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Strain Name:
C57BL/6J-MtgxR8714Btlr/Mmmh
Stock Number:
068568-MU
Citation ID:
RRID:MMRRC_068568-MU
Other Names:
R8714 (G1)
Major Collection:

Strain Information

S1pr1
Name: sphingosine-1-phosphate receptor 1
Synonyms: S1P1, S1P, Edg1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13609
HGNC: HGNC:3165
Homologene: 1071
Casp8
Name: caspase 8
Synonyms: Mch5, MACH, FLICE, Caspase-8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12370
HGNC: HGNC:1509
Homologene: 7657
Card11
Name: caspase recruitment domain family, member 11
Synonyms: CARMA1, BIMP3, 2410011D02Rik, 0610008L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024
Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Tyw1
Name: tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
Synonyms: Rsafd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100929
Homologene: 7068
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 12,807,917 bp
  • A to T, chromosome 1 at 58,833,653 bp
  • T to C, chromosome 1 at 71,030,827 bp
  • T to C, chromosome 1 at 155,860,731 bp
  • T to C, chromosome 2 at 60,334,485 bp
  • T to C, chromosome 2 at 118,462,284 bp
  • G to T, chromosome 3 at 41,746,563 bp
  • G to A, chromosome 3 at 89,231,514 bp
  • A to T, chromosome 3 at 115,711,821 bp
  • A to G, chromosome 3 at 122,148,879 bp
  • A to G, chromosome 3 at 153,850,676 bp
  • C to T, chromosome 4 at 46,395,904 bp
  • A to T, chromosome 4 at 89,691,445 bp
  • C to T, chromosome 4 at 103,242,896 bp
  • T to A, chromosome 4 at 130,054,168 bp
  • T to C, chromosome 4 at 141,488,003 bp
  • C to A, chromosome 4 at 148,041,818 bp
  • A to T, chromosome 5 at 6,772,378 bp
  • T to C, chromosome 5 at 90,461,015 bp
  • G to A, chromosome 5 at 130,269,224 bp
  • T to A, chromosome 5 at 140,913,392 bp
  • A to G, chromosome 5 at 144,176,058 bp
  • T to A, chromosome 6 at 32,163,444 bp
  • T to C, chromosome 6 at 88,012,387 bp
  • C to T, chromosome 6 at 121,882,722 bp
  • G to A, chromosome 6 at 132,364,088 bp
  • C to A, chromosome 7 at 5,675,630 bp
  • T to A, chromosome 7 at 30,720,588 bp
  • T to C, chromosome 7 at 83,793,274 bp
  • C to T, chromosome 7 at 96,905,941 bp
  • T to A, chromosome 7 at 104,068,276 bp
  • T to A, chromosome 7 at 141,513,765 bp
  • CAT to C, chromosome 8 at 3,873,772 bp
  • G to T, chromosome 8 at 22,772,232 bp
  • A to G, chromosome 8 at 78,333,702 bp
  • C to T, chromosome 8 at 125,773,807 bp
  • C to T, chromosome 9 at 109,194,764 bp
  • C to T, chromosome 11 at 3,675,877 bp
  • G to T, chromosome 11 at 69,848,012 bp
  • G to C, chromosome 11 at 78,032,666 bp
  • C to A, chromosome 11 at 116,347,065 bp
  • C to T, chromosome 11 at 119,468,894 bp
  • T to A, chromosome 11 at 121,196,782 bp
  • T to C, chromosome 13 at 55,534,437 bp
  • C to T, chromosome 14 at 26,901,075 bp
  • T to A, chromosome 14 at 36,096,499 bp
  • A to G, chromosome 15 at 66,684,042 bp
  • T to C, chromosome 15 at 77,404,742 bp
  • G to A, chromosome 15 at 79,127,310 bp
  • G to A, chromosome 15 at 98,632,381 bp
  • T to A, chromosome 16 at 32,367,411 bp
  • A to T, chromosome 17 at 12,928,629 bp
  • T to C, chromosome 17 at 35,343,362 bp
  • T to C, chromosome 19 at 6,855,845 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8714 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068568-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.