Strain Name:
C57BL/6N-Dpp4em1(DPP4)Mbp/Mmucd
Stock Number:
068166-UCD
Citation ID:
RRID:MMRRC_068166-UCD
Other Names:
DPP4 KI, CR11039

Strain Information

Dpp4em1(DPP4)Mbp
Name: dipeptidylpeptidase 4; endonuclease mediated mutation 1, Mouse Biology Program, University of California, Davis
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
Dpp4
Name: dipeptidylpeptidase 4
Synonyms: THAM, Dpp-4, Cd26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
NCBI: 13482
HGNC: HGNC:3009
Homologene: 3279
Genetic Alterations

In this model, exons 2-26 of human DPP4 have been inserted in-frame into exon 2 of the mouse Dpp4 gene. The insert contains four silent mutations in the coding sequence to prevent CRISPR RNP re-cleavage (see HGVS nomenclature below); it uses the human 3' UTR to terminate transcription and to prevent potential downstream splicing or RNP re-cleavage. Expression is controlled by the mouse regulatory systems and is predicted to terminate with the transgene’s STOP codon without expression of the endogenous sequence. The expression pattern is similar between mice and humans, and it is especially high in the lungs.

Note: Exon 2 is mouse/human chimeric, replacing the murine sequence from the 10th amino acid on (where 1 = Met/ATG). However, the predicted amino acid sequence of the transgene product is 100% identical to the human protein since the first 13 residues match between mice and man. This chimeric approach was chosen to promote intron 1 stability.

HVGS Nomenclature
  • Genbank RefSeq - mRNA: NM_001935.4
  • Genbank RefSeq, protein: NP_001926.2
  • Variant, nucleic acid level: c.30_34delinsCTTAT
  • Variant, amino acid level, predicted: p.=
  • Check this variant: LUMC Mutalyzer
ES Cell Line
Not applicable
Phenotype
Pending analysis
Strain Development
CRISPR/RNP was used to assist with homologous recombination using a dsDNA repair template directly in zygotes. The founder was backcrossed with C57BL/6NCrl to create germline heterozygous animals. Long-range PCR products were sequenced in-full to confirm the absence of potential off-targets. One additional backcross to C57BL/6NCrl.
Suggested Control Mice
C57BL/6NCrl or wild-type siblings
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Kent Lloyd, D.V.M., University of California, Davis.
Primary Reference
Pending publication

Colony and Husbandry Information

Colony's Current Health Status Report

For more information about this colony's health status contact mmrrc@ucdavis.edu
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Generation
N2 (C57BL/6NCrl), F1
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@ucdavis.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A license from third party patent owner(s) may be required for for-profit entities to use mouse models derived from CRISPR-Cas9 technologies and it is the Users sole responsibility to determine whether such a license is necessary.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068166-UCD-HOM-F
068166-UCD-HOM-M
Homozygous Female
Homozygous Male
$272.50 / $674.71
Non-Profit / For-Profit
Per Mouse The mmrrc@ucdavis.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
available service Rederivation to a Barrier Facility $2,800.00 / $6,003.46
Non-Profit / For-Profit
Per Mouse These mice were derived as part of a supplement project for generation of COVID models at the Mouse Biology Program (MBP) at UC Davis. To ensure rapid availability they are being offered as live mice from the MBP production facility, which is not within the M3 Barrier Facility that the MMRRC at UC Davis normally distributes from. Therefore if your institution has approved MMRRC at UC Davis as a vendor, we recommend selecting Rederivation to a Barrier Facility, or discussing this with your animal health coordinator prior to placing the order 1
068166-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.