Strain Name:
C57BL/6J-MtgxR8729Btlr/Mmmh
Stock Number:
068577-MU
Citation ID:
RRID:MMRRC_068577-MU
Other Names:
R8729 (G1)
Major Collection:

Strain Information

Pck1
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: PEPCK, Pck-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18534
HGNC: HGNC:8724
Homologene: 1944
Sirt1
Name: sirtuin 1
Synonyms: Sir2, Sir2alpha
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 93759
Homologene: 56556
Ndnf
Name: neuron-derived neurotrophic factor
Synonyms: epidermacan, A930038C07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68169
Homologene: 11593
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Prkd2
Name: protein kinase D2
Synonyms: PKD2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101540
Homologene: 9516
Ccz1
Name: CCZ1 vacuolar protein trafficking and biogenesis associated
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231874
Homologene: 56717
Helz
Name: helicase with zinc finger domain
Synonyms: 3110078M01Rik, 9630002H22Rik, 9430093I07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78455
Homologene: 8918
Hif1a
Name: hypoxia inducible factor 1, alpha subunit
Synonyms: MOP1, bHLHe78, HIF1alpha, HIF-1alpha
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 15251
HGNC: HGNC:4910
Homologene: 1171
Tcof1
Name: treacle ribosome biogenesis factor 1
Synonyms: treacle
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 21453
Homologene: 68049
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, 2900054D09Rik, Ankyrin-3, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Niban2
Name: niban apoptosis regulator 2
Synonyms: Fam129b, 9130404D14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227737
Homologene: 11269
Trp53bp2
Name: transformation related protein 53 binding protein 2
Synonyms: ASPP2, 53BP2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 209456
Homologene: 3959
Nfs1
Name: nitrogen fixation gene 1 (S. cerevisiae)
Synonyms: m-Nfs1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18041
Homologene: 5463
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: enaptin165, nesprin-1, A330049M09Rik, C130039F11Rik, SYNE-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Zkscan5
Name: zinc finger with KRAB and SCAN domains 5
Synonyms: hKraba1, Zfp95
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22757
Homologene: 8734
Polr3c
Name: polymerase (RNA) III (DNA directed) polypeptide C
Synonyms: RPC62, 4933407E01Rik, RPC3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74414
Homologene: 38185
Twf1
Name: twinfilin actin binding protein 1
Synonyms: actin monomer-binding protein, A6, Twinfilin-1, twinfilin, Ptk9
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19230
HGNC: HGNC:9620
Homologene: 48140
Sp2
Name: Sp2 transcription factor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78912
Homologene: 2340
Muc16
Name: mucin 16
Synonyms: 1110008I14Rik, LOC385009, Gm21044
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 73732
Homologene: 141193
Eif2ak3
Name: eukaryotic translation initiation factor 2 alpha kinase 3
Synonyms: PERK
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13666
HGNC: HGNC:3255
Homologene: 3557
Nmd3
Name: NMD3 ribosome export adaptor
Synonyms: C87860
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 97112
Homologene: 6127
Calcb
Name: calcitonin-related polypeptide, beta
Synonyms: CGRP2, Calc2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 116903
HGNC: HGNC:1438
Homologene: 137656
Kat2a
Name: K(lysine) acetyltransferase 2A
Synonyms: 1110051E14Rik, Gcn5l2, Gcn5, PCAF-B/GCN5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14534
HGNC: HGNC:4201
Homologene: 41343
Ccr1
Name: C-C motif chemokine receptor 1
Synonyms: Cmkbr1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12768
VEGA: 9
HGNC: HGNC:1602
Homologene: 20344
Pkd2l2
Name: polycystic kidney disease 2-like 2
Synonyms: TRPP5, Polycystin - L2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 53871
VEGA: 18
HGNC: HGNC:9012
Homologene: 22812
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 667772
VEGA: 16
Homologene: 18929
Csn1s1
Name: casein alpha s1
Synonyms: Csna
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12990
HGNC: HGNC:2445
Srp72
Name: signal recognition particle 72
Synonyms: 72kDa, 5730576P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66661
Homologene: 38254
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329178
Homologene: 122243
Tbx15
Name: T-box 15
Synonyms: Tbx14, de, Tbx8
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21384
Homologene: 7967
Galnt18
Name: polypeptide N-acetylgalactosaminyltransferase 18
Synonyms: Galntl4, 2900011G21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233733
Homologene: 18347
Nlrp9c
Name: NLR family, pyrin domain containing 9C
Synonyms: Nalp-zeta, Nalp9c
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330490
Homologene: 116072
Ankrd61
Name: ankyrin repeat domain 61
Synonyms: 4921520G13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66729
Homologene: 87028
Col14a1
Name: collagen, type XIV, alpha 1
Synonyms: 5730412L22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12818
HGNC: HGNC:2191
Homologene: 18741
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Gpat2
Name: glycerol-3-phosphate acyltransferase 2, mitochondrial
Synonyms: Gpat2, A530057A03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 215456
Homologene: 19037
Krt78
Name: keratin 78
Synonyms: 2310030B04Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 332131
VEGA: 15
Homologene: 65261
Mybpc2
Name: myosin binding protein C, fast-type
Synonyms: Fast-type C-protein
