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Strain Name:
C57BL/6J-MtgxR8558Btlr/Mmmh
Stock Number:
068521-MU
Citation ID:
RRID:MMRRC_068521-MU
Other Names:
R8558 (G1)
Major Collection:

Strain Information

Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Wdr73
Name: WD repeat domain 73
Synonyms: 1200011I23Rik, 2410008B13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71968
Homologene: 41899
Pmpca
Name: peptidase (mitochondrial processing) alpha
Synonyms: INPP5E, Alpha-MPP, 4933435E07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66865
Homologene: 6078
Matk
Name: megakaryocyte-associated tyrosine kinase
Synonyms: Ntk, HYL, CHK, Csk homologous kinase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17179
HGNC: HGNC:6906
Homologene: 48104
Smarcad1
Name: SNF2 related chromatin remodeling ATPase with DExD box 1
Synonyms: D6Pas1, Etl1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13990
Homologene: 5301
Rps6ka2
Name: ribosomal protein S6 kinase, polypeptide 2
Synonyms: Rsk3, Rps6ka-rs1, 90kDa, p90rsk, pp90rsk, D17Wsu134e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20112
VEGA: 17
Homologene: 100680
Wasf1
Name: WASP family, member 1
Synonyms: WAVE-1, Scar, WAVE
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 83767
Homologene: 2920
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 38,044,714 bp
  • T to A, chromosome 1 at 155,221,339 bp
  • T to A, chromosome 1 at 166,487,105 bp
  • C to A, chromosome 2 at 25,680,706 bp
  • G to C, chromosome 2 at 26,395,034 bp
  • A to T, chromosome 2 at 34,704,481 bp
  • A to G, chromosome 2 at 55,446,863 bp
  • A to T, chromosome 2 at 87,309,239 bp
  • A to G, chromosome 2 at 89,574,985 bp
  • C to G, chromosome 2 at 144,586,388 bp
  • T to A, chromosome 2 at 155,024,989 bp
  • T to C, chromosome 2 at 155,242,808 bp
  • T to A, chromosome 3 at 37,048,601 bp
  • T to A, chromosome 3 at 88,984,406 bp
  • C to T, chromosome 3 at 95,354,668 bp
  • A to T, chromosome 3 at 108,217,688 bp
  • T to A, chromosome 3 at 159,614,792 bp
  • A to G, chromosome 4 at 34,657,199 bp
  • C to A, chromosome 4 at 124,894,984 bp
  • C to T, chromosome 4 at 141,470,370 bp
  • T to C, chromosome 4 at 154,998,934 bp
  • A to G, chromosome 4 at 155,585,387 bp
  • C to A, chromosome 5 at 84,059,116 bp
  • G to T, chromosome 5 at 110,208,555 bp
  • A to G, chromosome 6 at 65,083,924 bp
  • A to T, chromosome 6 at 68,704,652 bp
  • T to A, chromosome 6 at 103,708,429 bp
  • G to A, chromosome 6 at 114,480,603 bp
  • A to G, chromosome 7 at 3,249,481 bp
  • A to G, chromosome 7 at 7,312,656 bp
  • G to A, chromosome 7 at 80,898,506 bp
  • A to T, chromosome 7 at 82,428,392 bp
  • GCG to GCGACGGCGCCG, chromosome 7 at 97,579,907 bp
  • A to G, chromosome 7 at 115,541,798 bp
  • A to G, chromosome 8 at 22,168,795 bp
  • T to A, chromosome 8 at 104,953,126 bp
  • A to T, chromosome 8 at 109,635,380 bp
  • C to A, chromosome 9 at 46,155,448 bp
  • T to A, chromosome 9 at 70,034,714 bp
  • T to A, chromosome 9 at 108,515,223 bp
  • T to A, chromosome 9 at 119,958,769 bp
  • G to C, chromosome 10 at 34,298,265 bp
  • T to G, chromosome 10 at 40,930,652 bp
  • A to C, chromosome 10 at 43,127,892 bp
  • T to C, chromosome 10 at 52,353,484 bp
  • C to T, chromosome 10 at 53,615,972 bp
  • T to C, chromosome 10 at 78,747,201 bp
  • T to C, chromosome 10 at 79,270,633 bp
  • C to A, chromosome 10 at 81,260,931 bp
  • A to T, chromosome 10 at 100,594,635 bp
  • T to A, chromosome 10 at 126,993,877 bp
  • T to C, chromosome 11 at 49,180,792 bp
  • G to A, chromosome 11 at 73,453,483 bp
  • A to G, chromosome 11 at 94,351,797 bp
  • T to C, chromosome 11 at 98,211,089 bp
  • A to T, chromosome 11 at 99,021,723 bp
  • A to T, chromosome 11 at 118,448,323 bp
  • T to C, chromosome 12 at 76,612,787 bp
  • T to C, chromosome 12 at 113,597,458 bp
  • T to C, chromosome 12 at 118,877,831 bp
  • G to A, chromosome 13 at 24,025,880 bp
  • C to T, chromosome 14 at 66,916,523 bp
  • G to T, chromosome 15 at 76,519,643 bp
  • A to G, chromosome 16 at 65,827,958 bp
  • G to A, chromosome 17 at 7,255,917 bp
  • T to C, chromosome 17 at 11,237,585 bp
  • T to C, chromosome 17 at 24,208,929 bp
  • A to T, chromosome 17 at 34,736,567 bp
  • G to T, chromosome 17 at 48,090,329 bp
  • A to G, chromosome 17 at 53,483,849 bp
  • A to C, chromosome 17 at 86,809,468 bp
  • T to A, chromosome 18 at 31,829,894 bp
  • T to A, chromosome 18 at 37,744,184 bp
  • T to A, chromosome 18 at 44,191,681 bp
  • G to T, chromosome 18 at 89,473,942 bp
  • A to G, chromosome 19 at 17,122,238 bp
  • T to C, chromosome 19 at 45,758,072 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8558 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068521-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.