQ, Chatterjee GC, Zhang Y, Ward JM, Urun F, Kinameri E, Vincent SD, Ahmed S, Bhattacharya S, Osato M, Perkins AS, Moore AW, Jenkins NA, Copeland NG. Mice carrying a hypomorphic Evi1 allele are embryonic viable but exhibit severe congenital heart defects. PLoS One. 2014 Feb 27;9(2):e89397. doi: 10.1371/journal.pone.0089397. (Medline PMCID: 3937339)
Colony Surveillance Program and Current Health Reports
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Yes | Yes | |
| Heterozygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Undetermined | Undetermined |
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 068164-UNC-RESUS | Litter recovered from cryo-archive | $3,088.00 / Non-Profit | Litter | Recovered litter4; additional fees for any special requests. |
| Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information. | ||||
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.