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Strain Name:
C57BL/6J-MtgxR8784Btlr/Mmmh
Stock Number:
068606-MU
Citation ID:
RRID:MMRRC_068606-MU
Other Names:
R8784 (G1)
Major Collection:

Strain Information

Pank3
Name: pantothenate kinase 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211347
Homologene: 56999
Chrm4
Name: cholinergic receptor, muscarinic 4
Synonyms: Chrm-4, muscarinic acetylcholine receptor 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12672
HGNC: HGNC:1953
Homologene: 20192
Dtnbp1
Name: dystrobrevin binding protein 1
Synonyms: dysbindin, 5430437B18Rik, sdy, Bloc1s8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94245
VEGA: 13
Homologene: 12037
Tti1
Name: TELO2 interacting protein 1
Synonyms: 2610036D13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75425
Homologene: 40969
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: FBP2, 9930124L22Rik, Fnbp2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14270
Homologene: 52683
Katna1
Name: katanin p60 (ATPase-containing) subunit A1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 23924
HGNC: HGNC:6216
Homologene: 56014
Gdf3
Name: growth differentiation factor 3
Synonyms: Gdf-3, Vgr-2, Vgr2, ecat9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14562
HGNC: HGNC:4218
Homologene: 7336
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 75,200,331 bp
  • T to C, chromosome 1 at 75,405,149 bp
  • T to C, chromosome 1 at 82,234,281 bp
  • T to C, chromosome 1 at 84,913,063 bp
  • T to A, chromosome 1 at 87,477,571 bp
  • T to A, chromosome 1 at 131,295,474 bp
  • G to A, chromosome 2 at 14,996,495 bp
  • C to G, chromosome 2 at 27,071,935 bp
  • T to A, chromosome 2 at 32,370,250 bp
  • A to T, chromosome 2 at 88,411,747 bp
  • T to A, chromosome 2 at 90,460,512 bp
  • T to A, chromosome 2 at 91,927,688 bp
  • A to G, chromosome 2 at 118,113,132 bp
  • G to A, chromosome 2 at 125,760,343 bp
  • T to C, chromosome 2 at 158,008,594 bp
  • A to T, chromosome 3 at 86,375,928 bp
  • A to T, chromosome 3 at 88,072,726 bp
  • G to A, chromosome 3 at 117,322,541 bp
  • C to T, chromosome 4 at 35,152,383 bp
  • A to T, chromosome 4 at 155,772,888 bp
  • T to C, chromosome 5 at 43,703,303 bp
  • G to T, chromosome 5 at 114,388,739 bp
  • A to T, chromosome 5 at 115,846,383 bp
  • A to G, chromosome 5 at 121,795,028 bp
  • C to T, chromosome 5 at 134,559,869 bp
  • A to T, chromosome 5 at 150,548,661 bp
  • T to A, chromosome 6 at 30,958,114 bp
  • T to G, chromosome 6 at 42,679,287 bp
  • T to A, chromosome 6 at 55,481,115 bp
  • T to A, chromosome 6 at 58,008,455 bp
  • A to G, chromosome 6 at 67,466,417 bp
  • A to T, chromosome 6 at 69,399,962 bp
  • T to G, chromosome 6 at 71,509,998 bp
  • G to T, chromosome 6 at 71,950,628 bp
  • A to T, chromosome 6 at 122,606,320 bp
  • G to A, chromosome 6 at 136,401,731 bp
  • A to G, chromosome 6 at 136,599,650 bp
  • C to A, chromosome 7 at 7,132,571 bp
  • A to T, chromosome 7 at 15,971,950 bp
  • A to G, chromosome 7 at 30,043,536 bp
  • A to T, chromosome 7 at 46,002,601 bp
  • T to C, chromosome 7 at 102,559,468 bp
  • A to C, chromosome 7 at 141,431,107 bp
  • A to G, chromosome 8 at 70,906,265 bp
  • C to A, chromosome 8 at 83,437,616 bp
  • T to A, chromosome 8 at 119,626,231 bp
  • T to A, chromosome 8 at 122,001,113 bp
  • A to T, chromosome 8 at 122,496,589 bp
  • T to A, chromosome 9 at 39,318,693 bp
  • T to G, chromosome 9 at 40,960,886 bp
  • A to T, chromosome 9 at 51,059,481 bp
  • G to T, chromosome 9 at 90,193,865 bp
  • A to G, chromosome 9 at 107,557,842 bp
  • A to G, chromosome 9 at 107,788,138 bp
  • A to T, chromosome 9 at 118,055,892 bp
  • T to A, chromosome 10 at 7,738,815 bp
  • A to G, chromosome 10 at 23,904,628 bp
  • T to G, chromosome 10 at 127,457,652 bp
  • A to T, chromosome 11 at 7,201,952 bp
  • T to G, chromosome 11 at 35,781,585 bp
  • T to C, chromosome 11 at 76,208,115 bp
  • T to C, chromosome 11 at 78,172,549 bp
  • T to A, chromosome 11 at 86,222,137 bp
  • G to A, chromosome 11 at 86,225,115 bp
  • A to G, chromosome 11 at 96,351,910 bp
  • C to T, chromosome 11 at 96,912,386 bp
  • G to T, chromosome 12 at 16,656,851 bp
  • C to T, chromosome 12 at 76,439,193 bp
  • A to C, chromosome 12 at 105,639,241 bp
  • T to C, chromosome 12 at 115,802,860 bp
  • T to A, chromosome 13 at 21,363,821 bp
  • T to C, chromosome 13 at 44,922,226 bp
  • T to A, chromosome 13 at 100,539,542 bp
  • T to C, chromosome 15 at 4,793,140 bp
  • A to G, chromosome 15 at 28,387,951 bp
  • C to A, chromosome 15 at 76,077,676 bp
  • T to C, chromosome 15 at 76,343,663 bp
  • T to A, chromosome 15 at 102,505,362 bp
  • A to T, chromosome 16 at 48,931,265 bp
  • A to G, chromosome 17 at 26,742,546 bp
  • T to C, chromosome 17 at 29,599,944 bp
  • A to G, chromosome 17 at 37,299,810 bp
  • A to G, chromosome 18 at 12,421,155 bp
  • C to A, chromosome 19 at 16,664,789 bp
  • A to G, chromosome 19 at 22,918,676 bp
  • C to T, chromosome 19 at 26,776,158 bp
  • T to C, chromosome 19 at 32,513,996 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8784 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068606-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.