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Strain Name:
C57BL/6J-MtgxR8750Btlr/Mmmh
Stock Number:
068593-MU
Citation ID:
RRID:MMRRC_068593-MU
Other Names:
R8750 (G1)
Major Collection:

Strain Information

Kif5a
Name: kinesin family member 5A
Synonyms: Kns, Kif5, D10Bwg0738e, Khc
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16572
VEGA: 10
HGNC: HGNC:6323
Homologene: 55861
Foxm1
Name: forkhead box M1
Synonyms: Mpm2, WIN, HFH-11B, Trident, Fkh16, Foxm1b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14235
HGNC: HGNC:3818
Homologene: 7318
Avpr1b
Name: arginine vasopressin receptor 1B
Synonyms: V3/V1b pituitary vasopressin receptor, V3/V1b, V1bR, AVPR3, V1BR, VPR3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26361
HGNC: HGNC:896
Homologene: 22678
Mad1l1
Name: MAD1 mitotic arrest deficient 1-like 1
Synonyms: Mad1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17120
HGNC: HGNC:6762
Homologene: 74500
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Tiparp
Name: TCDD-inducible poly(ADP-ribose) polymerase
Synonyms: DDF1, PARP-7, PARP7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99929
Homologene: 9167
Tac2
Name: tachykinin 2
Synonyms: neuropeptide K, neuromedin L, neurokinin alpha, neurokinin 2, neurokinin A, substance K
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21334
VEGA: 10
Homologene: 7560
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 86,088,863 bp
  • C to T, chromosome 1 at 131,599,936 bp
  • T to C, chromosome 2 at 18,308,834 bp
  • T to A, chromosome 2 at 44,997,927 bp
  • T to A, chromosome 2 at 85,769,963 bp
  • T to G, chromosome 2 at 135,335,449 bp
  • T to C, chromosome 2 at 148,443,160 bp
  • T to A, chromosome 2 at 164,565,898 bp
  • A to T, chromosome 3 at 36,607,595 bp
  • T to G, chromosome 3 at 65,552,704 bp
  • C to T, chromosome 3 at 119,141,936 bp
  • A to T, chromosome 4 at 56,886,141 bp
  • C to G, chromosome 4 at 108,550,743 bp
  • G to A, chromosome 4 at 116,599,932 bp
  • T to C, chromosome 4 at 126,238,522 bp
  • T to A, chromosome 4 at 131,922,095 bp
  • A to G, chromosome 5 at 3,702,890 bp
  • A to G, chromosome 5 at 23,493,217 bp
  • A to G, chromosome 5 at 116,467,508 bp
  • A to T, chromosome 5 at 139,050,696 bp
  • T to A, chromosome 5 at 140,315,067 bp
  • T to A, chromosome 6 at 29,931,816 bp
  • T to C, chromosome 6 at 48,405,278 bp
  • T to A, chromosome 6 at 71,509,618 bp
  • T to G, chromosome 6 at 83,183,126 bp
  • T to A, chromosome 6 at 112,382,351 bp
  • T to C, chromosome 6 at 113,686,822 bp
  • T to A, chromosome 6 at 128,373,243 bp
  • T to C, chromosome 6 at 128,412,792 bp
  • A to G, chromosome 7 at 26,312,784 bp
  • T to C, chromosome 7 at 29,895,379 bp
  • C to A, chromosome 7 at 35,376,255 bp
  • G to T, chromosome 7 at 44,305,899 bp
  • T to C, chromosome 7 at 48,482,030 bp
  • C to T, chromosome 7 at 89,307,248 bp
  • T to A, chromosome 7 at 103,639,852 bp
  • A to G, chromosome 7 at 127,050,346 bp
  • A to T, chromosome 8 at 69,820,484 bp
  • A to T, chromosome 8 at 84,212,684 bp
  • C to A, chromosome 8 at 84,559,155 bp
  • A to T, chromosome 9 at 15,874,428 bp
  • A to C, chromosome 9 at 40,984,345 bp
  • A to G, chromosome 9 at 44,103,505 bp
  • T to C, chromosome 10 at 63,167,257 bp
  • A to G, chromosome 10 at 78,177,825 bp
  • A to G, chromosome 10 at 80,039,590 bp
  • T to A, chromosome 10 at 127,248,040 bp
  • C to T, chromosome 10 at 127,728,450 bp
  • T to C, chromosome 10 at 129,409,780 bp
  • A to T, chromosome 11 at 80,867,688 bp
  • A to C, chromosome 11 at 88,944,472 bp
  • A to T, chromosome 11 at 106,421,950 bp
  • G to A, chromosome 12 at 80,598,105 bp
  • A to T, chromosome 12 at 80,706,087 bp
  • G to A, chromosome 12 at 81,560,473 bp
  • G to T, chromosome 12 at 85,261,714 bp
  • G to T, chromosome 12 at 87,056,532 bp
  • C to T, chromosome 13 at 33,151,810 bp
  • A to T, chromosome 14 at 31,304,967 bp
  • T to C, chromosome 14 at 73,029,638 bp
  • A to G, chromosome 14 at 122,476,717 bp
  • T to C, chromosome 15 at 39,119,441 bp
  • T to G, chromosome 15 at 98,457,048 bp
  • A to C, chromosome 16 at 48,980,975 bp
  • T to C, chromosome 16 at 97,451,717 bp
  • A to T, chromosome 17 at 21,722,375 bp
  • T to C, chromosome 17 at 24,642,259 bp
  • T to C, chromosome 17 at 55,666,537 bp
  • A to T, chromosome 18 at 20,575,012 bp
  • T to C, chromosome 18 at 84,015,037 bp
  • T to A, chromosome 19 at 6,120,563 bp
  • A to C, chromosome 19 at 16,608,094 bp
  • G to A, chromosome 19 at 25,611,186 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8750 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068593-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.