Strain Name:
C57BL/6J-MtgxR8704Btlr/Mmmh
Stock Number:
068558-MU
Citation ID:
RRID:MMRRC_068558-MU
Other Names:
R8704 (G1)
Major Collection:

Strain Information

Wwp2
Name: WW domain containing E3 ubiquitin protein ligase 2
Synonyms: 1300010O06Rik, AIP2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66894
Homologene: 48490
Chtf8
Name: CTF8, chromosome transmission fidelity factor 8
Synonyms: 5830457O10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 214987
Homologene: 84588
Foxp1
Name: forkhead box P1
Synonyms: 3110052D19Rik, 4932443N09Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108655
HGNC: HGNC:3823
Homologene: 13092
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: hyft, CD221, line 186, IGF-1R, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Etf1
Name: eukaryotic translation termination factor 1
Synonyms: ERF1, TB3-1, SUP45L1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225363
HGNC: HGNC:3477
Homologene: 3475
Gars1
Name: glycyl-tRNA synthetase 1
Synonyms: Gena201, Sgrp23, GENA202, Gars
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 353172
HGNC: HGNC:4162
Homologene: 1547
Usp25
Name: ubiquitin specific peptidase 25
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 30940
VEGA: 16
Homologene: 8374
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Mib2
Name: mindbomb E3 ubiquitin protein ligase 2
Synonyms: 2210008I11Rik, Zzank1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76580
Homologene: 16062
Ptprc
Name: protein tyrosine phosphatase receptor type C
Synonyms: T200, Lyt-4, Ly-5, B220, CD45
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Brf2
Name: BRF2, RNA polymerase III transcription initiation factor 50kDa subunit
Synonyms: BRFU, TFIIIB50, 2700059M06Rik, 5730512K07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66653
Homologene: 10127
Nefm
Name: neurofilament, medium polypeptide
Synonyms: neurofilament-M, Nfm, NF165, NF160, NF-M, Nef3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18040
HGNC: HGNC:7734
Homologene: 137211
Lypd5
Name: Ly6/Plaur domain containing 5
Synonyms: 2310011E23Rik, 2210003I03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76942
Homologene: 18797
Rab9
Name: RAB9, member RAS oncogene family
Synonyms: 2410064E05Rik, SID 99
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 56382
HGNC: HGNC:9792
Homologene: 20900
Heatr6
Name: HEAT repeat containing 6
Synonyms: 2700008B19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217026
Homologene: 11116
Hcrt
Name: hypocretin
Synonyms: orexin B, orexin A
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15171
HGNC: HGNC:4847
Homologene: 1166
Nlrp4a
Name: NLR family, pyrin domain containing 4A
Synonyms: Nalp4a, E330028A19Rik, Nalp-eta
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243880
Homologene: 79696
Fat2
Name: FAT atypical cadherin 2
Synonyms: EMI2, Fath2, mKIAA0811, LOC245827
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Agbl1
Name: ATP/GTP binding protein-like 1
Synonyms: Nna1-l1, EG244071, Ccp4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244071
Homologene: 17552
Haspin
Name: histone H3 associated protein kinase
Synonyms: Gsg2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14841
Homologene: 49236
Rhobtb2
Name: Rho-related BTB domain containing 2
Synonyms: Dbc2, E130206H14Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 246710
VEGA: 14
Homologene: 22873
Wdr31
Name: WD repeat domain 31
Synonyms: 5430402I10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 71354
Homologene: 11360
Vmn1r195
Name: vomeronasal 1 receptor 195
Synonyms: V1ri6
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171257
Homologene: 110880
Pfkfb2
Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Synonyms: 4930568D07Rik, PFK-2/FBPase-2 gene B
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18640
HGNC: HGNC:8873
Homologene: 88554
Rhoc
Name: ras homolog family member C
Synonyms: Arh9, Arhc
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11853
HGNC: HGNC:669
Homologene: 90945
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329942