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233199
HGNC: HGNC:7550
Homologene: 3331
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329942
Homologene: 89034
Msrb3
Name: methionine sulfoxide reductase B3
Synonyms: D430026P16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320183
Homologene: 71327
Vmn1r235
Name: vomeronasal 1 receptor 235
Synonyms: V1rf2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171233
Homologene: 74360
Pcdhb9
Name: protocadherin beta 9
Synonyms: Pcdhb4C, PcdhbI
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93880
HGNC: HGNC:8689
Homologene: 87124
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209683
Homologene: 41023
Tmprss9
Name: transmembrane protease, serine 9
Synonyms: Serase-1B
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 432478
VEGA: 10
Homologene: 115302
Gramd1a
Name: GRAM domain containing 1A
Synonyms: D7Bwg0611e, 1300003M23Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 52857
Homologene: 10843
Tbxas1
Name: thromboxane A synthase 1, platelet
Synonyms: TXAS, TXS, CYP5A1, CYP5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21391
Homologene: 130979
Klk1b4
Name: kallikrein 1-related pepidase b4
Synonyms: mGk-4, Ngfa, Ngfa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18048
Homologene: 68141
Luzp2
Name: leucine zipper protein 2
Synonyms: 9330154K17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233271
Homologene: 45618
Or5ae1
Name: olfactory receptor family 5 subfamily AE member 1
Synonyms: Olfr290, MOR254-1, GA_x6K02T2NHDJ-11170115-11169168
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258411
Homologene: 83130
Man2b2
Name: mannosidase 2, alpha B2
Synonyms: 135 kDa alpha-D-mannosidase
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17160
Homologene: 7411
Adam4
Name: a disintegrin and metallopeptidase domain 4
Synonyms: tMDCV
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 11498
Homologene: 86950
Rxfp4
Name: relaxin family peptide receptor 4
Synonyms: Rln3r2, Gpr100
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242093
Homologene: 18775
Trat1
Name: T cell receptor associated transmembrane adaptor 1
Synonyms: Trim, Tcrim, C030046M14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 77647
VEGA: 16
Homologene: 41130
Depp1
Name: DEPP1 autophagy regulator
Synonyms: 8430408G22Rik, Depp
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 213393
Homologene: 48491
Lsm11
Name: U7 snRNP-specific Sm-like protein LSM11
Synonyms: 2210404M20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72290
Homologene: 32693
Rpp21
Name: ribonuclease P 21 subunit
Synonyms: Rpp21, 0610037N01Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67676
Homologene: 41592
Speer4a3
Name: spermatogenesis associated glutamate (E)-rich protein 4A3
Synonyms: Gm21671
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100862359
Homologene: 69402
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 66,608,490 bp
  • A to T, chromosome 1 at 182,449,022 bp
  • T to A, chromosome 2 at 32,909,934 bp
  • AC to A, chromosome 2 at 76,919,184 bp
  • C to A, chromosome 2 at 127,433,819 bp
  • G to A, chromosome 2 at 156,123,807 bp
  • A to G, chromosome 2 at 173,156,073 bp
  • A to T, chromosome 3 at 69,748,349 bp
  • T to A, chromosome 3 at 88,651,998 bp
  • C to T, chromosome 3 at 96,727,480 bp
  • A to T, chromosome 3 at 99,313,060 bp
  • G to A, chromosome 4 at 128,462,845 bp
  • A to G, chromosome 5 at 25,953,180 bp
  • T to C, chromosome 5 at 36,816,118 bp
  • C to T, chromosome 5 at 76,994,158 bp
  • T to C, chromosome 5 at 87,677,139 bp
  • C to T, chromosome 5 at 90,295,593 bp
  • T to A, chromosome 5 at 111,235,643 bp
  • A to G, chromosome 5 at 143,890,985 bp
  • T to C, chromosome 5 at 144,011,492 bp
  • T to A, chromosome 5 at 145,220,261 bp
  • G to T, chromosome 6 at 39,001,338 bp
  • A to T, chromosome 6 at 65,703,774 bp
  • C to A, chromosome 6 at 70,844,880 bp
  • C to A, chromosome 6 at 116,651,801 bp
  • T to A, chromosome 7 at 16,849,127 bp
  • T to G, chromosome 7 at 26,372,003 bp
  • C to A, chromosome 7 at 31,143,823 bp
  • T to A, chromosome 7 at 44,207,460 bp
  • C to T, chromosome 7 at 44,506,187 bp
  • A to G, chromosome 7 at 55,167,237 bp
  • T to C, chromosome 7 at 84,916,315 bp
  • T to C, chromosome 7 at 111,519,991 bp
  • A to C, chromosome 7 at 114,720,193 bp
  • T to C, chromosome 9 at 18,660,050 bp
  • T to C, chromosome 9 at 123,963,794 bp
  • T to A, chromosome 10 at 5,229,275 bp
  • A to G, chromosome 10 at 63,320,926 bp
  • A to G, chromosome 10 at 70,002,598 bp
  • A to T, chromosome 10 at 80,890,343 bp
  • A to C, chromosome 10 at 120,852,069 bp
  • C to T, chromosome 11 at 45,944,900 bp
  • T to C, chromosome 11 at 96,961,273 bp
  • T to C, chromosome 11 at 100,710,511 bp
  • G to T, chromosome 11 at 107,637,928 bp
  • A to G, chromosome 12 at 73,944,128 bp
  • A to T, chromosome 12 at 81,421,402 bp
  • A to T, chromosome 12 at 114,196,632 bp
  • T to A, chromosome 15 at 55,447,497 bp
  • T to C, chromosome 15 at 94,581,331 bp
  • T to G, chromosome 15 at 101,947,020 bp
  • A to T, chromosome 16 at 48,742,228 bp
  • A to G, chromosome 16 at 49,061,488 bp
  • A to T, chromosome 17 at 21,261,613 bp
  • A to T, chromosome 17 at 22,548,258 bp
  • A to G, chromosome 17 at 36,256,035 bp
  • G to A, chromosome 18 at 34,433,301 bp
  • C to A, chromosome 18 at 37,402,586 bp
  • G to T, chromosome 18 at 60,829,073 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8729 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068577-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.