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Strain Name:
C57BL/6J-MtgxR8824Btlr/Mmmh
Stock Number:
068657-MU
Citation ID:
RRID:MMRRC_068657-MU
Other Names:
R8824 (G1)
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Adora2a
Name: adenosine A2a receptor
Synonyms: A2aR, A2AAR, AA2AR, A2a, Rs, ARA2A
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11540
VEGA: 10
HGNC: HGNC:263
Homologene: 20166
Dnmbp
Name: dynamin binding protein
Synonyms: 2410003M15Rik, Tuba, 2410003L07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71972
Homologene: 9061
Cyp11b2
Name: cytochrome P450, family 11, subfamily b, polypeptide 2
Synonyms: aldosterone synthase, Cyp11b-2, Cyp11b, steroid-11-beta-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13072
Homologene: 128035
Gart
Name: phosphoribosylglycinamide formyltransferase
Synonyms: Gaps, Prgs
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14450
HGNC: HGNC:4163
Homologene: 637
Dusp16
Name: dual specificity phosphatase 16
Synonyms: MKP-7, MKP7, 3830417M17Rik, D6Ertd213e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70686
Homologene: 15604
Zeb1
Name: zinc finger E-box binding homeobox 1
Synonyms: Nil2, [delta]EF1, MEB1, Tcf8, Zfx1a, Tcf18, AREB6, ZEB, Zfhep, 3110032K11Rik, Zfhx1a, Tw
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 21417
Homologene: 31779
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 13,177,185 bp
  • T to A, chromosome 1 at 97,128,159 bp
  • T to G, chromosome 1 at 117,947,895 bp
  • T to A, chromosome 1 at 138,113,708 bp
  • A to T, chromosome 2 at 36,735,191 bp
  • C to G, chromosome 2 at 52,216,911 bp
  • T to C, chromosome 2 at 87,671,960 bp
  • A to G, chromosome 2 at 87,974,003 bp
  • A to G, chromosome 2 at 90,296,012 bp
  • A to T, chromosome 2 at 93,862,850 bp
  • A to G, chromosome 2 at 104,991,877 bp
  • A to G, chromosome 2 at 155,630,381 bp
  • T to C, chromosome 3 at 19,672,962 bp
  • A to T, chromosome 3 at 58,884,893 bp
  • C to T, chromosome 4 at 88,557,761 bp
  • C to A, chromosome 4 at 126,507,184 bp
  • A to G, chromosome 4 at 138,735,867 bp
  • A to G, chromosome 4 at 139,653,763 bp
  • G to A, chromosome 4 at 155,344,828 bp
  • G to A, chromosome 5 at 98,942,208 bp
  • A to G, chromosome 5 at 125,118,757 bp
  • T to C, chromosome 5 at 147,334,863 bp
  • T to TCCC, chromosome 6 at 4,756,451 bp
  • C to A, chromosome 6 at 24,479,308 bp
  • A to G, chromosome 6 at 27,761,352 bp
  • A to T, chromosome 6 at 124,517,695 bp
  • A to T, chromosome 6 at 134,739,769 bp
  • T to C, chromosome 7 at 27,963,468 bp
  • A to T, chromosome 7 at 47,235,293 bp
  • C to T, chromosome 7 at 98,443,469 bp
  • T to A, chromosome 7 at 104,357,906 bp
  • T to G, chromosome 7 at 108,523,143 bp
  • A to C, chromosome 8 at 15,114,169 bp
  • A to G, chromosome 8 at 41,199,711 bp
  • A to G, chromosome 8 at 80,705,332 bp
  • C to A, chromosome 9 at 14,727,475 bp
  • A to G, chromosome 9 at 75,167,046 bp
  • A to G, chromosome 9 at 108,967,025 bp
  • A to C, chromosome 9 at 123,810,941 bp
  • G to A, chromosome 10 at 42,438,387 bp
  • G to A, chromosome 10 at 75,326,179 bp
  • A to G, chromosome 10 at 76,278,486 bp
  • A to G, chromosome 10 at 79,637,702 bp
  • C to T, chromosome 10 at 129,965,792 bp
  • A to T, chromosome 11 at 22,232,053 bp
  • T to A, chromosome 11 at 117,739,854 bp
  • C to T, chromosome 13 at 37,930,516 bp
  • A to G, chromosome 13 at 74,363,949 bp
  • G to A, chromosome 13 at 112,701,487 bp
  • A to T, chromosome 14 at 78,516,347 bp
  • T to A, chromosome 15 at 35,533,299 bp
  • C to T, chromosome 15 at 57,821,280 bp
  • T to A, chromosome 15 at 74,856,065 bp
  • C to T, chromosome 15 at 97,766,908 bp
  • T to A, chromosome 15 at 101,920,642 bp
  • A to G, chromosome 16 at 36,915,689 bp
  • A to G, chromosome 16 at 44,215,613 bp
  • A to T, chromosome 16 at 88,647,415 bp
  • T to C, chromosome 16 at 88,647,436 bp
  • T to C, chromosome 16 at 91,630,703 bp
  • A to C, chromosome 17 at 31,273,376 bp
  • T to A, chromosome 18 at 5,748,680 bp
  • T to A, chromosome 18 at 12,686,946 bp
  • A to G, chromosome 19 at 5,069,617 bp
  • T to C, chromosome 19 at 34,084,716 bp
  • A to G, chromosome 19 at 43,849,837 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8824 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068657-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.