Homologene: 89034
Tsen15
Name: tRNA splicing endonuclease subunit 15
Synonyms: 5730449L18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66637
Homologene: 12002
Acap1
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms: Centb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216859
Homologene: 22835
Mmp19
Name: matrix metallopeptidase 19
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 58223
VEGA: 10
HGNC: HGNC:7165
Homologene: 1820
Pcdhb1
Name: protocadherin beta 1
Synonyms: PcdhbA
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93872
HGNC: HGNC:8680
Homologene: 8348
Tec
Name: tec protein tyrosine kinase
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21682
Homologene: 1302
Sardh
Name: sarcosine dehydrogenase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192166
Homologene: 5149
Or9g4
Name: olfactory receptor family 9 subfamily G member 4
Synonyms: Olfr1006, GA_x6K02T2Q125-47154544-47153606, MOR213-4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258563
Homologene: 17295
Lgr5
Name: leucine rich repeat containing G protein coupled receptor 5
Synonyms: Gpr49
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14160
HGNC: HGNC:4504
Homologene: 20807
Zfp28
Name: zinc finger protein 28
Synonyms: 2810438M17Rik, Zfp-28, mkr-5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22690
Homologene: 69047
Gal3st3
Name: galactose-3-O-sulfotransferase 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 545276
VEGA: 19
Homologene: 57198
Stfa1
Name: stefin A1
Synonyms: Stf1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20861
VEGA: 16
HGNC: HGNC:2481
Homologene: 104045
Gjd3
Name: gap junction protein, delta 3
Synonyms: Gja11, Gjc1, connexin 30.2, connexin-30.2, cx30.2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 353155
Homologene: 17530
Or5p61
Name: olfactory receptor family 5 subfamily P member 61
Synonyms: MOR204-30P, MOR204-40_p, Olfr485, GA_x6K02T2PBJ9-10489044-10488091
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258041
Homologene: 110483
Ugt1a5
Name: UDP glucuronosyltransferase 1 family, polypeptide A5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 394433
Homologene: 88621
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 88,166,365 bp
  • T to C, chromosome 1 at 130,698,043 bp
  • G to A, chromosome 1 at 138,115,624 bp
  • A to T, chromosome 1 at 152,371,790 bp
  • A to G, chromosome 1 at 172,104,126 bp
  • T to C, chromosome 2 at 27,230,465 bp
  • T to A, chromosome 2 at 85,674,218 bp
  • G to C, chromosome 3 at 104,792,026 bp
  • G to A, chromosome 4 at 62,462,205 bp
  • G to A, chromosome 4 at 128,197,354 bp
  • A to G, chromosome 4 at 155,659,163 bp
  • T to C, chromosome 5 at 72,768,762 bp
  • C to T, chromosome 6 at 55,063,230 bp
  • C to G, chromosome 6 at 99,016,546 bp
  • T to C, chromosome 7 at 6,394,638 bp
  • A to G, chromosome 7 at 24,351,557 bp
  • A to T, chromosome 7 at 26,457,138 bp
  • T to C, chromosome 7 at 68,170,054 bp
  • G to T, chromosome 7 at 76,589,554 bp
  • A to T, chromosome 7 at 108,159,504 bp
  • A to G, chromosome 8 at 27,128,471 bp
  • A to C, chromosome 8 at 106,886,040 bp
  • A to T, chromosome 8 at 107,485,596 bp
  • A to G, chromosome 10 at 115,452,705 bp
  • G to A, chromosome 10 at 128,798,834 bp
  • T to A, chromosome 11 at 55,281,311 bp
  • T to C, chromosome 11 at 69,882,663 bp
  • T to C, chromosome 11 at 73,138,002 bp
  • T to A, chromosome 11 at 83,777,278 bp
  • C to T, chromosome 11 at 98,982,619 bp
  • A to T, chromosome 11 at 100,761,926 bp
  • A to G, chromosome 13 at 22,278,888 bp
  • T to C, chromosome 14 at 68,121,061 bp
  • G to A, chromosome 14 at 69,793,924 bp
  • A to T, chromosome 16 at 36,285,174 bp
  • A to G, chromosome 16 at 77,059,290 bp
  • T to C, chromosome 18 at 34,906,086 bp
  • T to A, chromosome 18 at 37,266,349 bp
  • T to C, chromosome 19 at 5,302,635 bp
  • C to T, chromosome X at 166,457,758 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8704 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068558-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.