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Strain Name:
C57BL/6J-MtgxR8733Btlr/Mmmh
Stock Number:
068581-MU
Citation ID:
RRID:MMRRC_068581-MU
Other Names:
R8733 (G1)
Major Collection:

Strain Information

Hgs
Name: HGF-regulated tyrosine kinase substrate
Synonyms: Hrs, tn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15239
HGNC: HGNC:4897
Homologene: 37954
Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Lima1
Name: LIM domain and actin binding 1
Synonyms: 1110021C24Rik, 3526402A12Rik, epithelial protein lost in neoplasm, EPLIN
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 65970
Homologene: 9484
Cep85
Name: centrosomal protein 85
Synonyms: 2410030J07Rik, Ccdc21
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70012
Homologene: 11254
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,552,873 bp
  • A to G, chromosome 1 at 45,340,312 bp
  • G to T, chromosome 2 at 32,316,975 bp
  • C to A, chromosome 2 at 33,284,824 bp
  • A to G, chromosome 2 at 36,134,199 bp
  • T to A, chromosome 2 at 66,324,600 bp
  • A to T, chromosome 2 at 87,284,772 bp
  • G to A, chromosome 2 at 146,424,763 bp
  • A to G, chromosome 3 at 7,528,108 bp
  • C to G, chromosome 3 at 107,604,819 bp
  • A to T, chromosome 4 at 59,958,587 bp
  • C to A, chromosome 4 at 132,338,204 bp
  • T to C, chromosome 4 at 134,148,161 bp
  • A to T, chromosome 4 at 152,379,466 bp
  • G to A, chromosome 5 at 38,299,773 bp
  • T to A, chromosome 5 at 38,300,453 bp
  • A to T, chromosome 6 at 90,633,728 bp
  • C to T, chromosome 6 at 139,768,700 bp
  • A to T, chromosome 7 at 29,191,686 bp
  • T to C, chromosome 7 at 73,409,288 bp
  • C to T, chromosome 7 at 110,878,218 bp
  • A to G, chromosome 7 at 141,863,795 bp
  • A to T, chromosome 8 at 47,501,848 bp
  • A to G, chromosome 9 at 22,670,832 bp
  • A to G, chromosome 9 at 38,930,689 bp
  • T to C, chromosome 9 at 72,726,484 bp
  • C to T, chromosome 9 at 106,475,951 bp
  • T to A, chromosome 9 at 114,705,110 bp
  • A to G, chromosome 10 at 40,574,065 bp
  • A to T, chromosome 10 at 78,073,863 bp
  • T to A, chromosome 10 at 129,570,710 bp
  • A to T, chromosome 11 at 8,933,657 bp
  • G to A, chromosome 11 at 43,443,340 bp
  • G to T, chromosome 11 at 50,210,666 bp
  • G to T, chromosome 11 at 61,671,691 bp
  • A to G, chromosome 11 at 70,617,030 bp
  • A to G, chromosome 11 at 80,434,516 bp
  • A to G, chromosome 11 at 94,358,801 bp
  • A to G, chromosome 11 at 113,709,453 bp
  • A to G, chromosome 11 at 116,535,625 bp
  • G to A, chromosome 11 at 120,470,128 bp
  • A to G, chromosome 13 at 67,620,985 bp
  • T to A, chromosome 14 at 52,638,756 bp
  • T to A, chromosome 14 at 53,591,298 bp
  • G to A, chromosome 14 at 62,696,848 bp
  • T to A, chromosome 15 at 36,176,767 bp
  • A to G, chromosome 15 at 99,780,818 bp
  • T to C, chromosome 16 at 18,259,961 bp
  • A to G, chromosome 17 at 6,050,028 bp
  • A to G, chromosome 17 at 35,881,375 bp
  • A to G, chromosome 17 at 38,644,618 bp
  • G to A, chromosome 17 at 49,096,796 bp
  • C to T, chromosome 18 at 37,505,384 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8733 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068581-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.