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Strain Name:
C57BL/6J-MtgxR8698Btlr/Mmmh
Stock Number:
068552-MU
Citation ID:
RRID:MMRRC_068552-MU
Other Names:
R8698 (G1)
Major Collection:

Strain Information

Pdpk1
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pkb kinase, Pdk1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18607
Homologene: 37643
Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: F4/80, DD7A5-7, TM7LN3, EGF-TM7, Ly71, Emr1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 87,100,486 bp
  • T to C, chromosome 1 at 88,088,230 bp
  • T to C, chromosome 1 at 118,842,157 bp
  • A to G, chromosome 2 at 24,293,185 bp
  • A to G, chromosome 2 at 35,133,962 bp
  • T to C, chromosome 2 at 36,568,903 bp
  • G to T, chromosome 2 at 66,536,284 bp
  • T to A, chromosome 2 at 69,448,239 bp
  • C to T, chromosome 2 at 69,458,423 bp
  • A to G, chromosome 2 at 71,843,274 bp
  • C to T, chromosome 2 at 113,429,807 bp
  • A to G, chromosome 2 at 120,524,119 bp
  • G to A, chromosome 2 at 151,645,453 bp
  • A to T, chromosome 2 at 155,415,121 bp
  • C to T, chromosome 2 at 164,833,134 bp
  • A to G, chromosome 3 at 59,115,175 bp
  • T to A, chromosome 3 at 89,368,737 bp
  • T to C, chromosome 3 at 133,376,601 bp
  • G to A, chromosome 3 at 145,933,267 bp
  • G to T, chromosome 3 at 151,497,875 bp
  • A to G, chromosome 4 at 15,922,483 bp
  • C to T, chromosome 4 at 33,231,246 bp
  • G to A, chromosome 4 at 45,802,942 bp
  • A to G, chromosome 4 at 86,388,477 bp
  • T to C, chromosome 4 at 136,559,207 bp
  • T to A, chromosome 4 at 148,044,490 bp
  • G to T, chromosome 5 at 31,077,475 bp
  • A to G, chromosome 5 at 96,077,290 bp
  • A to T, chromosome 5 at 107,909,910 bp
  • G to T, chromosome 5 at 108,428,239 bp
  • A to G, chromosome 5 at 109,003,524 bp
  • A to T, chromosome 6 at 3,373,843 bp
  • G to A, chromosome 6 at 47,049,222 bp
  • A to G, chromosome 6 at 77,653,117 bp
  • A to T, chromosome 6 at 83,653,285 bp
  • G to T, chromosome 6 at 115,786,891 bp
  • A to G, chromosome 7 at 12,152,612 bp
  • A to G, chromosome 7 at 16,157,207 bp
  • C to T, chromosome 7 at 19,710,965 bp
  • A to T, chromosome 7 at 27,910,936 bp
  • C to A, chromosome 7 at 41,480,510 bp
  • T to C, chromosome 7 at 43,769,689 bp
  • T to C, chromosome 7 at 83,958,582 bp
  • A to T, chromosome 7 at 106,753,364 bp
  • T to C, chromosome 7 at 135,695,208 bp
  • T to A, chromosome 8 at 71,607,515 bp
  • T to G, chromosome 9 at 35,486,973 bp
  • T to C, chromosome 9 at 54,374,669 bp
  • A to T, chromosome 9 at 65,556,272 bp
  • T to C, chromosome 9 at 70,587,070 bp
  • T to G, chromosome 9 at 118,555,961 bp
  • A to G, chromosome 10 at 5,229,229 bp
  • A to G, chromosome 10 at 53,358,105 bp
  • T to C, chromosome 10 at 86,712,977 bp
  • A to G, chromosome 11 at 59,818,060 bp
  • A to T, chromosome 11 at 84,964,183 bp
  • G to A, chromosome 12 at 55,281,714 bp
  • C to T, chromosome 13 at 8,974,213 bp
  • G to A, chromosome 13 at 21,555,720 bp
  • C to T, chromosome 13 at 24,036,246 bp
  • A to T, chromosome 13 at 77,049,165 bp
  • G to T, chromosome 13 at 97,107,070 bp
  • A to G, chromosome 14 at 4,894,467 bp
  • T to A, chromosome 14 at 5,863,554 bp
  • A to T, chromosome 14 at 26,707,263 bp
  • T to C, chromosome 14 at 27,490,690 bp
  • T to A, chromosome 14 at 61,213,353 bp
  • G to T, chromosome 14 at 79,470,133 bp
  • A to T, chromosome 15 at 8,639,152 bp
  • A to C, chromosome 15 at 73,522,305 bp
  • A to T, chromosome 15 at 85,112,846 bp
  • A to G, chromosome 15 at 91,752,197 bp
  • A to T, chromosome 15 at 94,638,109 bp
  • A to G, chromosome 15 at 102,338,815 bp
  • C to A, chromosome 16 at 94,686,555 bp
  • A to T, chromosome 17 at 24,079,568 bp
  • G to A, chromosome 17 at 28,818,462 bp
  • A to C, chromosome 17 at 30,875,035 bp
  • T to C, chromosome 17 at 57,402,003 bp
  • T to C, chromosome 17 at 74,359,346 bp
  • T to C, chromosome 17 at 83,477,916 bp
  • T to C, chromosome 18 at 12,064,231 bp
  • T to A, chromosome 18 at 23,878,033 bp
  • T to C, chromosome 18 at 67,477,052 bp
  • A to G, chromosome 19 at 8,623,525 bp
  • C to T, chromosome 19 at 43,751,509 bp
  • T to C, chromosome 19 at 45,007,860 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8698 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068552-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.