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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8708Btlr/Mmmh
Stock Number:
068562-MU
Citation ID:
RRID:MMRRC_068562-MU
Other Names:
R8708 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Ing4
Name: inhibitor of growth family, member 4
Synonyms: D6Xrf92, p29ING4, D6Wsu147e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28019
Homologene: 22952

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Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251

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Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659

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Mcoln3
Name: mucolipin 3
Synonyms: varitint-waddler, Va, 6720490O21Rik, TRPML3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 171166
Homologene: 10118

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Lasp1
Name: LIM and SH3 protein 1
Synonyms: SH3P6, Def-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16796
HGNC: HGNC:6513
Homologene: 4480

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
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IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,534,088 bp
  • T to A, chromosome 1 at 87,126,369 bp
  • G to A, chromosome 1 at 131,345,806 bp
  • A to G, chromosome 1 at 167,078,763 bp
  • T to A, chromosome 2 at 22,291,796 bp
  • C to A, chromosome 2 at 25,577,802 bp
  • T to A, chromosome 2 at 37,219,944 bp
  • T to A, chromosome 2 at 69,459,613 bp
  • C to A, chromosome 2 at 89,575,279 bp
  • T to A, chromosome 2 at 91,010,580 bp
  • T to A, chromosome 2 at 91,595,478 bp
  • A to G, chromosome 2 at 120,703,578 bp
  • T to C, chromosome 2 at 120,866,483 bp
  • T to A, chromosome 2 at 127,141,342 bp
  • T to A, chromosome 2 at 130,675,719 bp
  • T to C, chromosome 2 at 131,561,480 bp
  • T to A, chromosome 2 at 151,589,537 bp
  • T to A, chromosome 2 at 160,754,553 bp
  • A to G, chromosome 3 at 59,252,330 bp
  • C to A, chromosome 3 at 83,128,742 bp
  • C to T, chromosome 3 at 108,867,212 bp
  • A to C, chromosome 3 at 123,528,915 bp
  • C to T, chromosome 3 at 145,545,265 bp
  • A to T, chromosome 3 at 146,140,521 bp
  • A to G, chromosome 4 at 11,208,824 bp
  • A to C, chromosome 4 at 32,725,854 bp
  • T to C, chromosome 4 at 43,534,769 bp
  • T to C, chromosome 4 at 129,676,398 bp
  • A to T, chromosome 4 at 133,681,834 bp
  • A to T, chromosome 4 at 155,160,662 bp
  • T to C, chromosome 5 at 73,132,562 bp
  • A to T, chromosome 5 at 105,220,965 bp
  • A to G, chromosome 5 at 108,486,197 bp
  • C to A, chromosome 5 at 110,202,855 bp
  • A to T, chromosome 5 at 113,744,667 bp
  • G to A, chromosome 5 at 114,393,090 bp
  • A to T, chromosome 5 at 127,596,651 bp
  • G to A, chromosome 5 at 129,595,422 bp
  • A to G, chromosome 5 at 137,463,277 bp
  • G to T, chromosome 5 at 139,425,935 bp
  • A to G, chromosome 5 at 142,485,449 bp
  • T to G, chromosome 6 at 5,115,196 bp
  • G to A, chromosome 6 at 8,234,255 bp
  • A to G, chromosome 6 at 56,897,773 bp
  • A to T, chromosome 6 at 118,627,455 bp
  • A to T, chromosome 6 at 125,047,932 bp
  • T to C, chromosome 7 at 6,065,604 bp
  • T to C, chromosome 7 at 19,395,859 bp
  • A to G, chromosome 7 at 45,709,801 bp
  • A to T, chromosome 7 at 86,163,268 bp
  • A to G, chromosome 7 at 101,510,381 bp
  • T to G, chromosome 7 at 105,694,280 bp
  • A to G, chromosome 7 at 129,599,056 bp
  • A to G, chromosome 7 at 141,232,533 bp
  • A to G, chromosome 8 at 13,295,998 bp
  • T to C, chromosome 8 at 22,065,113 bp
  • T to A, chromosome 8 at 33,292,643 bp
  • C to A, chromosome 8 at 54,640,092 bp
  • T to C, chromosome 8 at 70,617,809 bp
  • A to T, chromosome 8 at 89,032,855 bp
  • G to T, chromosome 8 at 129,136,614 bp
  • T to A, chromosome 9 at 53,375,796 bp
  • C to A, chromosome 9 at 81,927,542 bp
  • T to A, chromosome 9 at 102,525,391 bp
  • T to G, chromosome 9 at 108,947,040 bp
  • G to A, chromosome 10 at 80,691,384 bp
  • A to T, chromosome 10 at 128,048,074 bp
  • T to A, chromosome 10 at 129,238,809 bp
  • A to G, chromosome 11 at 3,350,763 bp
  • G to T, chromosome 11 at 16,867,300 bp
  • A to T, chromosome 11 at 85,846,897 bp
  • A to T, chromosome 11 at 89,503,930 bp
  • A to G, chromosome 11 at 97,452,412 bp
  • A to G, chromosome 11 at 97,806,883 bp
  • C to A, chromosome 11 at 107,073,313 bp
  • T to A, chromosome 11 at 107,073,314 bp
  • T to A, chromosome 12 at 4,961,253 bp
  • T to C, chromosome 12 at 80,759,117 bp
  • A to T, chromosome 12 at 104,728,445 bp
  • T to A, chromosome 12 at 105,669,428 bp
  • T to A, chromosome 12 at 115,742,335 bp
  • C to G, chromosome 14 at 32,967,532 bp
  • A to C, chromosome 14 at 55,075,052 bp
  • A to T, chromosome 14 at 59,244,813 bp
  • G to T, chromosome 14 at 64,823,640 bp
  • A to G, chromosome 14 at 67,767,371 bp
  • T to A, chromosome 15 at 27,732,546 bp
  • T to A, chromosome 15 at 68,084,429 bp
  • C to A, chromosome 15 at 101,052,853 bp
  • A to G, chromosome 16 at 18,624,872 bp
  • T to C, chromosome 16 at 97,816,866 bp
  • T to A, chromosome 17 at 14,955,999 bp
  • T to A, chromosome 17 at 20,462,425 bp
  • A to T, chromosome 17 at 24,442,292 bp
  • A to T, chromosome 17 at 24,529,798 bp
  • T to C, chromosome 17 at 34,625,639 bp
  • A to C, chromosome 17 at 65,667,864 bp
  • A to T, chromosome 17 at 84,574,993 bp
  • T to A, chromosome 18 at 36,594,291 bp
  • A to G, chromosome 18 at 63,093,015 bp
  • C to T, chromosome 19 at 5,660,952 bp
  • T to C, chromosome 19 at 6,011,298 bp
  • T to C, chromosome 19 at 13,634,037 bp
  • A to G, chromosome 19 at 18,983,416 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8708 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068562-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.